TY - JOUR
T1 - Thymopentin increases the survival of mice after allogeneic blood transfusion, bacterial gavage, and burn injury
AU - Braga, M.
AU - Di Francesco, A.
AU - Gianotti, L.
AU - Vignali, A.
AU - Costantini, E.
AU - Socci, C.
AU - Fortis, C.
AU - Paganelli, G.
AU - Di Carlo, V.
PY - 1994
Y1 - 1994
N2 - Objective: To investigate the effect of blood transfusion on mortality and the incidence of bacterial translocation in mice subjected to thermal burn or bacterial gavage, or both, and to assess the influence of thymopentin on mortality. Design: Randomly controlled experiments. Setting: University departments of surgery, immunology and nuclear medicine. Material: 235 Balb/c (H-2(d)) and C3H/HeJ (H-2(k)) mice. Interventions: 8 groups of 20 mice each received: saline infusion (controls), blood transfusion (BT) alone, 20% burn alone, gavage with 1 x 1010 Escherichia coli alone, BT and gavage, BT and burn, burn and gavage, or BT, burn, and gavage. A further 3 groups of 10 mice were all gavaged with 111In-biotin labelled E coli and randomised to additional BT and burn, BT alone, or burn alone. 98 mice that had had BT, burn, and gavage, were then randomised to receive thymopentin 0, 0.1, 1, or 5 mg/kg/day for 15 days. The impact of the pretreatment with thymopentin on PGE2 concentration was also evaluated in a separate group of 45 mice that received BT, burn, and gavage; or burn and gavage. Main outcome measures: Survival, degree of translocation. Results: The highest mortality (75%) was in the BT, burn, and gavage group. BT alone significantly reduced survival in burned mice, whereas BT alone or associated with gavage had no effect. Thermal injury had the most influence on bacterial translocation, whereas BT did not increase it. Thymopentin significantly improved survival, particularly in the higher doses. The pretreatment with thymopentin significantly reduced PGE2 concentration after BT, burn and gavage. Conclusion: Burn injury significantly increased mortality in the presence of immune deficiency caused by BT. Thymopentin reduced mortality, possibly by immunomodulation.
AB - Objective: To investigate the effect of blood transfusion on mortality and the incidence of bacterial translocation in mice subjected to thermal burn or bacterial gavage, or both, and to assess the influence of thymopentin on mortality. Design: Randomly controlled experiments. Setting: University departments of surgery, immunology and nuclear medicine. Material: 235 Balb/c (H-2(d)) and C3H/HeJ (H-2(k)) mice. Interventions: 8 groups of 20 mice each received: saline infusion (controls), blood transfusion (BT) alone, 20% burn alone, gavage with 1 x 1010 Escherichia coli alone, BT and gavage, BT and burn, burn and gavage, or BT, burn, and gavage. A further 3 groups of 10 mice were all gavaged with 111In-biotin labelled E coli and randomised to additional BT and burn, BT alone, or burn alone. 98 mice that had had BT, burn, and gavage, were then randomised to receive thymopentin 0, 0.1, 1, or 5 mg/kg/day for 15 days. The impact of the pretreatment with thymopentin on PGE2 concentration was also evaluated in a separate group of 45 mice that received BT, burn, and gavage; or burn and gavage. Main outcome measures: Survival, degree of translocation. Results: The highest mortality (75%) was in the BT, burn, and gavage group. BT alone significantly reduced survival in burned mice, whereas BT alone or associated with gavage had no effect. Thermal injury had the most influence on bacterial translocation, whereas BT did not increase it. Thymopentin significantly improved survival, particularly in the higher doses. The pretreatment with thymopentin significantly reduced PGE2 concentration after BT, burn and gavage. Conclusion: Burn injury significantly increased mortality in the presence of immune deficiency caused by BT. Thymopentin reduced mortality, possibly by immunomodulation.
KW - Bacterial translocation
KW - Blood transfusion
KW - Burn injury
KW - prostaglandin E
KW - Thymopentin
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M3 - Article
C2 - 7948352
AN - SCOPUS:0028361672
VL - 160
SP - 345
EP - 350
JO - European Journal of Surgery, Acta Chirurgica
JF - European Journal of Surgery, Acta Chirurgica
SN - 1102-4151
IS - 6-7
ER -