Thyroid hormone regulation of cell migration and oxidative metabolism in polymorphonuclear leukocytes: Clinical evidence in thyroidectomized subjects on thyroxine replacement therapy

Franca Marino, Luigina Guasti, Marco Cosentino, Davide De Piazza, Cinzia Simoni, Eliana Piantanida, Mariagrazia Cimpanelli, Catherine Klersy, Luigi Bartalena, Achille Venco, Sergio Lecchini

Research output: Contribution to journalArticle

Abstract

Migration and superoxide anion (O2-) generation were studied in polymorphonuclear leukocytes (PMNs) from 14 athyreotic patients, previously treated by total thyroidectomy and radioiodine therapy for differentiated thyroid carcinoma, and from age- and sex-matched euthyroid healthy controls. Patients were studied twice: in hypothyroidism (visit 1) and after TSH-suppressive L-T4 replacement therapy (visit 2). Random migration and N-formyl-Met-Leu-Phe (fMLP) 0.1-μM induced chemotaxis were similar in cells from patients at both visit 1 and visit 2 and from healthy controls. On the contrary, resting O2- generation in cells from patients was significantly lower than control values, both at visit 1 and 2. At visit 1, fMLP 0.1 μM-induced O2- generation was significantly lower than control values, while phorbol-myristate acetate (PMA) 100-ng/ml induced O2- generation was similar in cells from patients and from controls. At visit 2 both responses increased, resulting in fMLP-induced O2- generation superimposable to control values and PMA-induced O2- generation significantly higher with respect to both visit 1 and cells from controls. In vitro exposure of PMNs from healthy subjects to L-T4 did not affect O2- generation in resting cells, and significantly increased that induced by fMLP or PMA only at high, supra-physiological concentrations. Neither TSH nor T3 had significant effects at any of the concentrations tested. The present results document the existence of a correlation between thyroid status and oxidative metabolism of human PMNs, which is however unlikely to depend upon a direct action of thyroid hormones on these cells.

Original languageEnglish
Pages (from-to)1071-1077
Number of pages7
JournalLife Sciences
Volume78
Issue number10
DOIs
Publication statusPublished - Feb 2 2006

Fingerprint

Thyroxine
Thyroid Hormones
Metabolism
Cell Movement
Neutrophils
Cells
Tetradecanoylphorbol Acetate
Therapeutics
Thyroidectomy
Chemotaxis
Hypothyroidism
Thyroid Neoplasms
Superoxides
Healthy Volunteers
Thyroid Gland
N-formylmethionylphenylalanine

Keywords

  • Chemotaxis
  • Oxidative metabolism
  • Polymorphonuclear leukocytes
  • T
  • T
  • TSH

ASJC Scopus subject areas

  • Pharmacology

Cite this

Thyroid hormone regulation of cell migration and oxidative metabolism in polymorphonuclear leukocytes : Clinical evidence in thyroidectomized subjects on thyroxine replacement therapy. / Marino, Franca; Guasti, Luigina; Cosentino, Marco; De Piazza, Davide; Simoni, Cinzia; Piantanida, Eliana; Cimpanelli, Mariagrazia; Klersy, Catherine; Bartalena, Luigi; Venco, Achille; Lecchini, Sergio.

In: Life Sciences, Vol. 78, No. 10, 02.02.2006, p. 1071-1077.

Research output: Contribution to journalArticle

Marino, F, Guasti, L, Cosentino, M, De Piazza, D, Simoni, C, Piantanida, E, Cimpanelli, M, Klersy, C, Bartalena, L, Venco, A & Lecchini, S 2006, 'Thyroid hormone regulation of cell migration and oxidative metabolism in polymorphonuclear leukocytes: Clinical evidence in thyroidectomized subjects on thyroxine replacement therapy', Life Sciences, vol. 78, no. 10, pp. 1071-1077. https://doi.org/10.1016/j.lfs.2005.06.016
Marino, Franca ; Guasti, Luigina ; Cosentino, Marco ; De Piazza, Davide ; Simoni, Cinzia ; Piantanida, Eliana ; Cimpanelli, Mariagrazia ; Klersy, Catherine ; Bartalena, Luigi ; Venco, Achille ; Lecchini, Sergio. / Thyroid hormone regulation of cell migration and oxidative metabolism in polymorphonuclear leukocytes : Clinical evidence in thyroidectomized subjects on thyroxine replacement therapy. In: Life Sciences. 2006 ; Vol. 78, No. 10. pp. 1071-1077.
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AU - Simoni, Cinzia

AU - Piantanida, Eliana

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