Abstract
In vitro assays with endothelial cells (EC) cultured on three-dimensional gel recapitulate several aspects of vascular morphogenesis and pathological angiogenesis. The two most used in vitro assays of vascular mor- phogenesis are the tube formation on extracellular matrix gel and the sprouting from EC spheroids. Tube formation assay measures the ability of EC, plated on gel derived from reconstituted basement membrane, to form capillary-like structures. Sprouting assay is based on spheroids of EC, embedded in collagen gel and stimulated with angiogenic factors, which originate a complex network of capillary-like structures invading the gel. Both these assays can be exploited for antiangiogenic drug screening and gene function analysis during vascular morphogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 41-47 |
| Number of pages | 7 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 1214 |
| DOIs | |
| Publication status | Published - 2015 |
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Keywords
- Basement membrane extract (Matrigel)
- Capillarylike structures
- Collagen type-I
- HUVEC
- Spheroids
- VEGF-A
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Three-dimensional in vitro assay of endothelial cell invasion and capillary tube morphogenesis. / Di Blasio, Laura; Bussolino, Federico; Primo, Luca.
In: Methods in molecular biology (Clifton, N.J.), Vol. 1214, 2015, p. 41-47.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Three-dimensional in vitro assay of endothelial cell invasion and capillary tube morphogenesis
AU - Di Blasio, Laura
AU - Bussolino, Federico
AU - Primo, Luca
PY - 2015
Y1 - 2015
N2 - In vitro assays with endothelial cells (EC) cultured on three-dimensional gel recapitulate several aspects of vascular morphogenesis and pathological angiogenesis. The two most used in vitro assays of vascular mor- phogenesis are the tube formation on extracellular matrix gel and the sprouting from EC spheroids. Tube formation assay measures the ability of EC, plated on gel derived from reconstituted basement membrane, to form capillary-like structures. Sprouting assay is based on spheroids of EC, embedded in collagen gel and stimulated with angiogenic factors, which originate a complex network of capillary-like structures invading the gel. Both these assays can be exploited for antiangiogenic drug screening and gene function analysis during vascular morphogenesis.
AB - In vitro assays with endothelial cells (EC) cultured on three-dimensional gel recapitulate several aspects of vascular morphogenesis and pathological angiogenesis. The two most used in vitro assays of vascular mor- phogenesis are the tube formation on extracellular matrix gel and the sprouting from EC spheroids. Tube formation assay measures the ability of EC, plated on gel derived from reconstituted basement membrane, to form capillary-like structures. Sprouting assay is based on spheroids of EC, embedded in collagen gel and stimulated with angiogenic factors, which originate a complex network of capillary-like structures invading the gel. Both these assays can be exploited for antiangiogenic drug screening and gene function analysis during vascular morphogenesis.
KW - Basement membrane extract (Matrigel)
KW - Capillarylike structures
KW - Collagen type-I
KW - HUVEC
KW - Spheroids
KW - VEGF-A
UR - http://www.scopus.com/inward/record.url?scp=84916210373&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84916210373&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-1462-3_4
DO - 10.1007/978-1-4939-1462-3_4
M3 - Article
C2 - 25468598
VL - 1214
SP - 41
EP - 47
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -