Time-course of spontaneous transformation of CD-1 mouse embryonic fibroblasts

Ottavio Rossi, Ottavia Barbieri, Guido Frosina

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The spontaneous transformation of cultured mouse embryonic fibroblasts (MEF) from the outbred CD-1 mouse strain was investigated. Four MEF clones, obtained from four different mouse embryos, were cultured continuously for periods ranging from 300 to 400 days. MEF cells entered a "crisis" with acute loss of proliferation capacity after 16 ± 2 days of culture. Surviving cells gradually acquired properties of transformed cells with the following times: the capacity to grow at a cell density of 105 cells/cm2 was acquired after 269 ± 45 days. Twenty-five % anchorage-dependent colony forming ability was observed after 255 ± 39 days. 0.5% anchorage-independent colony forming ability was observed after 290 ± 54 days. Protective factors capable of significantly delaying the above events might be investigated with the MEF system.

Original languageEnglish
Pages (from-to)1373-1377
Number of pages5
JournalAnticancer Research
Volume23
Issue number2 B
Publication statusPublished - Mar 2003

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Fibroblasts
Embryonic Structures
Clone Cells
Cell Count

Keywords

  • DNA repair
  • Enbryonic fibroblasts
  • Immortalization
  • Oxidative damage

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Time-course of spontaneous transformation of CD-1 mouse embryonic fibroblasts. / Rossi, Ottavio; Barbieri, Ottavia; Frosina, Guido.

In: Anticancer Research, Vol. 23, No. 2 B, 03.2003, p. 1373-1377.

Research output: Contribution to journalArticle

Rossi, Ottavio ; Barbieri, Ottavia ; Frosina, Guido. / Time-course of spontaneous transformation of CD-1 mouse embryonic fibroblasts. In: Anticancer Research. 2003 ; Vol. 23, No. 2 B. pp. 1373-1377.
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