Tie2 expressing monocytes in the spleen of patients with primary myelofibrosis

Rita Campanelli, Gabriela Fois, Paolo Catarsi, Valentina Poletto, Laura Villani, Benedetta Gaia Erba, Luigi Maddaluno, Basilio Jemos, Silvia Salmoiraghi, Paola Guglielmelli, Vittorio Abbonante, Christian Andrea Di Buduo, A. Balduini, Alessandra Iurlo, Giovanni Barosi, Vittorio Rosti, Margherita Massa, Alessandro M. Vannucchi, M. Balliu, Niccolò BartalucciCostanza Bogani, Alberto Bosi, L. Calabresi, G. Corbizzi Fattori, Tiziana Fanelli, Rajmonda Fjerza, F. Gesullo, Paola Guglielmelli, C. Mannarelli, L. Merli, Annalisa Pacilli, A. Pancrazzi, Chiara Paoli, Lisa Pieri, G. Rotunno, Emanuela Sant'Antonio, G. Barosi, Vittorio Abbonante, A. Balduini, Elisa Bonetti, R. Campanelli, P. Catarsi, Christian A. Di Buduo, G. Fois, M. Massa, V. Poletto, V. Rosti, L. Villani, Mario Cazzola, Ilaria Ambaglio, Paolo Bernasconi, C. I. Casetti, Silvia Catricala, Chiara Elena, Elena Fugazza, Anna Gallì, Luca Malcovati, Chiara Milanesi, Cristiana Pascutto, Daniela Pietra, Francesco Ripamonti, Marianna Rossi, Elisa Rumi, Elisabetta Dejana, Ferruccio Breviario, Monica Corada, M. Malinverno, Alessandro Rambaldi, G. Chioda, Maria L. Ferrari, Guido Finazzi, Mariachiara Finazzi, Clara Belotti, C. Boroni, S. Salmoiraghi, A. Amaru, Josée Golay, S. Bortoluzzi, Andrea Bisognin, Alessandro Coppe, C. Saccoman, Rossella Manfredini, Lucia Artuso, Isabella Bernardis, Elisa Bianchi, Monica Montanari, Valentina Pennucci, Zelia Prudente, Sebastiano Rontauroli, C. Rossi, Samantha Ruberti, Simona Salati, Enrico Tagliafico, Elena Tenedini, R. Zini

Research output: Contribution to journalArticle

Abstract

Primary myelofibrosis (PMF) is a Philadelphia-negative (Ph-) myeloproliferative disorder, showing abnormal CD34+ progenitor cell trafficking, splenomegaly, marrow fibrosis leading to extensive extramedullary haematopoiesis, and abnormal neoangiogenesis in either the bone marrow or the spleen. Monocytes expressing the angiopoietin-2 receptor (Tie2) have been shown to support abnormal angiogenic processes in solid tumors through a paracrine action that takes place in proximity to the vessels. In this study we investigated the frequency of Tie2 expressing monocytes in the spleen tissue samples of patients with PMF, and healthy subjects (CTRLs), and evaluated their possible role in favouring spleen angiogenesis. We show by confocal microscopy that in the spleen tissue of patients with PMF, but not of CTRLs, the most of the CD14+ cells are Tie2+ and are close to vessels; by flow cytometry, we found that Tie2 expressing monocytes were Tie2+CD14lowCD16brightCDL62-CCR2- (TEMs) and their frequency was higher (p = 0.008) in spleen tissue-derived mononuclear cells (MNCs) of patients with PMF than in spleen tissue-derived MNCs from CTRLs undergoing splenectomy for abdominal trauma. By in vitro angiogenesis assay we evidenced that conditioned medium of immunomagnetically selected spleen tissue derived CD14+ cells of patients with PMF induced a denser tube like net than that of CTRLs; in addition, CD14+Tie2+ cells sorted from spleen tissue derived single cell suspension of patients with PMF show a higher expression of genes involved in angiogenesis than that found in CTRLs. Our results document the enrichment of Tie2+ monocytes expressing angiogenic genes in the spleen of patients with PMF, suggesting a role for these cells in starting/maintaining the pathological angiogenesis in this organ.

Original languageEnglish
Article numbere0156990
JournalPLoS One
Volume11
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

Primary Myelofibrosis
monocytes
Monocytes
spleen
Spleen
Tissue
angiogenesis
TIE-2 Receptor
Genes
Cells
Angiopoietin-2
cells
Flow cytometry
Confocal microscopy
Conditioned Culture Medium
Bone Marrow
Pathologic Neovascularization
Extramedullary Hematopoiesis
Tumors
Assays

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tie2 expressing monocytes in the spleen of patients with primary myelofibrosis. / Campanelli, Rita; Fois, Gabriela; Catarsi, Paolo; Poletto, Valentina; Villani, Laura; Erba, Benedetta Gaia; Maddaluno, Luigi; Jemos, Basilio; Salmoiraghi, Silvia; Guglielmelli, Paola; Abbonante, Vittorio; Di Buduo, Christian Andrea; Balduini, A.; Iurlo, Alessandra; Barosi, Giovanni; Rosti, Vittorio; Massa, Margherita; Vannucchi, Alessandro M.; Balliu, M.; Bartalucci, Niccolò; Bogani, Costanza; Bosi, Alberto; Calabresi, L.; Corbizzi Fattori, G.; Fanelli, Tiziana; Fjerza, Rajmonda; Gesullo, F.; Guglielmelli, Paola; Mannarelli, C.; Merli, L.; Pacilli, Annalisa; Pancrazzi, A.; Paoli, Chiara; Pieri, Lisa; Rotunno, G.; Sant'Antonio, Emanuela; Barosi, G.; Abbonante, Vittorio; Balduini, A.; Bonetti, Elisa; Campanelli, R.; Catarsi, P.; Di Buduo, Christian A.; Fois, G.; Massa, M.; Poletto, V.; Rosti, V.; Villani, L.; Cazzola, Mario; Ambaglio, Ilaria; Bernasconi, Paolo; Casetti, C. I.; Catricala, Silvia; Elena, Chiara; Fugazza, Elena; Gallì, Anna; Malcovati, Luca; Milanesi, Chiara; Pascutto, Cristiana; Pietra, Daniela; Ripamonti, Francesco; Rossi, Marianna; Rumi, Elisa; Dejana, Elisabetta; Breviario, Ferruccio; Corada, Monica; Malinverno, M.; Rambaldi, Alessandro; Chioda, G.; Ferrari, Maria L.; Finazzi, Guido; Finazzi, Mariachiara; Belotti, Clara; Boroni, C.; Salmoiraghi, S.; Amaru, A.; Golay, Josée; Bortoluzzi, S.; Bisognin, Andrea; Coppe, Alessandro; Saccoman, C.; Manfredini, Rossella; Artuso, Lucia; Bernardis, Isabella; Bianchi, Elisa; Montanari, Monica; Pennucci, Valentina; Prudente, Zelia; Rontauroli, Sebastiano; Rossi, C.; Ruberti, Samantha; Salati, Simona; Tagliafico, Enrico; Tenedini, Elena; Zini, R.

In: PLoS One, Vol. 11, No. 6, e0156990, 01.06.2016.

