Three-dimensional localization and dynamics of centromeres in mouse oocytes during folliculogenesis

Silvia Garagna, Valeria Merico, Vittorio Sebastiano, Manuela Monti, Guido Orlandini, Rita Gatti, Renato Scandroglio, Carlo Alberto Redi, Maurizio Zuccotti

Research output: Contribution to journalArticle

Abstract

Very little is known about oocyte nuclear architecture during folliculogenesis. Using antibodies to reveal centromeres, Hoechst-staining to detect the AT-rich pericentromeric heterochromatin (chromocenters), combined with confocal microscopy for the three-dimensional analysis of the nucleus, we demonstrate that during mouse folliculogenesis the oocyte nuclear architecture undergoes dynamic changes. In oocytes isolated from primordial and primary follicles, centromeres and chromocenters were preferentially located at the periphery of the nucleus. During oocyte growth, centromeres and chromocenters were initially found spread within the nucleus and then progressively clustered around the periphery of the nucleolus. Our results indicate that the oocyte nuclear achitecture is developmentally regulated and they contribute to a further understanding of the role of nuclear organization in the regulation of genome functioning during differentiation and development.

Original languageEnglish
Pages (from-to)631-638
Number of pages8
JournalJournal of Molecular Histology
Volume35
Issue number6
DOIs
Publication statusPublished - Aug 2004

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Centromere
Oocytes
Heterochromatin
Confocal Microscopy
Genome
Staining and Labeling
Antibodies
Growth

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Three-dimensional localization and dynamics of centromeres in mouse oocytes during folliculogenesis. / Garagna, Silvia; Merico, Valeria; Sebastiano, Vittorio; Monti, Manuela; Orlandini, Guido; Gatti, Rita; Scandroglio, Renato; Redi, Carlo Alberto; Zuccotti, Maurizio.

In: Journal of Molecular Histology, Vol. 35, No. 6, 08.2004, p. 631-638.

Research output: Contribution to journalArticle

Garagna, S, Merico, V, Sebastiano, V, Monti, M, Orlandini, G, Gatti, R, Scandroglio, R, Redi, CA & Zuccotti, M 2004, 'Three-dimensional localization and dynamics of centromeres in mouse oocytes during folliculogenesis', Journal of Molecular Histology, vol. 35, no. 6, pp. 631-638. https://doi.org/10.1007/s10735-004-2190-x
Garagna, Silvia ; Merico, Valeria ; Sebastiano, Vittorio ; Monti, Manuela ; Orlandini, Guido ; Gatti, Rita ; Scandroglio, Renato ; Redi, Carlo Alberto ; Zuccotti, Maurizio. / Three-dimensional localization and dynamics of centromeres in mouse oocytes during folliculogenesis. In: Journal of Molecular Histology. 2004 ; Vol. 35, No. 6. pp. 631-638.
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