Time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites

M. D'incalci, E. Erba, G. Balconi, L. Morasca, S. Garattini

Research output: Contribution to journalArticle

Abstract

The cytotoxicity of hexamethylmelamine (HMM) and its metabolites pentamethylmelamine (PMM), N,2,2,4,6-tetramethylmelamine (TMM) and hydroxymethylpentamethylmelamine (HMPMM) and of the alkylating agent triethylene-melamine (TEM) were studied on a cell line derived from a human ovarian cancer, by measuring [3H]TdR uptake. After 24 h of incubation all the tested compounds inhibited [3H]TdR uptake, but only at a concentration of 100μg/ml. However, after 120 h incubation concentrations of 0.1-10 μg/ml resulted in highly significant cytotoxicity. HMPMM and TEM were the most active and their effect was not reversed 72 h after their removal. In our in vitro system no metabolism of HMM was observed.

Original languageEnglish
Pages (from-to)630-635
Number of pages6
JournalBritish Journal of Cancer
Volume41
Issue number4
DOIs
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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