Tightly-bound non-histone proteins in different nucleosome-like subpopulations from pig kidney chromatin.

P. Caiafa, A. Tomassetti, S. Mastrantonio, A. Reale, M. Spinelli, R. Strom

Research output: Contribution to journalArticlepeer-review

Abstract

By differential sucrose gradient centrifugation of pig kidney chromatin in the presence or absence of Na-EDTA and under varying ionic strength conditions, three nucleosome-like subpopulations with different buoyant densities can be obtained. These particles, on the basis of their histones and HMG protein pattern, of the 5-methylcytosine level of their DNA and of the RNA polymerase activity associated with them, can be considered as originating from chromatin fractions differently involved in gene expression. Two-dimensional electrophoresis of the tightly-bound non-histone proteins shows a distinct pattern for each subpopulation, such protein components being notably present in restricted numbers but in high amounts in the subpopulation which was apparently derived from condensed heterochromatin.

Original languageEnglish
Pages (from-to)39-45
Number of pages7
JournalCell Biochemistry and Function
Volume6
Issue number1
Publication statusPublished - Jan 1988

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry

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