Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity

G. Valentini, C. D'Andrea, D. Comelli, A. Pifferi, P. Taroni, A. Torricelli, R. Cubeddu, C. Battaglia, C. Consolandi, G. Salani, L. Rossi-Bernardi, G. De Bellis

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We describe a novel technique for DNA-microarray reading based on time-resolved fluorescence measurements. We used an intensified CCD camera with picosecond resolution to acquire a set of time-delayed fluorescence images from a mutation DNA microarray marked with cyanine 3. We measured the fluorescence lifetimes of the marker and the background separately, and we used this information to calculate the amplitude map of the marker, starting from the time-delayed images. This procedure allowed us to identify hybridized spots that are not visible in fluorescence images acquired with continuous-wave detection.

Original languageEnglish
Pages (from-to)1648-1650
Number of pages3
JournalOptics Letters
Volume25
Issue number22
Publication statusPublished - Nov 15 2000

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charge coupled devices
deoxyribonucleic acid
fluorescence
sensitivity
markers
mutations
CCD cameras
continuous radiation
life (durability)

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Valentini, G., D'Andrea, C., Comelli, D., Pifferi, A., Taroni, P., Torricelli, A., ... De Bellis, G. (2000). Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity. Optics Letters, 25(22), 1648-1650.

Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity. / Valentini, G.; D'Andrea, C.; Comelli, D.; Pifferi, A.; Taroni, P.; Torricelli, A.; Cubeddu, R.; Battaglia, C.; Consolandi, C.; Salani, G.; Rossi-Bernardi, L.; De Bellis, G.

In: Optics Letters, Vol. 25, No. 22, 15.11.2000, p. 1648-1650.

Research output: Contribution to journalArticle

Valentini, G, D'Andrea, C, Comelli, D, Pifferi, A, Taroni, P, Torricelli, A, Cubeddu, R, Battaglia, C, Consolandi, C, Salani, G, Rossi-Bernardi, L & De Bellis, G 2000, 'Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity', Optics Letters, vol. 25, no. 22, pp. 1648-1650.
Valentini G, D'Andrea C, Comelli D, Pifferi A, Taroni P, Torricelli A et al. Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity. Optics Letters. 2000 Nov 15;25(22):1648-1650.
Valentini, G. ; D'Andrea, C. ; Comelli, D. ; Pifferi, A. ; Taroni, P. ; Torricelli, A. ; Cubeddu, R. ; Battaglia, C. ; Consolandi, C. ; Salani, G. ; Rossi-Bernardi, L. ; De Bellis, G. / Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity. In: Optics Letters. 2000 ; Vol. 25, No. 22. pp. 1648-1650.
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