Three-dimensional perfusion culture of human bone marrow cells and generation of osteoinductive grafts

Alessandra Braccini, David Wendt, Claude Jaquiery, Marcel Jakob, Michael Heberer, Linda Kenins, Aleksandra Wodnar-Filipowicz, Rodolfo Quarto, Ivan Martin

Research output: Contribution to journalArticlepeer-review


Three-dimensional (3D) culture systems are critical to investigate cell physiology and to engineer tissue grafts. In this study, we describe a simple yet innovative bioreactor-based approach to seed, expand, and differentiate bone marrow stromal cells (BMSCs) directly in a 3D environment, bypassing the conventional process of monolayer (two-dimensional [2D]) expansion. The system, based on the perfusion of bone marrow-nucleated cells through porous 3D scaffolds, supported the formation of stromal-like tissues, where BMSCs could be cocultured with hematopoietic progenitor cells in proportions dependent on the specific medium supplements. The resulting engineered constructs, when implanted ectopically in nude mice, generated bone tissue more reproducibly, uniformly, and extensively than scaffolds loaded with 2D-expanded BMSCs. The developed system may thus be used as a 3D in vitro model of bone marrow to study interactions between BMSCs and hematopoietic cells as well as to streamline manufacture of osteoinductive grafts in the context of regenerative medicine.

Original languageEnglish
Pages (from-to)1066-1072
Number of pages7
JournalStem Cells
Issue number8
Publication statusPublished - Sep 2005


  • Bone marrow cells
  • Bone marrow stromal cells
  • Colony formation
  • Expansion hematopoiesis
  • Mesenchymal stem cells
  • Osteoprogenitor
  • Tissue regeneration

ASJC Scopus subject areas

  • Cell Biology


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