Thermosensitization, heat shock protein synthesis and development of thermotolerance in M-14 human tumor cells subjected to step-down heating

Andrea Delpino, Francesco Paolo Gentile, Francesca Di Modugno, Marcello Benassi, Anna Maria Mileo, Elisabetta Mattei

Research output: Contribution to journalArticlepeer-review

Abstract

M-14 human tumor cells have been subjected to two regimens of step-down heating (SDH) consisting of a conditioning treatment at 42 °C for 1 h or at 44.5 °C for 20 min, immediately followed by heating at 40 °C. Both conditioning treatments thermosensitize the cells towards the subsequent heating at 40 °C; the thermosensitization ratio is 6.4 for cells conditioned at 42 °C for 1 h and 32.3 for cells conditioned at 44.5 °C for 20 min. The overall protein synthetic activity is reduced to 32.7% or 18.4% of control values following 1 h at 42 °C and 20 min at 44.5 °C, respectively; this inhibition is followed by a full recovery of the synthetic activity during the subsequent exposure at 40 °C. SDH-treated cells synthetize four heat shock proteins, with approximate molecular weights of 28, 64, 70 and 90 kDa. The pattern of HSPs induction observed in SDH-treted cells is similar to that found in cells subjected to single hyperthermic exposures. Cells subjected to the SDH sequence 42 °C/1 h→40 °C/4 h develop thermotolerance, as indicated by a reduced sensitivity to further hyperthermic challenges.

Original languageEnglish
Pages (from-to)323-332
Number of pages10
JournalRadiation and Environmental Biophysics
Volume31
Issue number4
DOIs
Publication statusPublished - Dec 1992

ASJC Scopus subject areas

  • Radiation
  • Physics and Astronomy (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Environmental Chemistry
  • Environmental Science (miscellaneous)
  • Environmental Science(all)
  • Biophysics
  • Agricultural and Biological Sciences (miscellaneous)

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