Research output: Contribution to journalArticle

Campanelli, R, Fois, G, Catarsi, P, Poletto, V, Villani, L, Erba, BG, Maddaluno, L, Jemos, B, Salmoiraghi, S, Guglielmelli, P, Abbonante, V, Di Buduo, CA, Balduini, A, Iurlo, A, Barosi, G, Rosti, V, Massa, M, Vannucchi, AM, Balliu, M, Bartalucci, N, Bogani, C, Bosi, A, Calabresi, L, Corbizzi Fattori, G, Fanelli, T, Fjerza, R, Gesullo, F, Guglielmelli, P, Mannarelli, C, Merli, L, Pacilli, A, Pancrazzi, A, Paoli, C, Pieri, L, Rotunno, G, Sant'Antonio, E, Barosi, G, Abbonante, V, Balduini, A, Bonetti, E, Campanelli, R, Catarsi, P, Di Buduo, CA, Fois, G, Massa, M, Poletto, V, Rosti, V, Villani, L, Cazzola, M, Ambaglio, I, Bernasconi, P, Casetti, CI, Catricala, S, Elena, C, Fugazza, E, Gallì, A, Malcovati, L, Milanesi, C, Pascutto, C, Pietra, D, Ripamonti, F, Rossi, M, Rumi, E, Dejana, E, Breviario, F, Corada, M, Malinverno, M, Rambaldi, A, Chioda, G, Ferrari, ML, Finazzi, G, Finazzi, M, Belotti, C, Boroni, C, Salmoiraghi, S, Amaru, A, Golay, J, Bortoluzzi, S, Bisognin, A, Coppe, A, Saccoman, C, Manfredini, R, Artuso, L, Bernardis, I, Bianchi, E, Montanari, M, Pennucci, V, Prudente, Z, Rontauroli, S, Rossi, C, Ruberti, S, Salati, S, Tagliafico, E, Tenedini, E & Zini, R 2016, 'Tie2 expressing monocytes in the spleen of patients with primary myelofibrosis', PLoS One, vol. 11, no. 6, e0156990. https://doi.org/10.1371/journal.pone.0156990
Campanelli, Rita ; Fois, Gabriela ; Catarsi, Paolo ; Poletto, Valentina ; Villani, Laura ; Erba, Benedetta Gaia ; Maddaluno, Luigi ; Jemos, Basilio ; Salmoiraghi, Silvia ; Guglielmelli, Paola ; Abbonante, Vittorio ; Di Buduo, Christian Andrea ; Balduini, A. ; Iurlo, Alessandra ; Barosi, Giovanni ; Rosti, Vittorio ; Massa, Margherita ; Vannucchi, Alessandro M. ; Balliu, M. ; Bartalucci, Niccolò ; Bogani, Costanza ; Bosi, Alberto ; Calabresi, L. ; Corbizzi Fattori, G. ; Fanelli, Tiziana ; Fjerza, Rajmonda ; Gesullo, F. ; Guglielmelli, Paola ; Mannarelli, C. ; Merli, L. ; Pacilli, Annalisa ; Pancrazzi, A. ; Paoli, Chiara ; Pieri, Lisa ; Rotunno, G. ; Sant'Antonio, Emanuela ; Barosi, G. ; Abbonante, Vittorio ; Balduini, A. ; Bonetti, Elisa ; Campanelli, R. ; Catarsi, P. ; Di Buduo, Christian A. ; Fois, G. ; Massa, M. ; Poletto, V. ; Rosti, V. ; Villani, L. ; Cazzola, Mario ; Ambaglio, Ilaria ; Bernasconi, Paolo ; Casetti, C. I. ; Catricala, Silvia ; Elena, Chiara ; Fugazza, Elena ; Gallì, Anna ; Malcovati, Luca ; Milanesi, Chiara ; Pascutto, Cristiana ; Pietra, Daniela ; Ripamonti, Francesco ; Rossi, Marianna ; Rumi, Elisa ; Dejana, Elisabetta ; Breviario, Ferruccio ; Corada, Monica ; Malinverno, M. ; Rambaldi, Alessandro ; Chioda, G. ; Ferrari, Maria L. ; Finazzi, Guido ; Finazzi, Mariachiara ; Belotti, Clara ; Boroni, C. ; Salmoiraghi, S. ; Amaru, A. ; Golay, Josée ; Bortoluzzi, S. ; Bisognin, Andrea ; Coppe, Alessandro ; Saccoman, C. ; Manfredini, Rossella ; Artuso, Lucia ; Bernardis, Isabella ; Bianchi, Elisa ; Montanari, Monica ; Pennucci, Valentina ; Prudente, Zelia ; Rontauroli, Sebastiano ; Rossi, C. ; Ruberti, Samantha ; Salati, Simona ; Tagliafico, Enrico ; Tenedini, Elena ; Zini, R. / Tie2 expressing monocytes in the spleen of patients with primary myelofibrosis. In: PLoS One. 2016 ; Vol. 11, No. 6.
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title = "Tie2 expressing monocytes in the spleen of patients with primary myelofibrosis",
