Three-year longitudinal study of genotypes of Mycobacterium tuberculosis isolates in Tuscany, Italy

Nicoletta Lari, Laura Rindi, Daniela Bonanni, Nalin Rastogi, Christophe Sola, Enrico Tortoli, Carlo Garzelli

Research output: Contribution to journalArticle

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Abstract

The genetic diversity of 829 strains of Mycobacterium tuberculosis isolated during a 3-year period in Tuscany, Italy, a country with a low prevalence of tuberculosis, from 480 Italian-born and 349 foreign-born patients was determined by spoligotyping. The predominant spoligotype families were T (30.2% of isolates), Haarlem (19.9%), and the Latino-American and Mediterranean family (LAM) (11.2%); the remaining isolates were distributed among the Beijing (6.5%), S (4.2%), East Africa-India (EAI) (3.0%), Bovis (2.3%), Central Asia (CAS) (2.1%), Africanum (1.3%), and X (1.2%) families or were undefined (2.7%) or orphan (14.1%) isolates. Isolates of the families T, Haarlem, Bovis, and X were distributed among Italian- and foreign-born patients almost proportionally to the patients' numbers. Isolates of the LAM family were prevalent in foreign-born people (13.5%, versus 9.6% in Italian-born patients). Isolates of the S family were found almost exclusively in Italian-born patients, while strains of families EAI and CAS were isolated almost exclusively from foreign-born patients; Africanum isolates were all from African-born patients. The isolates of the Beijing family showed a trend to a steady increase during the survey. The prevalence of Beijing strains was 11.7% among foreign-born people and 2.7% among Italian-born patients. The Beijing strains were typed by the standardized IS6110 restriction fragment length polymorphism assay, which yielded a total of 38 distinct IS6110 patterns; 21 isolates (39.6%) occurred in six distinct clusters; of these, three contained two isolates and the other three contained four, five and six isolates, thus demonstrating that Beijing strains caused several tuberculosis outbreaks in the region. These findings indicate that transmission of Beijing strains between immigrants and the autochthonous population has occurred frequently and suggests an ongoing active transmission of the Beijing genotype in the region.

Original languageEnglish
Pages (from-to)1851-1857
Number of pages7
JournalJournal of Clinical Microbiology
Volume45
Issue number6
DOIs
Publication statusPublished - Jun 2007

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Mycobacterium tuberculosis
Italy
Longitudinal Studies
Genotype
Central Asia
Eastern Africa
Hispanic Americans
India
Tuberculosis
Orphaned Children
Beijing
Restriction Fragment Length Polymorphisms
Disease Outbreaks
Population

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Lari, N., Rindi, L., Bonanni, D., Rastogi, N., Sola, C., Tortoli, E., & Garzelli, C. (2007). Three-year longitudinal study of genotypes of Mycobacterium tuberculosis isolates in Tuscany, Italy. Journal of Clinical Microbiology, 45(6), 1851-1857. https://doi.org/10.1128/JCM.00170-07

Three-year longitudinal study of genotypes of Mycobacterium tuberculosis isolates in Tuscany, Italy. / Lari, Nicoletta; Rindi, Laura; Bonanni, Daniela; Rastogi, Nalin; Sola, Christophe; Tortoli, Enrico; Garzelli, Carlo.

In: Journal of Clinical Microbiology, Vol. 45, No. 6, 06.2007, p. 1851-1857.

Research output: Contribution to journalArticle

Lari, N, Rindi, L, Bonanni, D, Rastogi, N, Sola, C, Tortoli, E & Garzelli, C 2007, 'Three-year longitudinal study of genotypes of Mycobacterium tuberculosis isolates in Tuscany, Italy', Journal of Clinical Microbiology, vol. 45, no. 6, pp. 1851-1857. https://doi.org/10.1128/JCM.00170-07
Lari, Nicoletta ; Rindi, Laura ; Bonanni, Daniela ; Rastogi, Nalin ; Sola, Christophe ; Tortoli, Enrico ; Garzelli, Carlo. / Three-year longitudinal study of genotypes of Mycobacterium tuberculosis isolates in Tuscany, Italy. In: Journal of Clinical Microbiology. 2007 ; Vol. 45, No. 6. pp. 1851-1857.
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abstract = "The genetic diversity of 829 strains of Mycobacterium tuberculosis isolated during a 3-year period in Tuscany, Italy, a country with a low prevalence of tuberculosis, from 480 Italian-born and 349 foreign-born patients was determined by spoligotyping. The predominant spoligotype families were T (30.2{\%} of isolates), Haarlem (19.9{\%}), and the Latino-American and Mediterranean family (LAM) (11.2{\%}); the remaining isolates were distributed among the Beijing (6.5{\%}), S (4.2{\%}), East Africa-India (EAI) (3.0{\%}), Bovis (2.3{\%}), Central Asia (CAS) (2.1{\%}), Africanum (1.3{\%}), and X (1.2{\%}) families or were undefined (2.7{\%}) or orphan (14.1{\%}) isolates. Isolates of the families T, Haarlem, Bovis, and X were distributed among Italian- and foreign-born patients almost proportionally to the patients' numbers. Isolates of the LAM family were prevalent in foreign-born people (13.5{\%}, versus 9.6{\%} in Italian-born patients). Isolates of the S family were found almost exclusively in Italian-born patients, while strains of families EAI and CAS were isolated almost exclusively from foreign-born patients; Africanum isolates were all from African-born patients. The isolates of the Beijing family showed a trend to a steady increase during the survey. The prevalence of Beijing strains was 11.7{\%} among foreign-born people and 2.7{\%} among Italian-born patients. The Beijing strains were typed by the standardized IS6110 restriction fragment length polymorphism assay, which yielded a total of 38 distinct IS6110 patterns; 21 isolates (39.6{\%}) occurred in six distinct clusters; of these, three contained two isolates and the other three contained four, five and six isolates, thus demonstrating that Beijing strains caused several tuberculosis outbreaks in the region. These findings indicate that transmission of Beijing strains between immigrants and the autochthonous population has occurred frequently and suggests an ongoing active transmission of the Beijing genotype in the region.",
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