Abstract
Very little is known about oocyte nuclear architecture during folliculogenesis. Using antibodies to reveal centromeres, Hoechst-staining to detect the AT-rich pericentromeric heterochromatin (chromocenters), combined with confocal microscopy for the three-dimensional analysis of the nucleus, we demonstrate that during mouse folliculogenesis the oocyte nuclear architecture undergoes dynamic changes. In oocytes isolated from primordial and primary follicles, centromeres and chromocenters were preferentially located at the periphery of the nucleus. During oocyte growth, centromeres and chromocenters were initially found spread within the nucleus and then progressively clustered around the periphery of the nucleolus. Our results indicate that the oocyte nuclear achitecture is developmentally regulated and they contribute to a further understanding of the role of nuclear organization in the regulation of genome functioning during differentiation and development.
| Original language | English |
|---|---|
| Pages (from-to) | 631-638 |
| Number of pages | 8 |
| Journal | Journal of Molecular Histology |
| Volume | 35 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Aug 2004 |
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ASJC Scopus subject areas
- Cell Biology
- Anatomy
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Three-dimensional localization and dynamics of centromeres in mouse oocytes during folliculogenesis. / Garagna, Silvia; Merico, Valeria; Sebastiano, Vittorio; Monti, Manuela; Orlandini, Guido; Gatti, Rita; Scandroglio, Renato; Redi, Carlo Alberto; Zuccotti, Maurizio.
In: Journal of Molecular Histology, Vol. 35, No. 6, 08.2004, p. 631-638.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Three-dimensional localization and dynamics of centromeres in mouse oocytes during folliculogenesis
AU - Garagna, Silvia
AU - Merico, Valeria
AU - Sebastiano, Vittorio
AU - Monti, Manuela
AU - Orlandini, Guido
AU - Gatti, Rita
AU - Scandroglio, Renato
AU - Redi, Carlo Alberto
AU - Zuccotti, Maurizio
PY - 2004/8
Y1 - 2004/8
N2 - Very little is known about oocyte nuclear architecture during folliculogenesis. Using antibodies to reveal centromeres, Hoechst-staining to detect the AT-rich pericentromeric heterochromatin (chromocenters), combined with confocal microscopy for the three-dimensional analysis of the nucleus, we demonstrate that during mouse folliculogenesis the oocyte nuclear architecture undergoes dynamic changes. In oocytes isolated from primordial and primary follicles, centromeres and chromocenters were preferentially located at the periphery of the nucleus. During oocyte growth, centromeres and chromocenters were initially found spread within the nucleus and then progressively clustered around the periphery of the nucleolus. Our results indicate that the oocyte nuclear achitecture is developmentally regulated and they contribute to a further understanding of the role of nuclear organization in the regulation of genome functioning during differentiation and development.
AB - Very little is known about oocyte nuclear architecture during folliculogenesis. Using antibodies to reveal centromeres, Hoechst-staining to detect the AT-rich pericentromeric heterochromatin (chromocenters), combined with confocal microscopy for the three-dimensional analysis of the nucleus, we demonstrate that during mouse folliculogenesis the oocyte nuclear architecture undergoes dynamic changes. In oocytes isolated from primordial and primary follicles, centromeres and chromocenters were preferentially located at the periphery of the nucleus. During oocyte growth, centromeres and chromocenters were initially found spread within the nucleus and then progressively clustered around the periphery of the nucleolus. Our results indicate that the oocyte nuclear achitecture is developmentally regulated and they contribute to a further understanding of the role of nuclear organization in the regulation of genome functioning during differentiation and development.
UR - http://www.scopus.com/inward/record.url?scp=16644403690&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=16644403690&partnerID=8YFLogxK
U2 - 10.1007/s10735-004-2190-x
DO - 10.1007/s10735-004-2190-x
M3 - Article
C2 - 15614617
AN - SCOPUS:16644403690
VL - 35
SP - 631
EP - 638
JO - Journal of Molecular Histology
JF - Journal of Molecular Histology
SN - 1567-2379
IS - 6
ER -