Thy 1.2+ leukemia cells eradicated from in vitro leukemia-bone marrow cell mixtures by antibody-toxin conjugates

Marco Colombatti, Alfonso Colombatti, Hildur E. Blythman, Claude Bron

Research output: Contribution to journalArticle

Abstract

A conjugate constituted by a monoclonal anti-Thy 1.2 antibody chemically coupled to the toxic subunit of ricin was synthesized. The conjugate was specifically cytotoxic for Thy 1.2+ (EL 4) but not for Thy 1.2− (BW5147) thymoma cells. In vitro treatment of bone marrow-leukemia cell mixtures (10:1 and 1:1 bone marrow to EL 4 cell ratios) with anti-Thy 1.2-ricin A chain totally eradicated EL 4 tumor cells, while the number of myeloid colony-forming units and pluripotent colony-forming units developed in vitro and in vivo was unaffected, which suggested that the treatment had no toxic side effects at least on the precursor cells examined.

Original languageEnglish
Pages (from-to)1095-1099
Number of pages5
JournalJournal of the National Cancer Institute
Volume72
Issue number5
DOIs
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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