Tie2 expressing monocytes in the spleen of patients with primary myelofibrosis

Rita Campanelli, Gabriela Fois, Paolo Catarsi, Valentina Poletto, Laura Villani, Benedetta Gaia Erba, Luigi Maddaluno, Basilio Jemos, Silvia Salmoiraghi, Paola Guglielmelli, Vittorio Abbonante, Christian Andrea Di Buduo, Alessandra Balduini, Alessandra Iurlo, Giovanni Barosi, Vittorio Rosti, Margherita Massa, A. M. Vannucchi, M. Balliu, N. BartalucciC. Bogani, A. Bosi, L. Calabresi, G. Corbizzi Fattori, T. Fanelli, R. Fjerza, F. Gesullo, P. Guglielmelli, C. Mannarelli, L. Merli, A. Pacilli, A. Pancrazzi, C. Paoli, L. Pieri, G. Rotunno, E. Sant'Antonio, G. Barosi, Vittorio Rosti, M. Cazzola, I. Ambaglio, P. Bernasconi, S. Catricalà, C. Elena, E. Fugazza, L. Malcovati, C. Milanesi, D. Pietra, F. Ripamonti, M. Rossi, E. Rumi, AGIMM Investigators

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Primary myelofibrosis (PMF) is a Philadelphia-negative (Ph-) myeloproliferative disorder, showing abnormal CD34+ progenitor cell trafficking, splenomegaly, marrow fibrosis leading to extensive extramedullary haematopoiesis, and abnormal neoangiogenesis in either the bone marrow or the spleen. Monocytes expressing the angiopoietin-2 receptor (Tie2) have been shown to support abnormal angiogenic processes in solid tumors through a paracrine action that takes place in proximity to the vessels. In this study we investigated the frequency of Tie2 expressing monocytes in the spleen tissue samples of patients with PMF, and healthy subjects (CTRLs), and evaluated their possible role in favouring spleen angiogenesis. We show by confocal microscopy that in the spleen tissue of patients with PMF, but not of CTRLs, the most of the CD14+ cells are Tie2+ and are close to vessels; by flow cytometry, we found that Tie2 expressing monocytes were Tie2+CD14lowCD16brightCDL62-CCR2- (TEMs) and their frequency was higher (p = 0.008) in spleen tissue-derived mononuclear cells (MNCs) of patients with PMF than in spleen tissue-derived MNCs from CTRLs undergoing splenectomy for abdominal trauma. By in vitro angiogenesis assay we evidenced that conditioned medium of immunomagnetically selected spleen tissue derived CD14+ cells of patients with PMF induced a denser tube like net than that of CTRLs; in addition, CD14+Tie2+ cells sorted from spleen tissue derived single cell suspension of patients with PMF show a higher expression of genes involved in angiogenesis than that found in CTRLs. Our results document the enrichment of Tie2+ monocytes expressing angiogenic genes in the spleen of patients with PMF, suggesting a role for these cells in starting/maintaining the pathological angiogenesis in this organ.

Original languageEnglish
Article numbere0156990
JournalPLoS One
Volume11
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

Primary Myelofibrosis
monocytes
Monocytes
spleen
Spleen
Tissue
angiogenesis
TIE-2 Receptor
Genes
Cells
Angiopoietin-2
cells
Flow cytometry
Confocal microscopy
Conditioned Culture Medium
Bone Marrow
Pathologic Neovascularization
Extramedullary Hematopoiesis
Tumors
Assays

