Thymomodulin increases HLA-DR expression by macrophages but not T-lymphocyte proliferation in autologous mixed leucocyte reaction

B. Balbi, M. T. Valle, S. Oddera, F. Manca, G. A. Rossi, L. Allegra

Research output: Contribution to journalArticle

Abstract

Thymomodulin (TMD), a thymic biological response modifier, stimulates the release of tumour necrosis factor (TNF) and granulocyte-macrophage-colony stimulating factor (GM-CSF) in macrophage-lymphocyte cultures. We investigated the effects of the cytokines released in cultures with TMD, on the expression of human leucocyte antigen-DR (HLA-DR) antigens by alveolar macrophages (AM) and the T-cell proliferation induced in autologous mixed leucocyte reaction (AMLR) cultures or by T-cell mitogens. Among freshly isolated AM, 84 ± 4% were HLA-DR positive, and this proportion was significantly reduced after 24 h cultures (60 ± 3%, p <0.05). In cultures without peripheral blood (PBL) lymphocytes, TMD did not change HLA-DR expression by AM CHLA-DR + AM; whilst in the presence of autologous PBL lymphocytes, TMD induced an increase in the proportions of HLA-DR + AM (TMD 100 μg · ml-1 79 + 3%, p <0.04 vs control cultures). However, TMD did not change the ability of AM to induce T-cell proliferation in AMLR between AM and PBL lymphocytes. In contrast, in PBL mononuclear cell cultures, TMD induced a further increase of the cell proliferation due to the T-cell mitogens interleukin-2 (IL-2) or phytohaemagglutinin (PHA) (p <0.05 vs each control culture with mitogens) or anti-CD3 antibodies (p <0.03 vs control cultures). Thus, the cytokines released in cultures with TMD enhance macrophage HLA-DR expression. Whilst this phenomenon is not associated with changes in the ability of AM to stimulate T-cell proliferation, TMD is able to increase the mitogen-induced T-cell proliferation.

Original languageEnglish
Pages (from-to)102-109
Number of pages8
JournalEuropean Respiratory Journal
Volume6
Issue number1
Publication statusPublished - 1993

Fingerprint

Mixed Lymphocyte Culture Test
HLA Antigens
Alveolar Macrophages
Macrophages
T-Lymphocytes
Mitogens
Cell Proliferation
Lymphocytes
Cytokines
thymomodulin
Immunologic Factors
Phytohemagglutinins
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-2
Anti-Idiotypic Antibodies
Blood Cells
Cell Culture Techniques
Tumor Necrosis Factor-alpha
Antigens

Keywords

  • alveolar macrophages
  • autologous mixed leucocyte reaction
  • granulocyte-macrophage-colony stimulating factor
  • human leucocyte antigen DR
  • T-lymphocytes
  • thymomodulin
  • tumour necrosis factor

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Thymomodulin increases HLA-DR expression by macrophages but not T-lymphocyte proliferation in autologous mixed leucocyte reaction. / Balbi, B.; Valle, M. T.; Oddera, S.; Manca, F.; Rossi, G. A.; Allegra, L.

In: European Respiratory Journal, Vol. 6, No. 1, 1993, p. 102-109.

Research output: Contribution to journalArticle

Balbi, B. ; Valle, M. T. ; Oddera, S. ; Manca, F. ; Rossi, G. A. ; Allegra, L. / Thymomodulin increases HLA-DR expression by macrophages but not T-lymphocyte proliferation in autologous mixed leucocyte reaction. In: European Respiratory Journal. 1993 ; Vol. 6, No. 1. pp. 102-109.
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abstract = "Thymomodulin (TMD), a thymic biological response modifier, stimulates the release of tumour necrosis factor (TNF) and granulocyte-macrophage-colony stimulating factor (GM-CSF) in macrophage-lymphocyte cultures. We investigated the effects of the cytokines released in cultures with TMD, on the expression of human leucocyte antigen-DR (HLA-DR) antigens by alveolar macrophages (AM) and the T-cell proliferation induced in autologous mixed leucocyte reaction (AMLR) cultures or by T-cell mitogens. Among freshly isolated AM, 84 ± 4{\%} were HLA-DR positive, and this proportion was significantly reduced after 24 h cultures (60 ± 3{\%}, p <0.05). In cultures without peripheral blood (PBL) lymphocytes, TMD did not change HLA-DR expression by AM CHLA-DR + AM; whilst in the presence of autologous PBL lymphocytes, TMD induced an increase in the proportions of HLA-DR + AM (TMD 100 μg · ml-1 79 + 3{\%}, p <0.04 vs control cultures). However, TMD did not change the ability of AM to induce T-cell proliferation in AMLR between AM and PBL lymphocytes. In contrast, in PBL mononuclear cell cultures, TMD induced a further increase of the cell proliferation due to the T-cell mitogens interleukin-2 (IL-2) or phytohaemagglutinin (PHA) (p <0.05 vs each control culture with mitogens) or anti-CD3 antibodies (p <0.03 vs control cultures). Thus, the cytokines released in cultures with TMD enhance macrophage HLA-DR expression. Whilst this phenomenon is not associated with changes in the ability of AM to stimulate T-cell proliferation, TMD is able to increase the mitogen-induced T-cell proliferation.",
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AU - Balbi, B.

