Three new class I HLA alleles: structure of mRNAs and alternative mechanisms of processing

Luciano Cianetti, Ugo Testa, Luigi Scotto, Roberto La Valle, Antonio Simeone, Giovanni Boccoli, Gianfranco Giannella, Cesare Peschle, Edoardo Boncinelli

Research output: Contribution to journalArticlepeer-review

Abstract

Sixteen HLA class I clones have been isolated from a SV40-transformed human fibroblast line (GM637) cDNA library. The clones, characterized by hybridization to ABC locus-specific probes and sequence analysis, correspond to transcripts from four different class I genes: A2, A10, Cw4, and Cw6 (or Cw7), as implied by cell typing. Only the A2 sequence was known. The nucleotide and deduced amino acid sequence of the new alleles are reported here, and their structural features are discussed. Two independent cDNAs of A2 specificity display an unusual polyadenylation site located 100 by upstream from the canonical one. Moreover, two cDNAs pertaining to the same C allele display two alternative mechanisms of splicing, which cause either presence or absence in mature transcripts of the transmembrane exon 5 sequence. Transcripts missing this region are predicted to synthesize a nonmembrane-bound, secreted antigen. A soluble protein, specifically reacting with class I-specific HLA antibodies, is found in the supernatant of the GM637 cells. The significance of HLA class I transcripts generated by differential processing is discussed.

Original languageEnglish
Pages (from-to)80-91
Number of pages12
JournalImmunogenetics
Volume29
Issue number2
DOIs
Publication statusPublished - Feb 1989

ASJC Scopus subject areas

  • Immunology
  • Genetics

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