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tie2 expressing monocytes in the spleen of patients with primary myelofibrosis. / Campanelli, Rita; Fois, Gabriela; Catarsi, Paolo; Poletto, Valentina; Villani, Laura; Erba, Benedetta Gaia; Maddaluno, Luigi; Jemos, Basilio; Salmoiraghi, Silvia; Guglielmelli, Paola; Abbonante, Vittorio; Di Buduo, Christian Andrea; Balduini, Alessandra; Iurlo, Alessandra; Barosi, Giovanni; Rosti, Vittorio; Massa, Margherita; Vannucchi, A. M.; Balliu, M.; Bartalucci, N.; Bogani, C.; Bosi, A.; Calabresi, L.; Corbizzi Fattori, G.; Fanelli, T.; Fjerza, R.; Gesullo, F.; Guglielmelli, P.; Mannarelli, C.; Merli, L.; Pacilli, A.; Pancrazzi, A.; Paoli, C.; Pieri, L.; Rotunno, G.; Sant'Antonio, E.; Barosi, G.; Rosti, Vittorio; Cazzola, M.; Ambaglio, I.; Bernasconi, P.; Catricalà, S.; Elena, C.; Fugazza, E.; Malcovati, L.; Milanesi, C.; Pietra, D.; Ripamonti, F.; Rossi, M.; Rumi, E.; AGIMM Investigators.

In: PLoS One, Vol. 11, No. 6, e0156990, 01.06.2016.

Research output: Contribution to journalArticle

Campanelli, R, Fois, G, Catarsi, P, Poletto, V, Villani, L, Erba, BG, Maddaluno, L, Jemos, B, Salmoiraghi, S, Guglielmelli, P, Abbonante, V, Di Buduo, CA, Balduini, A, Iurlo, A, Barosi, G, Rosti, V, Massa, M, Vannucchi, AM, Balliu, M, Bartalucci, N, Bogani, C, Bosi, A, Calabresi, L, Corbizzi Fattori, G, Fanelli, T, Fjerza, R, Gesullo, F, Guglielmelli, P, Mannarelli, C, Merli, L, Pacilli, A, Pancrazzi, A, Paoli, C, Pieri, L, Rotunno, G, Sant'Antonio, E, Barosi, G, Rosti, V, Cazzola, M, Ambaglio, I, Bernasconi, P, Catricalà, S, Elena, C, Fugazza, E, Malcovati, L, Milanesi, C, Pietra, D, Ripamonti, F, Rossi, M, Rumi, E & AGIMM Investigators 2016, 'Tie2 expressing monocytes in the spleen of patients with primary myelofibrosis', PLoS One, vol. 11, no. 6, e0156990. https://doi.org/10.1371/journal.pone.0156990
Campanelli, Rita ; Fois, Gabriela ; Catarsi, Paolo ; Poletto, Valentina ; Villani, Laura ; Erba, Benedetta Gaia ; Maddaluno, Luigi ; Jemos, Basilio ; Salmoiraghi, Silvia ; Guglielmelli, Paola ; Abbonante, Vittorio ; Di Buduo, Christian Andrea ; Balduini, Alessandra ; Iurlo, Alessandra ; Barosi, Giovanni ; Rosti, Vittorio ; Massa, Margherita ; Vannucchi, A. M. ; Balliu, M. ; Bartalucci, N. ; Bogani, C. ; Bosi, A. ; Calabresi, L. ; Corbizzi Fattori, G. ; Fanelli, T. ; Fjerza, R. ; Gesullo, F. ; Guglielmelli, P. ; Mannarelli, C. ; Merli, L. ; Pacilli, A. ; Pancrazzi, A. ; Paoli, C. ; Pieri, L. ; Rotunno, G. ; Sant'Antonio, E. ; Barosi, G. ; Rosti, Vittorio ; Cazzola, M. ; Ambaglio, I. ; Bernasconi, P. ; Catricalà, S. ; Elena, C. ; Fugazza, E. ; Malcovati, L. ; Milanesi, C. ; Pietra, D. ; Ripamonti, F. ; Rossi, M. ; Rumi, E. ; AGIMM Investigators. / Tie2 expressing monocytes in the spleen of patients with primary myelofibrosis. In: PLoS One. 2016 ; Vol. 11, No. 6.
