Thyroid hormones and active calcium transport of inside-out red cell membrane vesicles

Alessandro Rubinacci, Paola Divieti, Stefano Lodigiani, Alessandro De Ponti, Michele Samaja

Research output: Contribution to journalArticle

Abstract

Thyroid hormones may influence the active transport of Ca2+ across the cell membrane. To test the physiologic relevance of this mechanism, we used inside-out human red cell membrane vesicles as a model of the cell membrane Ca2+ pump. We monitored by spectrophotometric methods the kinetics of the uptake of Ca2+ in the presence of 10-5-10-10 m thyroid hormones or their analogues. Vesicles freed of calmodulin and protein inhibitor(s) of the Ca2+ pump were also obtained. The results are as follows: (1) Thyroxine inhibits the active Ca2+ uptake; (2) this effect antagonizes that of soluble calmodulin; and (3) triiodothyronine and other analogues of the thyroid hormones are less active than thyroxine. We conclude that the thyroid hormones may influence cell Ca2+ homeostasis by direct action on the Ca2+ pump.

Original languageEnglish
Pages (from-to)235-240
Number of pages6
JournalBiochemical Medicine and Metabolic Biology
Volume48
Issue number3
DOIs
Publication statusPublished - 1992

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ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

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