Abstract
By differential sucrose gradient centrifugation of pig kidney chromatin in the presence or absence of Na-EDTA and under varying ionic strength conditions, three nucleosome-like subpopulations with different buoyant densities can be obtained. These particles, on the basis of their histones and HMG protein pattern, of the 5-methylcytosine level of their DNA and of the RNA polymerase activity associated with them, can be considered as originating from chromatin fractions differently involved in gene expression. Two-dimensional electrophoresis of the tightly-bound non-histone proteins shows a distinct pattern for each subpopulation, such protein components being notably present in restricted numbers but in high amounts in the subpopulation which was apparently derived from condensed heterochromatin.
Original language | English |
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Pages (from-to) | 39-45 |
Number of pages | 7 |
Journal | Cell Biochemistry and Function |
Volume | 6 |
Issue number | 1 |
Publication status | Published - Jan 1988 |
ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry