Abstract
A conjugate constituted by a monoclonal anti-Thy 1.2 antibody chemically coupled to the toxic subunit of ricin was synthesized. The conjugate was specifically cytotoxic for Thy 1.2+ (EL 4) but not for Thy 1.2− (BW5147) thymoma cells. In vitro treatment of bone marrow-leukemia cell mixtures (10:1 and 1:1 bone marrow to EL 4 cell ratios) with anti-Thy 1.2-ricin A chain totally eradicated EL 4 tumor cells, while the number of myeloid colony-forming units and pluripotent colony-forming units developed in vitro and in vivo was unaffected, which suggested that the treatment had no toxic side effects at least on the precursor cells examined.
| Original language | English |
|---|---|
| Pages (from-to) | 1095-1099 |
| Number of pages | 5 |
| Journal | Journal of the National Cancer Institute |
| Volume | 72 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1984 |
ASJC Scopus subject areas
- Cancer Research
- Oncology
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Colombatti, M., Colombatti, A., Blythman, H. E., & Bron, C. (1984). Thy 1.2+ leukemia cells eradicated from in vitro leukemia-bone marrow cell mixtures by antibody-toxin conjugates. Journal of the National Cancer Institute, 72(5), 1095-1099. https://doi.org/10.1093/jnci/72.5.1095