Thromboplastin standards

A. M H P van den Besselaar, V. Chantarangkul, A. Tripodi

Research output: Contribution to journalArticlepeer-review


The Prothrombin Time (PT) test is used for monitoring of treatment with Vitamin K-antagonists (VKA). The result of the PT test should be expressed as the International Normalized Ratio (INR). Calculation of INR is based on the availability of International Standards (IS) for thromboplastin and a calibration model. Calibration of a new PT test system is performed with the appropriate IS and fresh plasma samples of healthy (normal) volunteers and patients treated with VKA. The calibration model is based on the assumption of a linear relationship between the log(PT)'s obtained with the new PT system and the reference IS for both normal and patients' samples. Patients' samples for calibration should be selected by rejecting samples beyond the 1.5-4.5 INR range. Outliers should be rejected defined as points with a perpendicular distance greater than three residual standard deviations from the line of relationship. Selection of patients' samples and rejection of outliers result in a reduction of the between-laboratory variation of calibration. In addition to monitoring of VKA, the PT is used for management of patients with chronic liver disease. Likewise, INRliver should be based on calibration with an IS using samples from patients with chronic liver disease.

Original languageEnglish
Pages (from-to)430-436
Number of pages7
Issue number4
Publication statusPublished - Jul 2010


  • International Normalized Ratio
  • Prothrombin Time
  • Standard
  • Thromboplastin
  • Vitamin K-antagonist

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Immunology and Microbiology(all)
  • Applied Microbiology and Biotechnology
  • Pharmacology


Dive into the research topics of 'Thromboplastin standards'. Together they form a unique fingerprint.

Cite this