Thymosin α 1 potentiates the release by CD8(+) cells of soluble factors able to inhibit HIV-1 and human T lymphotropic virus 1 infection in vitro

Claudia Matteucci, Antonella Minutolo, Michela Pollicita, Emanuela Balestrieri, Sandro Grelli, Gabriella D'Ettorre, Vincenzo Vullo, Ilaria Bucci, Alessandra Luchini, Stefano Aquaro, Paola Sinibaldi-Vallebona, Beatrice Macchi, Carlo F ederico Perno, Antonio Mastino, Enrico Garaci

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Thymosin α-1 (Tα1) exploits a specific action on lymphoid cells and is able to induce in peripheral blood mononuclear cells (PBMCs) a strong transcriptional response. CD8 antiviral factor activity plays a role in the control or prevention of HIV-1 infection by a non-cytolytic mechanism. The ability of Tα1 to modulate the release of antiretroviral soluble factors by CD8(+) cells was investigated.

METHODS: Supernatants from lipopolysaccharide (LPS) stimulated CD8(+)-isolated cells treated with Tα1 were screened on in vitro infection of human monocyte-derived macrophages (MDMs) and PBMCs with HIV-1, and of PBMCs with human T lymphotropic virus 1 (HTLV-1). In CD8(+) cells, as well as in PBMCs of healthy donors as from HIV(+) individuals, a microarray analysis to assess the transcriptional response after treatment was performed.

RESULTS: Tα1 potentiates the release, in LPS-stimulated CD8(+) cells, of soluble factors able to inhibit both in vitro HIV-1 infection of MDMs and PBMCs and in vitro HTLV-1 infection of PBMCs. A distinctive transcriptional profile was induced by Tα1 in PBMCs from HIV(+) donors.

CONCLUSIONS: These findings suggest that Tα1 would represent a re-evaluated approach to antiretroviral therapy in combination with innovative treatments and with vaccine administration.

Original languageEnglish
Pages (from-to)S83-S100
JournalExpert Opinion on Biological Therapy
Volume15
DOIs
Publication statusPublished - 2015

Keywords

  • CD8 antiviral factor
  • chemokines
  • HIV-1
  • human T lymphotropic virus 1
  • immunotherapy
  • Thymosin α 1

ASJC Scopus subject areas

  • Medicine(all)

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