Thiazolidinediones and antiblastics in primary human anaplastic thyroid cancer cells

Alessandro Antonelli, Silvia Martina Ferrari, Poupak Fallahi, Piero Berti, Gabriele Materazzi, Michele Minuto, Riccardo Giannini, Ivo Marchetti, Lucio Barani, Fulvio Basolo, Ele Ferrannini, Paolo Miccoli

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Abstract

Objective No study has evaluated the antiproliferative effects of thiazolidinediones and antiblastics in 'primary cultured human anaplastic thyroid cancer cells'. Design Primary anaplastic cells proliferation was evaluated after incubation with increasing concentrations of rosiglitazone or pioglitazone or antiblastics (bleomycin, cisplatin, gemcitabine) by a proliferation assay (WST-1-tetrazolium reaction) and cell counting. Measurements and results A reduction of proliferation by thiazolidinediones at 1 h (from the start of tetrazolium reaction) [of 11% and 25%, with rosiglitazone, 10 or 20 (P = 0·0001) μM, respectively; of 7% and 17%, with pioglitazone, 10 or 20 (P = 0·0125) μM, respectively], and at 2 h [of 14% and 24%, with rosiglitazone, 10 (P = 0·0043) or 20 (P <0·0001) μM, respectively; of 9% and 21%, with pioglitazone, 10 (P = 0·0397) or 20 (P = 0·0001) μM, respectively] was shown. No significant thiazolidinediones effect was observed in normal thyroid follicular cells. Bleomycin, cisplatin and gemcitabine significantly (P <0·0001) inhibited (> 50%) anaplastic cells proliferation. Cell counting confirmed the above mentioned results. Inhibition of proliferation was similar in tumours with or without V600EBRAF mutation, both for thiazolidinediones and antiblastics. Conclusions Thiazolidinediones exert an antiproliferative effect in primary cultured human anaplastic carcinoma cells in vitro, such as antiblastics.

Original languageEnglish
Pages (from-to)946-953
Number of pages8
JournalClinical Endocrinology
Volume70
Issue number6
DOIs
Publication statusPublished - Jun 2009

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

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    Antonelli, A., Ferrari, S. M., Fallahi, P., Berti, P., Materazzi, G., Minuto, M., Giannini, R., Marchetti, I., Barani, L., Basolo, F., Ferrannini, E., & Miccoli, P. (2009). Thiazolidinediones and antiblastics in primary human anaplastic thyroid cancer cells. Clinical Endocrinology, 70(6), 946-953. https://doi.org/10.1111/j.1365-2265.2008.03415.x