abstract = "Primary myelofibrosis (PMF) is a Philadelphia-negative (Ph-) myeloproliferative disorder, showing abnormal CD34+ progenitor cell trafficking, splenomegaly, marrow fibrosis leading to extensive extramedullary haematopoiesis, and abnormal neoangiogenesis in either the bone marrow or the spleen. Monocytes expressing the angiopoietin-2 receptor (Tie2) have been shown to support abnormal angiogenic processes in solid tumors through a paracrine action that takes place in proximity to the vessels. In this study we investigated the frequency of Tie2 expressing monocytes in the spleen tissue samples of patients with PMF, and healthy subjects (CTRLs), and evaluated their possible role in favouring spleen angiogenesis. We show by confocal microscopy that in the spleen tissue of patients with PMF, but not of CTRLs, the most of the CD14+ cells are Tie2+ and are close to vessels; by flow cytometry, we found that Tie2 expressing monocytes were Tie2+CD14lowCD16brightCDL62-CCR2- (TEMs) and their frequency was higher (p = 0.008) in spleen tissue-derived mononuclear cells (MNCs) of patients with PMF than in spleen tissue-derived MNCs from CTRLs undergoing splenectomy for abdominal trauma. By in vitro angiogenesis assay we evidenced that conditioned medium of immunomagnetically selected spleen tissue derived CD14+ cells of patients with PMF induced a denser tube like net than that of CTRLs; in addition, CD14+Tie2+ cells sorted from spleen tissue derived single cell suspension of patients with PMF show a higher expression of genes involved in angiogenesis than that found in CTRLs. Our results document the enrichment of Tie2+ monocytes expressing angiogenic genes in the spleen of patients with PMF, suggesting a role for these cells in starting/maintaining the pathological angiogenesis in this organ.",
author = "Rita Campanelli and Gabriela Fois and Paolo Catarsi and Valentina Poletto and Laura Villani and Erba, {Benedetta Gaia} and Luigi Maddaluno and Basilio Jemos and Silvia Salmoiraghi and Paola Guglielmelli and Vittorio Abbonante and {Di Buduo}, {Christian Andrea} and A. Balduini and Alessandra Iurlo and Giovanni Barosi and Vittorio Rosti and Margherita Massa and Vannucchi, {Alessandro M.} and M. Balliu and Niccol{\`o} Bartalucci and Costanza Bogani and Alberto Bosi and L. Calabresi and {Corbizzi Fattori}, G. and Tiziana Fanelli and Rajmonda Fjerza and F. Gesullo and Paola Guglielmelli and C. Mannarelli and L. Merli and Annalisa Pacilli and A. Pancrazzi and Chiara Paoli and Lisa Pieri and G. Rotunno and Emanuela Sant'Antonio and G. Barosi and Vittorio Abbonante and A. Balduini and Elisa Bonetti and R. Campanelli and P. Catarsi and {Di Buduo}, {Christian A.} and G. Fois and M. Massa and V. Poletto and V. Rosti and L. Villani and Mario Cazzola and Ilaria Ambaglio and Paolo Bernasconi and Casetti, {C. I.} and Silvia Catricala and Chiara Elena and Elena Fugazza and Anna Gall{\`i} and Luca Malcovati and Chiara Milanesi and Cristiana Pascutto and Daniela Pietra and Francesco Ripamonti and Marianna Rossi and Elisa Rumi and Elisabetta Dejana and Ferruccio Breviario and Monica Corada and M. Malinverno and Alessandro Rambaldi and G. Chioda and Ferrari, {Maria L.} and Guido Finazzi and Mariachiara Finazzi and Clara Belotti and C. Boroni and S. Salmoiraghi and A. Amaru and Jos{\'e}e Golay and S. Bortoluzzi and Andrea Bisognin and Alessandro Coppe and C. Saccoman and Rossella Manfredini and Lucia Artuso and Isabella Bernardis and Elisa Bianchi and Monica Montanari and Valentina Pennucci and Zelia Prudente and Sebastiano Rontauroli and C. Rossi and Samantha Ruberti and Simona Salati and Enrico Tagliafico and Elena Tenedini and R. Zini",
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T1 - Tie2 expressing monocytes in the spleen of patients with primary myelofibrosis

