Threonine aldolase and alanine racemase: Novel examples of convergent evolution in the superfamily of vitamin B6-dependent enzymes

Alessandro Paiardini, Roberto Contestabile, Simona D'Aguanno, Stefano Pascarella, Francesco Bossa

Research output: Contribution to journalArticle

Abstract

Vitamin B6-dependent enzymes may be grouped into five evolutionarily unrelated families, each having a different fold. Within fold type I enzymes, L-threonine aldolase (L-TA) and fungal alanine racemase (AlaRac) belong to a subgroup of structurally and mechanistically closely related proteins, which specialised during evolution to perform different functions. In a previous study, a comparison of the catalytic properties and active site structures of these enzymes suggested that they have a catalytic apparatus with the same basic features. Recently, recombinant D-threonine aldolases (D-TAs) from two bacterial organisms have been characterised, their predicted amino acid sequences showing no significant similarities to any of the known B 6 enzymes. In the present work, a comparative structural analysis suggests that D-TA has an α/β barrel fold and therefore is a fold type III B6 enzyme, as eukaryotic ornithine decarboxylase (ODC) and bacterial AlaRac. The presence of both TA and AlaRac in two distinct evolutionary unrelated families represents a novel and interesting example of convergent evolution. The independent emergence of the same catalytic properties in families characterised by completely different folds may have not been determined by chance, but by the similar structural features required to catalyse pyridoxal phosphate-dependent aldolase and racemase reactions.

Original languageEnglish
Pages (from-to)214-219
Number of pages6
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1647
Issue number1-2
DOIs
Publication statusPublished - Apr 11 2003

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Keywords

  • Alanine racemase
  • Catalytic mechanism
  • Convergent evolution
  • Homology modelling
  • Pyridoxal phosphate
  • Threonine aldolase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics

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