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abstract = "Primary myelofibrosis (PMF) is a Philadelphia-negative (Ph-) myeloproliferative disorder, showing abnormal CD34+ progenitor cell trafficking, splenomegaly, marrow fibrosis leading to extensive extramedullary haematopoiesis, and abnormal neoangiogenesis in either the bone marrow or the spleen. Monocytes expressing the angiopoietin-2 receptor (Tie2) have been shown to support abnormal angiogenic processes in solid tumors through a paracrine action that takes place in proximity to the vessels. In this study we investigated the frequency of Tie2 expressing monocytes in the spleen tissue samples of patients with PMF, and healthy subjects (CTRLs), and evaluated their possible role in favouring spleen angiogenesis. We show by confocal microscopy that in the spleen tissue of patients with PMF, but not of CTRLs, the most of the CD14+ cells are Tie2+ and are close to vessels; by flow cytometry, we found that Tie2 expressing monocytes were Tie2+CD14lowCD16brightCDL62-CCR2- (TEMs) and their frequency was higher (p = 0.008) in spleen tissue-derived mononuclear cells (MNCs) of patients with PMF than in spleen tissue-derived MNCs from CTRLs undergoing splenectomy for abdominal trauma. By in vitro angiogenesis assay we evidenced that conditioned medium of immunomagnetically selected spleen tissue derived CD14+ cells of patients with PMF induced a denser tube like net than that of CTRLs; in addition, CD14+Tie2+ cells sorted from spleen tissue derived single cell suspension of patients with PMF show a higher expression of genes involved in angiogenesis than that found in CTRLs. Our results document the enrichment of Tie2+ monocytes expressing angiogenic genes in the spleen of patients with PMF, suggesting a role for these cells in starting/maintaining the pathological angiogenesis in this organ.",
author = "Rita Campanelli and Gabriela Fois and Paolo Catarsi and Valentina Poletto and Laura Villani and Erba, {Benedetta Gaia} and Luigi Maddaluno and Basilio Jemos and Silvia Salmoiraghi and Paola Guglielmelli and Vittorio Abbonante and {Di Buduo}, {Christian Andrea} and Alessandra Balduini and Alessandra Iurlo and Giovanni Barosi and Vittorio Rosti and Margherita Massa and Vannucchi, {A. M.} and M. Balliu and N. Bartalucci and C. Bogani and A. Bosi and L. Calabresi and {Corbizzi Fattori}, G. and T. Fanelli and R. Fjerza and F. Gesullo and P. Guglielmelli and C. Mannarelli and L. Merli and A. Pacilli and A. Pancrazzi and C. Paoli and L. Pieri and G. Rotunno and E. Sant'Antonio and G. Barosi and Vittorio Rosti and M. Cazzola and I. Ambaglio and P. Bernasconi and S. Catrical{\`a} and C. Elena and E. Fugazza and L. Malcovati and C. Milanesi and D. Pietra and F. Ripamonti and M. Rossi and E. Rumi and {AGIMM Investigators}",
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T1 - Tie2 expressing monocytes in the spleen of patients with primary myelofibrosis