AU - Campanelli, Rita

AU - Fois, Gabriela

AU - Catarsi, Paolo

AU - Poletto, Valentina

AU - Villani, Laura

AU - Erba, Benedetta Gaia

AU - Maddaluno, Luigi

AU - Jemos, Basilio

AU - Salmoiraghi, Silvia

AU - Guglielmelli, Paola

AU - Abbonante, Vittorio

AU - Di Buduo, Christian Andrea

AU - Balduini, A.

AU - Iurlo, Alessandra

AU - Barosi, Giovanni

AU - Rosti, Vittorio

AU - Massa, Margherita

AU - Vannucchi, Alessandro M.

AU - Balliu, M.

AU - Bartalucci, Niccolò

AU - Bogani, Costanza

AU - Bosi, Alberto

AU - Calabresi, L.

AU - Corbizzi Fattori, G.

AU - Fanelli, Tiziana

AU - Fjerza, Rajmonda

AU - Gesullo, F.

AU - Guglielmelli, Paola

AU - Mannarelli, C.

AU - Merli, L.

AU - Pacilli, Annalisa

AU - Pancrazzi, A.

AU - Paoli, Chiara

AU - Pieri, Lisa

AU - Rotunno, G.

AU - Sant'Antonio, Emanuela

AU - Barosi, G.

AU - Abbonante, Vittorio

AU - Balduini, A.

AU - Bonetti, Elisa

AU - Campanelli, R.

AU - Catarsi, P.

AU - Di Buduo, Christian A.

AU - Fois, G.

AU - Massa, M.

AU - Poletto, V.

AU - Rosti, V.

AU - Villani, L.

AU - Cazzola, Mario

AU - Ambaglio, Ilaria

AU - Bernasconi, Paolo

AU - Casetti, C. I.

AU - Catricala, Silvia

AU - Elena, Chiara

AU - Fugazza, Elena

AU - Gallì, Anna

AU - Malcovati, Luca

AU - Milanesi, Chiara

AU - Pascutto, Cristiana

AU - Pietra, Daniela

AU - Ripamonti, Francesco

AU - Rossi, Marianna

AU - Rumi, Elisa

AU - Dejana, Elisabetta

AU - Breviario, Ferruccio

AU - Corada, Monica

AU - Malinverno, M.

AU - Rambaldi, Alessandro

AU - Chioda, G.

AU - Ferrari, Maria L.

AU - Finazzi, Guido

AU - Finazzi, Mariachiara

AU - Belotti, Clara

AU - Boroni, C.

AU - Salmoiraghi, S.

AU - Amaru, A.

AU - Golay, Josée

AU - Bortoluzzi, S.

AU - Bisognin, Andrea

AU - Coppe, Alessandro

AU - Saccoman, C.

