Time course of nigrostriatal system changes after tract hemisection and effects of ganglioside treatment: a multiple retrograde fluorescent tracing study

Marco Molinari, Adriana Consolazione, Alberta Leon, Marina Bentivoglio

Research output: Contribution to journalArticlepeer-review

Abstract

The postlesion phenomena of nigrostriatal neurons following partial tract hemisection have been studied with retrograde axonal transport of dyes in rats treated with saline or GM1 ganglioside (30 mg/kg, i.p., daily for varying times). The time course of the plastic changes in both experimental animal groups was determined by a double retrograde fluorescent tracing technique, utilizing Fast blue (FB) as prelesion tracer, and Diamidino yellow (DY) as postlesion tracer. The data show that the number of nigral cells retrogradely labeled by the prelesion intrastriatal injection of FB declined progressively to 50% of their initial number over 3 weeks. The ganglioside treatment did not significantly influence this temporal pattern of labeled cell loss. By DY-labeling, an almost complete block of the retrograde axonal transport was observed up to 1 week postlesion and only after 3 weeks a partial recovery was evident, positively influenced by the GM1 treatment. Therefore, together with the findings on nigrostriatal cell loss, these data indicate that ganglioside treatment favors and/or accelerates the recovery of axonal transport of surviving nigral neurons but does not significantly affect their degenerative rate in the first 3 weeks after the lesion.

Original languageEnglish
Pages (from-to)126-133
Number of pages8
JournalBrain Research
Volume570
Issue number1-2
DOIs
Publication statusPublished - Jan 20 1992

Keywords

  • Axonal transport
  • Fluorescent tracing
  • Ganglioside
  • Neuroplasticity
  • Nigrostriatal tract

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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