AU - Campanelli, Rita

AU - Fois, Gabriela

AU - Catarsi, Paolo

AU - Poletto, Valentina

AU - Villani, Laura

AU - Erba, Benedetta Gaia

AU - Maddaluno, Luigi

AU - Jemos, Basilio

AU - Salmoiraghi, Silvia

AU - Guglielmelli, Paola

AU - Abbonante, Vittorio

AU - Di Buduo, Christian Andrea

AU - Balduini, Alessandra

AU - Iurlo, Alessandra

AU - Barosi, Giovanni

AU - Rosti, Vittorio

AU - Massa, Margherita

AU - Vannucchi, A. M.

AU - Balliu, M.

AU - Bartalucci, N.

AU - Bogani, C.

AU - Bosi, A.

AU - Calabresi, L.

AU - Corbizzi Fattori, G.

AU - Fanelli, T.

AU - Fjerza, R.

AU - Gesullo, F.

AU - Guglielmelli, P.

AU - Mannarelli, C.

AU - Merli, L.

AU - Pacilli, A.

AU - Pancrazzi, A.

AU - Paoli, C.

AU - Pieri, L.

AU - Rotunno, G.

AU - Sant'Antonio, E.

AU - Barosi, G.

AU - Rosti, Vittorio

AU - Cazzola, M.

AU - Ambaglio, I.

AU - Bernasconi, P.

AU - Catricalà, S.

AU - Elena, C.

AU - Fugazza, E.

AU - Malcovati, L.

AU - Milanesi, C.

AU - Pietra, D.

AU - Ripamonti, F.

AU - Rossi, M.

AU - Rumi, E.

AU - AGIMM Investigators

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Primary myelofibrosis (PMF) is a Philadelphia-negative (Ph-) myeloproliferative disorder, showing abnormal CD34+ progenitor cell trafficking, splenomegaly, marrow fibrosis leading to extensive extramedullary haematopoiesis, and abnormal neoangiogenesis in either the bone marrow or the spleen. Monocytes expressing the angiopoietin-2 receptor (Tie2) have been shown to support abnormal angiogenic processes in solid tumors through a paracrine action that takes place in proximity to the vessels. In this study we investigated the frequency of Tie2 expressing monocytes in the spleen tissue samples of patients with PMF, and healthy subjects (CTRLs), and evaluated their possible role in favouring spleen angiogenesis. We show by confocal microscopy that in the spleen tissue of patients with PMF, but not of CTRLs, the most of the CD14+ cells are Tie2+ and are close to vessels; by flow cytometry, we found that Tie2 expressing monocytes were Tie2+CD14lowCD16brightCDL62-CCR2- (TEMs) and their frequency was higher (p = 0.008) in spleen tissue-derived mononuclear cells (MNCs) of patients with PMF than in spleen tissue-derived MNCs from CTRLs undergoing splenectomy for abdominal trauma. By in vitro angiogenesis assay we evidenced that conditioned medium of immunomagnetically selected spleen tissue derived CD14+ cells of patients with PMF induced a denser tube like net than that of CTRLs; in addition, CD14+Tie2+ cells sorted from spleen tissue derived single cell suspension of patients with PMF show a higher expression of genes involved in angiogenesis than that found in CTRLs. Our results document the enrichment of Tie2+ monocytes expressing angiogenic genes in the spleen of patients with PMF, suggesting a role for these cells in starting/maintaining the pathological angiogenesis in this organ.

AB - Primary myelofibrosis (PMF) is a Philadelphia-negative (Ph-) myeloproliferative disorder, showing abnormal CD34+ progenitor cell trafficking, splenomegaly, marrow fibrosis leading to extensive extramedullary haematopoiesis, and abnormal neoangiogenesis in either the bone marrow or the spleen. Monocytes expressing the angiopoietin-2 receptor (Tie2) have been shown to support abnormal angiogenic processes in solid tumors through a paracrine action that takes place in proximity to the vessels. In this study we investigated the frequency of Tie2 expressing monocytes in the spleen tissue samples of patients with PMF, and healthy subjects (CTRLs), and evaluated their possible role in favouring spleen angiogenesis. We show by confocal microscopy that in the spleen tissue of patients with PMF, but not of CTRLs, the most of the CD14+ cells are Tie2+ and are close to vessels; by flow cytometry, we found that Tie2 expressing monocytes were Tie2+CD14lowCD16brightCDL62-CCR2- (TEMs) and their frequency was higher (p = 0.008) in spleen tissue-derived mononuclear cells (MNCs) of patients with PMF than in spleen tissue-derived MNCs from CTRLs undergoing splenectomy for abdominal trauma. By in vitro angiogenesis assay we evidenced that conditioned medium of immunomagnetically selected spleen tissue derived CD14+ cells of patients with PMF induced a denser tube like net than that of CTRLs; in addition, CD14+Tie2+ cells sorted from spleen tissue derived single cell suspension of patients with PMF show a higher expression of genes involved in angiogenesis than that found in CTRLs. Our results document the enrichment of Tie2+ monocytes expressing angiogenic genes in the spleen of patients with PMF, suggesting a role for these cells in starting/maintaining the pathological angiogenesis in this organ.

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