Abstract
The cytotoxicity of hexamethylmelamine (HMM) and its metabolites pentamethylmelamine (PMM), N,2,2,4,6-tetramethylmelamine (TMM) and hydroxymethylpentamethylmelamine (HMPMM) and of the alkylating agent triethylene-melamine (TEM) were studied on a cell line derived from a human ovarian cancer, by measuring [3H]TdR uptake. After 24 h of incubation all the tested compounds inhibited [3H]TdR uptake, but only at a concentration of 100μg/ml. However, after 120 h incubation concentrations of 0.1-10 μg/ml resulted in highly significant cytotoxicity. HMPMM and TEM were the most active and their effect was not reversed 72 h after their removal. In our in vitro system no metabolism of HMM was observed.
| Original language | English |
|---|---|
| Pages (from-to) | 630-635 |
| Number of pages | 6 |
| Journal | British Journal of Cancer |
| Volume | 41 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1980 |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
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Time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites. / D'incalci, M.; Erba, E.; Balconi, G.; Morasca, L.; Garattini, S.
In: British Journal of Cancer, Vol. 41, No. 4, 1980, p. 630-635.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Time dependence of the in vitro cytotoxicity of hexamethylmelamine and its metabolites
AU - D'incalci, M.
AU - Erba, E.
AU - Balconi, G.
AU - Morasca, L.
AU - Garattini, S.
PY - 1980
Y1 - 1980
N2 - The cytotoxicity of hexamethylmelamine (HMM) and its metabolites pentamethylmelamine (PMM), N,2,2,4,6-tetramethylmelamine (TMM) and hydroxymethylpentamethylmelamine (HMPMM) and of the alkylating agent triethylene-melamine (TEM) were studied on a cell line derived from a human ovarian cancer, by measuring [3H]TdR uptake. After 24 h of incubation all the tested compounds inhibited [3H]TdR uptake, but only at a concentration of 100μg/ml. However, after 120 h incubation concentrations of 0.1-10 μg/ml resulted in highly significant cytotoxicity. HMPMM and TEM were the most active and their effect was not reversed 72 h after their removal. In our in vitro system no metabolism of HMM was observed.
AB - The cytotoxicity of hexamethylmelamine (HMM) and its metabolites pentamethylmelamine (PMM), N,2,2,4,6-tetramethylmelamine (TMM) and hydroxymethylpentamethylmelamine (HMPMM) and of the alkylating agent triethylene-melamine (TEM) were studied on a cell line derived from a human ovarian cancer, by measuring [3H]TdR uptake. After 24 h of incubation all the tested compounds inhibited [3H]TdR uptake, but only at a concentration of 100μg/ml. However, after 120 h incubation concentrations of 0.1-10 μg/ml resulted in highly significant cytotoxicity. HMPMM and TEM were the most active and their effect was not reversed 72 h after their removal. In our in vitro system no metabolism of HMM was observed.
UR - http://www.scopus.com/inward/record.url?scp=0018836777&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018836777&partnerID=8YFLogxK
U2 - 10.1038/bjc.1980.106
DO - 10.1038/bjc.1980.106
M3 - Article
C2 - 6770884
AN - SCOPUS:0018836777
VL - 41
SP - 630
EP - 635
JO - British Journal of Cancer
JF - British Journal of Cancer
SN - 0007-0920
IS - 4
ER -