AU - Valle, M. T.

AU - Oddera, S.

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AU - Rossi, G. A.

AU - Allegra, L.

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N2 - Thymomodulin (TMD), a thymic biological response modifier, stimulates the release of tumour necrosis factor (TNF) and granulocyte-macrophage-colony stimulating factor (GM-CSF) in macrophage-lymphocyte cultures. We investigated the effects of the cytokines released in cultures with TMD, on the expression of human leucocyte antigen-DR (HLA-DR) antigens by alveolar macrophages (AM) and the T-cell proliferation induced in autologous mixed leucocyte reaction (AMLR) cultures or by T-cell mitogens. Among freshly isolated AM, 84 ± 4% were HLA-DR positive, and this proportion was significantly reduced after 24 h cultures (60 ± 3%, p <0.05). In cultures without peripheral blood (PBL) lymphocytes, TMD did not change HLA-DR expression by AM CHLA-DR + AM; whilst in the presence of autologous PBL lymphocytes, TMD induced an increase in the proportions of HLA-DR + AM (TMD 100 μg · ml-1 79 + 3%, p <0.04 vs control cultures). However, TMD did not change the ability of AM to induce T-cell proliferation in AMLR between AM and PBL lymphocytes. In contrast, in PBL mononuclear cell cultures, TMD induced a further increase of the cell proliferation due to the T-cell mitogens interleukin-2 (IL-2) or phytohaemagglutinin (PHA) (p <0.05 vs each control culture with mitogens) or anti-CD3 antibodies (p <0.03 vs control cultures). Thus, the cytokines released in cultures with TMD enhance macrophage HLA-DR expression. Whilst this phenomenon is not associated with changes in the ability of AM to stimulate T-cell proliferation, TMD is able to increase the mitogen-induced T-cell proliferation.

AB - Thymomodulin (TMD), a thymic biological response modifier, stimulates the release of tumour necrosis factor (TNF) and granulocyte-macrophage-colony stimulating factor (GM-CSF) in macrophage-lymphocyte cultures. We investigated the effects of the cytokines released in cultures with TMD, on the expression of human leucocyte antigen-DR (HLA-DR) antigens by alveolar macrophages (AM) and the T-cell proliferation induced in autologous mixed leucocyte reaction (AMLR) cultures or by T-cell mitogens. Among freshly isolated AM, 84 ± 4% were HLA-DR positive, and this proportion was significantly reduced after 24 h cultures (60 ± 3%, p <0.05). In cultures without peripheral blood (PBL) lymphocytes, TMD did not change HLA-DR expression by AM CHLA-DR + AM; whilst in the presence of autologous PBL lymphocytes, TMD induced an increase in the proportions of HLA-DR + AM (TMD 100 μg · ml-1 79 + 3%, p <0.04 vs control cultures). However, TMD did not change the ability of AM to induce T-cell proliferation in AMLR between AM and PBL lymphocytes. In contrast, in PBL mononuclear cell cultures, TMD induced a further increase of the cell proliferation due to the T-cell mitogens interleukin-2 (IL-2) or phytohaemagglutinin (PHA) (p <0.05 vs each control culture with mitogens) or anti-CD3 antibodies (p <0.03 vs control cultures). Thus, the cytokines released in cultures with TMD enhance macrophage HLA-DR expression. Whilst this phenomenon is not associated with changes in the ability of AM to stimulate T-cell proliferation, TMD is able to increase the mitogen-induced T-cell proliferation.

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