Thymosin β-10 gene overexpression correlated with the highly malignant neoplastic phenotype of transformed thyroid cells in vivo and in vitro

Daniela Califano, Carmen Monaco, Giovanni Santelli, Ada Giuliano, Maria Luisa Veronese, Maria Teresa Berlingieri, Vittorio De Franciscis, Nicole Berger, Francesco Trapasso, Massimo Santoro, Giuseppe Viglietto, Alfredo Fusco

Research output: Contribution to journalArticlepeer-review

Abstract

A subtractive thyroid cDNA library was constructed from two human thyroid carcinoma cell lines originating from an anaplastic carcinoma and a papillary thyroid carcinoma. The library was used to identify genes correlated with the progression to a highly malignant phenotype. The thymosin β-10 gene was isolated and found to be expressed at much higher levels in the anaplastic cell line than in the papillary cells. The thymosin β-10 gene was overexpressed in five carcinoma cell lines compared with normal thyroid tissue and normal thyroid primary culture cells. The highest expression occurred in the most malignant cell lines. Thymosin β-10 gene expression was also increased in surgically removed human thyroid carcinomas and was highest in the anaplastic carcinomas. Thymosin β-10 gene expression was correlated with the degree of the malignant phenotype also in rat thyroid cells transfected with cellular and viral oncogenes of different tumorigenicity. These results show that thymosin β-10 overexpression is a general event of thyroid cell neoplastic transformation and suggest that the gene is involved in the progression of thyroid carcinogenesis. Finally, the thymosin β-10 gene was located on chromosome 2q37 by fluorescence in situ hybridization analysis.

Original languageEnglish
Pages (from-to)823-828
Number of pages6
JournalCancer Research
Volume58
Issue number4
Publication statusPublished - Feb 15 1998

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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