Abstract
Sixteen HLA class I clones have been isolated from a SV40-transformed human fibroblast line (GM637) cDNA library. The clones, characterized by hybridization to ABC locus-specific probes and sequence analysis, correspond to transcripts from four different class I genes: A2, A10, Cw4, and Cw6 (or Cw7), as implied by cell typing. Only the A2 sequence was known. The nucleotide and deduced amino acid sequence of the new alleles are reported here, and their structural features are discussed. Two independent cDNAs of A2 specificity display an unusual polyadenylation site located 100 by upstream from the canonical one. Moreover, two cDNAs pertaining to the same C allele display two alternative mechanisms of splicing, which cause either presence or absence in mature transcripts of the transmembrane exon 5 sequence. Transcripts missing this region are predicted to synthesize a nonmembrane-bound, secreted antigen. A soluble protein, specifically reacting with class I-specific HLA antibodies, is found in the supernatant of the GM637 cells. The significance of HLA class I transcripts generated by differential processing is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 80-91 |
| Number of pages | 12 |
| Journal | Immunogenetics |
| Volume | 29 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1989 |
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ASJC Scopus subject areas
- Immunology
- Genetics
Cite this
Three new class I HLA alleles : structure of mRNAs and alternative mechanisms of processing. / Cianetti, Luciano; Testa, Ugo; Scotto, Luigi; La Valle, Roberto; Simeone, Antonio; Boccoli, Giovanni; Giannella, Gianfranco; Peschle, Cesare; Boncinelli, Edoardo.
In: Immunogenetics, Vol. 29, No. 2, 02.1989, p. 80-91.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Three new class I HLA alleles
T2 - structure of mRNAs and alternative mechanisms of processing
AU - Cianetti, Luciano
AU - Testa, Ugo
AU - Scotto, Luigi
AU - La Valle, Roberto
AU - Simeone, Antonio
AU - Boccoli, Giovanni
AU - Giannella, Gianfranco
AU - Peschle, Cesare
AU - Boncinelli, Edoardo
PY - 1989/2
Y1 - 1989/2
N2 - Sixteen HLA class I clones have been isolated from a SV40-transformed human fibroblast line (GM637) cDNA library. The clones, characterized by hybridization to ABC locus-specific probes and sequence analysis, correspond to transcripts from four different class I genes: A2, A10, Cw4, and Cw6 (or Cw7), as implied by cell typing. Only the A2 sequence was known. The nucleotide and deduced amino acid sequence of the new alleles are reported here, and their structural features are discussed. Two independent cDNAs of A2 specificity display an unusual polyadenylation site located 100 by upstream from the canonical one. Moreover, two cDNAs pertaining to the same C allele display two alternative mechanisms of splicing, which cause either presence or absence in mature transcripts of the transmembrane exon 5 sequence. Transcripts missing this region are predicted to synthesize a nonmembrane-bound, secreted antigen. A soluble protein, specifically reacting with class I-specific HLA antibodies, is found in the supernatant of the GM637 cells. The significance of HLA class I transcripts generated by differential processing is discussed.
AB - Sixteen HLA class I clones have been isolated from a SV40-transformed human fibroblast line (GM637) cDNA library. The clones, characterized by hybridization to ABC locus-specific probes and sequence analysis, correspond to transcripts from four different class I genes: A2, A10, Cw4, and Cw6 (or Cw7), as implied by cell typing. Only the A2 sequence was known. The nucleotide and deduced amino acid sequence of the new alleles are reported here, and their structural features are discussed. Two independent cDNAs of A2 specificity display an unusual polyadenylation site located 100 by upstream from the canonical one. Moreover, two cDNAs pertaining to the same C allele display two alternative mechanisms of splicing, which cause either presence or absence in mature transcripts of the transmembrane exon 5 sequence. Transcripts missing this region are predicted to synthesize a nonmembrane-bound, secreted antigen. A soluble protein, specifically reacting with class I-specific HLA antibodies, is found in the supernatant of the GM637 cells. The significance of HLA class I transcripts generated by differential processing is discussed.
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U2 - 10.1007/BF00395855
DO - 10.1007/BF00395855
M3 - Article
C2 - 2914713
AN - SCOPUS:0024492717
VL - 29
SP - 80
EP - 91
JO - Immunogenetics
JF - Immunogenetics
SN - 0093-7711
IS - 2
ER -