Three new class I HLA alleles: structure of mRNAs and alternative mechanisms of processing

Luciano Cianetti, Ugo Testa, Luigi Scotto, Roberto La Valle, Antonio Simeone, Giovanni Boccoli, Gianfranco Giannella, Cesare Peschle, Edoardo Boncinelli

Research output: Contribution to journalArticle

Abstract

Sixteen HLA class I clones have been isolated from a SV40-transformed human fibroblast line (GM637) cDNA library. The clones, characterized by hybridization to ABC locus-specific probes and sequence analysis, correspond to transcripts from four different class I genes: A2, A10, Cw4, and Cw6 (or Cw7), as implied by cell typing. Only the A2 sequence was known. The nucleotide and deduced amino acid sequence of the new alleles are reported here, and their structural features are discussed. Two independent cDNAs of A2 specificity display an unusual polyadenylation site located 100 by upstream from the canonical one. Moreover, two cDNAs pertaining to the same C allele display two alternative mechanisms of splicing, which cause either presence or absence in mature transcripts of the transmembrane exon 5 sequence. Transcripts missing this region are predicted to synthesize a nonmembrane-bound, secreted antigen. A soluble protein, specifically reacting with class I-specific HLA antibodies, is found in the supernatant of the GM637 cells. The significance of HLA class I transcripts generated by differential processing is discussed.

Original languageEnglish
Pages (from-to)80-91
Number of pages12
JournalImmunogenetics
Volume29
Issue number2
DOIs
Publication statusPublished - Feb 1989

Fingerprint

varespladib methyl
Alleles
Messenger RNA
Complementary DNA
Clone Cells
MHC Class I Genes
Polyadenylation
antineoplaston A10
Alternative Splicing
Gene Library
Sequence Analysis
Amino Acid Sequence
Exons
Nucleotides
Fibroblasts
Antigens
Antibodies
Proteins

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Cianetti, L., Testa, U., Scotto, L., La Valle, R., Simeone, A., Boccoli, G., ... Boncinelli, E. (1989). Three new class I HLA alleles: structure of mRNAs and alternative mechanisms of processing. Immunogenetics, 29(2), 80-91. https://doi.org/10.1007/BF00395855

Three new class I HLA alleles : structure of mRNAs and alternative mechanisms of processing. / Cianetti, Luciano; Testa, Ugo; Scotto, Luigi; La Valle, Roberto; Simeone, Antonio; Boccoli, Giovanni; Giannella, Gianfranco; Peschle, Cesare; Boncinelli, Edoardo.

In: Immunogenetics, Vol. 29, No. 2, 02.1989, p. 80-91.

Research output: Contribution to journalArticle

Cianetti, L, Testa, U, Scotto, L, La Valle, R, Simeone, A, Boccoli, G, Giannella, G, Peschle, C & Boncinelli, E 1989, 'Three new class I HLA alleles: structure of mRNAs and alternative mechanisms of processing', Immunogenetics, vol. 29, no. 2, pp. 80-91. https://doi.org/10.1007/BF00395855
Cianetti L, Testa U, Scotto L, La Valle R, Simeone A, Boccoli G et al. Three new class I HLA alleles: structure of mRNAs and alternative mechanisms of processing. Immunogenetics. 1989 Feb;29(2):80-91. https://doi.org/10.1007/BF00395855
Cianetti, Luciano ; Testa, Ugo ; Scotto, Luigi ; La Valle, Roberto ; Simeone, Antonio ; Boccoli, Giovanni ; Giannella, Gianfranco ; Peschle, Cesare ; Boncinelli, Edoardo. / Three new class I HLA alleles : structure of mRNAs and alternative mechanisms of processing. In: Immunogenetics. 1989 ; Vol. 29, No. 2. pp. 80-91.
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