TY - JOUR
T1 - Thy 1.2+ leukemia cells eradicated from in vitro leukemia-bone marrow cell mixtures by antibody-toxin conjugates
AU - Colombatti, Marco
AU - Colombatti, Alfonso
AU - Blythman, Hildur E.
AU - Bron, Claude
PY - 1984
Y1 - 1984
N2 - A conjugate constituted by a monoclonal anti-Thy 1.2 antibody chemically coupled to the toxic subunit of ricin was synthesized. The conjugate was specifically cytotoxic for Thy 1.2+ (EL 4) but not for Thy 1.2− (BW5147) thymoma cells. In vitro treatment of bone marrow-leukemia cell mixtures (10:1 and 1:1 bone marrow to EL 4 cell ratios) with anti-Thy 1.2-ricin A chain totally eradicated EL 4 tumor cells, while the number of myeloid colony-forming units and pluripotent colony-forming units developed in vitro and in vivo was unaffected, which suggested that the treatment had no toxic side effects at least on the precursor cells examined.
AB - A conjugate constituted by a monoclonal anti-Thy 1.2 antibody chemically coupled to the toxic subunit of ricin was synthesized. The conjugate was specifically cytotoxic for Thy 1.2+ (EL 4) but not for Thy 1.2− (BW5147) thymoma cells. In vitro treatment of bone marrow-leukemia cell mixtures (10:1 and 1:1 bone marrow to EL 4 cell ratios) with anti-Thy 1.2-ricin A chain totally eradicated EL 4 tumor cells, while the number of myeloid colony-forming units and pluripotent colony-forming units developed in vitro and in vivo was unaffected, which suggested that the treatment had no toxic side effects at least on the precursor cells examined.
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U2 - 10.1093/jnci/72.5.1095
DO - 10.1093/jnci/72.5.1095
M3 - Article
C2 - 6585586
AN - SCOPUS:0021269174
VL - 72
SP - 1095
EP - 1099
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
SN - 0027-8874
IS - 5
ER -