Time-lapse dynamics of the mouse oocyte chromatin organisation during meiotic resumption

Martina Belli, Giulia Vigone, Valeria Merico, Carlo Alberto Redi, Silvia Garagna, Maurizio Zuccotti

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In the mammalian oocyte, distinct patterns of centromeres and pericentromeric heterochromatin localisation correlate with the gamete's developmental competence. Mouse antral oocytes display two main types of chromatin organisation: SN oocytes, with a ring of Hoechst-positive chromatin surrounding the nucleolus, and NSN oocytes lacking this ring. When matured to MII and fertilised, only SN oocytes develop beyond the 2-cell, and reach full term. To give detailed information on the dynamics of the SN or NSN chromatin during meiosis resumption, we performed a 9 hr time-lapse observation. The main significant differences recorded are: (1) reduction of the nuclear area only in SN oocytes; (2) 17 min delay of GVBD in NSN oocytes; (3) chromatin condensation, after GVBD, in SN oocytes; (4) formation of 4-5 CHCs in SN oocytes; (5) increase of the perivitelline space, 57 min later in NSN oocytes; (6) formation of a rosette-like disposition of CHCs, 84 min later in SN oocytes; (7) appearance of the MI plate 40 min later in NSN oocytes. Overall, we described a pathway of transition from the GV to the MII stage that is punctuated of discrete recordable events showing their specificity and occurring with different time kinetics in the two types of oocytes.

Original languageEnglish
Article number207357
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014

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Chromatin
Oocytes
Condensation
Display devices
Kinetics
Rosette Formation
Heterochromatin
Centromere
Meiosis
Germ Cells
Mental Competency
Observation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Time-lapse dynamics of the mouse oocyte chromatin organisation during meiotic resumption. / Belli, Martina; Vigone, Giulia; Merico, Valeria; Redi, Carlo Alberto; Garagna, Silvia; Zuccotti, Maurizio.

In: BioMed Research International, Vol. 2014, 207357, 2014.

Research output: Contribution to journalArticle

Belli, Martina ; Vigone, Giulia ; Merico, Valeria ; Redi, Carlo Alberto ; Garagna, Silvia ; Zuccotti, Maurizio. / Time-lapse dynamics of the mouse oocyte chromatin organisation during meiotic resumption. In: BioMed Research International. 2014 ; Vol. 2014.
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