AU - Manfredini, Rossella

AU - Artuso, Lucia

AU - Bernardis, Isabella

AU - Bianchi, Elisa

AU - Montanari, Monica

AU - Pennucci, Valentina

AU - Prudente, Zelia

AU - Rontauroli, Sebastiano

AU - Rossi, C.

AU - Ruberti, Samantha

AU - Salati, Simona

AU - Tagliafico, Enrico

AU - Tenedini, Elena

AU - Zini, R.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Primary myelofibrosis (PMF) is a Philadelphia-negative (Ph-) myeloproliferative disorder, showing abnormal CD34+ progenitor cell trafficking, splenomegaly, marrow fibrosis leading to extensive extramedullary haematopoiesis, and abnormal neoangiogenesis in either the bone marrow or the spleen. Monocytes expressing the angiopoietin-2 receptor (Tie2) have been shown to support abnormal angiogenic processes in solid tumors through a paracrine action that takes place in proximity to the vessels. In this study we investigated the frequency of Tie2 expressing monocytes in the spleen tissue samples of patients with PMF, and healthy subjects (CTRLs), and evaluated their possible role in favouring spleen angiogenesis. We show by confocal microscopy that in the spleen tissue of patients with PMF, but not of CTRLs, the most of the CD14+ cells are Tie2+ and are close to vessels; by flow cytometry, we found that Tie2 expressing monocytes were Tie2+CD14lowCD16brightCDL62-CCR2- (TEMs) and their frequency was higher (p = 0.008) in spleen tissue-derived mononuclear cells (MNCs) of patients with PMF than in spleen tissue-derived MNCs from CTRLs undergoing splenectomy for abdominal trauma. By in vitro angiogenesis assay we evidenced that conditioned medium of immunomagnetically selected spleen tissue derived CD14+ cells of patients with PMF induced a denser tube like net than that of CTRLs; in addition, CD14+Tie2+ cells sorted from spleen tissue derived single cell suspension of patients with PMF show a higher expression of genes involved in angiogenesis than that found in CTRLs. Our results document the enrichment of Tie2+ monocytes expressing angiogenic genes in the spleen of patients with PMF, suggesting a role for these cells in starting/maintaining the pathological angiogenesis in this organ.

AB - Primary myelofibrosis (PMF) is a Philadelphia-negative (Ph-) myeloproliferative disorder, showing abnormal CD34+ progenitor cell trafficking, splenomegaly, marrow fibrosis leading to extensive extramedullary haematopoiesis, and abnormal neoangiogenesis in either the bone marrow or the spleen. Monocytes expressing the angiopoietin-2 receptor (Tie2) have been shown to support abnormal angiogenic processes in solid tumors through a paracrine action that takes place in proximity to the vessels. In this study we investigated the frequency of Tie2 expressing monocytes in the spleen tissue samples of patients with PMF, and healthy subjects (CTRLs), and evaluated their possible role in favouring spleen angiogenesis. We show by confocal microscopy that in the spleen tissue of patients with PMF, but not of CTRLs, the most of the CD14+ cells are Tie2+ and are close to vessels; by flow cytometry, we found that Tie2 expressing monocytes were Tie2+CD14lowCD16brightCDL62-CCR2- (TEMs) and their frequency was higher (p = 0.008) in spleen tissue-derived mononuclear cells (MNCs) of patients with PMF than in spleen tissue-derived MNCs from CTRLs undergoing splenectomy for abdominal trauma. By in vitro angiogenesis assay we evidenced that conditioned medium of immunomagnetically selected spleen tissue derived CD14+ cells of patients with PMF induced a denser tube like net than that of CTRLs; in addition, CD14+Tie2+ cells sorted from spleen tissue derived single cell suspension of patients with PMF show a higher expression of genes involved in angiogenesis than that found in CTRLs. Our results document the enrichment of Tie2+ monocytes expressing angiogenic genes in the spleen of patients with PMF, suggesting a role for these cells in starting/maintaining the pathological angiogenesis in this organ.

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