Abstract
Thymomodulin (TMD), a thymic biological response modifier, stimulates the release of tumour necrosis factor (TNF) and granulocyte-macrophage-colony stimulating factor (GM-CSF) in macrophage-lymphocyte cultures. We investigated the effects of the cytokines released in cultures with TMD, on the expression of human leucocyte antigen-DR (HLA-DR) antigens by alveolar macrophages (AM) and the T-cell proliferation induced in autologous mixed leucocyte reaction (AMLR) cultures or by T-cell mitogens. Among freshly isolated AM, 84 ± 4% were HLA-DR positive, and this proportion was significantly reduced after 24 h cultures (60 ± 3%, p <0.05). In cultures without peripheral blood (PBL) lymphocytes, TMD did not change HLA-DR expression by AM CHLA-DR + AM; whilst in the presence of autologous PBL lymphocytes, TMD induced an increase in the proportions of HLA-DR + AM (TMD 100 μg · ml-1 79 + 3%, p <0.04 vs control cultures). However, TMD did not change the ability of AM to induce T-cell proliferation in AMLR between AM and PBL lymphocytes. In contrast, in PBL mononuclear cell cultures, TMD induced a further increase of the cell proliferation due to the T-cell mitogens interleukin-2 (IL-2) or phytohaemagglutinin (PHA) (p <0.05 vs each control culture with mitogens) or anti-CD3 antibodies (p <0.03 vs control cultures). Thus, the cytokines released in cultures with TMD enhance macrophage HLA-DR expression. Whilst this phenomenon is not associated with changes in the ability of AM to stimulate T-cell proliferation, TMD is able to increase the mitogen-induced T-cell proliferation.
| Original language | English |
|---|---|
| Pages (from-to) | 102-109 |
| Number of pages | 8 |
| Journal | European Respiratory Journal |
| Volume | 6 |
| Issue number | 1 |
| Publication status | Published - 1993 |
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Keywords
- alveolar macrophages
- autologous mixed leucocyte reaction
- granulocyte-macrophage-colony stimulating factor
- human leucocyte antigen DR
- T-lymphocytes
- thymomodulin
- tumour necrosis factor
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
Cite this
Thymomodulin increases HLA-DR expression by macrophages but not T-lymphocyte proliferation in autologous mixed leucocyte reaction. / Balbi, B.; Valle, M. T.; Oddera, S.; Manca, F.; Rossi, G. A.; Allegra, L.
In: European Respiratory Journal, Vol. 6, No. 1, 1993, p. 102-109.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Thymomodulin increases HLA-DR expression by macrophages but not T-lymphocyte proliferation in autologous mixed leucocyte reaction
AU - Balbi, B.
AU - Valle, M. T.
AU - Oddera, S.
AU - Manca, F.
AU - Rossi, G. A.
AU - Allegra, L.
PY - 1993
Y1 - 1993
N2 - Thymomodulin (TMD), a thymic biological response modifier, stimulates the release of tumour necrosis factor (TNF) and granulocyte-macrophage-colony stimulating factor (GM-CSF) in macrophage-lymphocyte cultures. We investigated the effects of the cytokines released in cultures with TMD, on the expression of human leucocyte antigen-DR (HLA-DR) antigens by alveolar macrophages (AM) and the T-cell proliferation induced in autologous mixed leucocyte reaction (AMLR) cultures or by T-cell mitogens. Among freshly isolated AM, 84 ± 4% were HLA-DR positive, and this proportion was significantly reduced after 24 h cultures (60 ± 3%, p <0.05). In cultures without peripheral blood (PBL) lymphocytes, TMD did not change HLA-DR expression by AM CHLA-DR + AM; whilst in the presence of autologous PBL lymphocytes, TMD induced an increase in the proportions of HLA-DR + AM (TMD 100 μg · ml-1 79 + 3%, p <0.04 vs control cultures). However, TMD did not change the ability of AM to induce T-cell proliferation in AMLR between AM and PBL lymphocytes. In contrast, in PBL mononuclear cell cultures, TMD induced a further increase of the cell proliferation due to the T-cell mitogens interleukin-2 (IL-2) or phytohaemagglutinin (PHA) (p <0.05 vs each control culture with mitogens) or anti-CD3 antibodies (p <0.03 vs control cultures). Thus, the cytokines released in cultures with TMD enhance macrophage HLA-DR expression. Whilst this phenomenon is not associated with changes in the ability of AM to stimulate T-cell proliferation, TMD is able to increase the mitogen-induced T-cell proliferation.
AB - Thymomodulin (TMD), a thymic biological response modifier, stimulates the release of tumour necrosis factor (TNF) and granulocyte-macrophage-colony stimulating factor (GM-CSF) in macrophage-lymphocyte cultures. We investigated the effects of the cytokines released in cultures with TMD, on the expression of human leucocyte antigen-DR (HLA-DR) antigens by alveolar macrophages (AM) and the T-cell proliferation induced in autologous mixed leucocyte reaction (AMLR) cultures or by T-cell mitogens. Among freshly isolated AM, 84 ± 4% were HLA-DR positive, and this proportion was significantly reduced after 24 h cultures (60 ± 3%, p <0.05). In cultures without peripheral blood (PBL) lymphocytes, TMD did not change HLA-DR expression by AM CHLA-DR + AM; whilst in the presence of autologous PBL lymphocytes, TMD induced an increase in the proportions of HLA-DR + AM (TMD 100 μg · ml-1 79 + 3%, p <0.04 vs control cultures). However, TMD did not change the ability of AM to induce T-cell proliferation in AMLR between AM and PBL lymphocytes. In contrast, in PBL mononuclear cell cultures, TMD induced a further increase of the cell proliferation due to the T-cell mitogens interleukin-2 (IL-2) or phytohaemagglutinin (PHA) (p <0.05 vs each control culture with mitogens) or anti-CD3 antibodies (p <0.03 vs control cultures). Thus, the cytokines released in cultures with TMD enhance macrophage HLA-DR expression. Whilst this phenomenon is not associated with changes in the ability of AM to stimulate T-cell proliferation, TMD is able to increase the mitogen-induced T-cell proliferation.
KW - alveolar macrophages
KW - autologous mixed leucocyte reaction
KW - granulocyte-macrophage-colony stimulating factor
KW - human leucocyte antigen DR
KW - T-lymphocytes
KW - thymomodulin
KW - tumour necrosis factor
UR - http://www.scopus.com/inward/record.url?scp=0027530717&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027530717&partnerID=8YFLogxK
M3 - Article
C2 - 8425578
AN - SCOPUS:0027530717
VL - 6
SP - 102
EP - 109
JO - European Journal of Respiratory Diseases
JF - European Journal of Respiratory Diseases
SN - 0903-1936
IS - 1
ER -