Thyroid hormone regulation of malic enzyme synthesis. Dual tissue-specific control

B. Dozin, M. A. Magnuson, V. M. Nikodem

Research output: Contribution to journalArticlepeer-review

Abstract

The regulatory mechanism(s) involved in the tissue-specific induction of cytosolic malic enzyme (EC 1.1.1.40) by triiodothyronine (T3) have been investigated in rat liver and heart. In these two tissues, cellular malic enzyme mRNA accumulates to different extents in response to hormonal stimulation (11-16- and 3-4-fold above the respective basal levels) (Dozin, B., Magnuson, M.A., and Nikodem, V.M. (1985) Biochemistry 24, 5581-5586). To gain further insight into this pretranslational control, nuclear in vitro runoff transcription assays were performed and correlated with the levels of malic enzyme RNA sequences in cytoplasm. The data demonstrate that the rate of transcription of the malic enzyme gene is stimulated by T3 to similar extents in the liver and heart (3-4-fold above the basal activity). In liver, T3 also promotes an additional increase in cellular malic enzyme mRNA. This additional effect could be due to a tissue-specific change in the rate of degradation of cytoplasmic mRNA or to an effect on malic enzyme mRNA in the nucleus.

Original languageEnglish
Pages (from-to)10290-10292
Number of pages3
JournalJournal of Biological Chemistry
Volume261
Issue number22
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Biochemistry

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