Thymomodulin increases HLA-DR expression by macrophages but not T-lymphocyte proliferation in autologous mixed leucocyte reaction

B. Balbi, M. T. Valle, S. Oddera, F. Manca, G. A. Rossi, L. Allegra

Research output: Contribution to journalArticlepeer-review

Abstract

Thymomodulin (TMD), a thymic biological response modifier, stimulates the release of tumour necrosis factor (TNF) and granulocyte-macrophage-colony stimulating factor (GM-CSF) in macrophage-lymphocyte cultures. We investigated the effects of the cytokines released in cultures with TMD, on the expression of human leucocyte antigen-DR (HLA-DR) antigens by alveolar macrophages (AM) and the T-cell proliferation induced in autologous mixed leucocyte reaction (AMLR) cultures or by T-cell mitogens. Among freshly isolated AM, 84 ± 4% were HLA-DR positive, and this proportion was significantly reduced after 24 h cultures (60 ± 3%, p <0.05). In cultures without peripheral blood (PBL) lymphocytes, TMD did not change HLA-DR expression by AM CHLA-DR + AM; whilst in the presence of autologous PBL lymphocytes, TMD induced an increase in the proportions of HLA-DR + AM (TMD 100 μg · ml-1 79 + 3%, p <0.04 vs control cultures). However, TMD did not change the ability of AM to induce T-cell proliferation in AMLR between AM and PBL lymphocytes. In contrast, in PBL mononuclear cell cultures, TMD induced a further increase of the cell proliferation due to the T-cell mitogens interleukin-2 (IL-2) or phytohaemagglutinin (PHA) (p <0.05 vs each control culture with mitogens) or anti-CD3 antibodies (p <0.03 vs control cultures). Thus, the cytokines released in cultures with TMD enhance macrophage HLA-DR expression. Whilst this phenomenon is not associated with changes in the ability of AM to stimulate T-cell proliferation, TMD is able to increase the mitogen-induced T-cell proliferation.

Original languageEnglish
Pages (from-to)102-109
Number of pages8
JournalEuropean Respiratory Journal
Volume6
Issue number1
Publication statusPublished - 1993

Keywords

  • alveolar macrophages
  • autologous mixed leucocyte reaction
  • granulocyte-macrophage-colony stimulating factor
  • human leucocyte antigen DR
  • T-lymphocytes
  • thymomodulin
  • tumour necrosis factor

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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