Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity

G. Valentini; C. D'Andrea; D. Comelli; A. Pifferi; P. Taroni; A. Torricelli; R. Cubeddu; C. Battaglia; C. Consolandi; G. Salani; L. Rossi-Bernardi; G. De Bellis

Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity

G. Valentini, C. D'Andrea, D. Comelli, A. Pifferi, P. Taroni, A. Torricelli, R. Cubeddu, C. Battaglia, C. Consolandi, G. Salani, L. Rossi-Bernardi, G. De Bellis

IRCCS Multimedica

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a novel technique for DNA-microarray reading based on time-resolved fluorescence measurements. We used an intensified CCD camera with picosecond resolution to acquire a set of time-delayed fluorescence images from a mutation DNA microarray marked with cyanine 3. We measured the fluorescence lifetimes of the marker and the background separately, and we used this information to calculate the amplitude map of the marker, starting from the time-delayed images. This procedure allowed us to identify hybridized spots that are not visible in fluorescence images acquired with continuous-wave detection.

Original language	English
Pages (from-to)	1648-1650
Number of pages	3
Journal	Optics Letters
Volume	25
Issue number	22
Publication status	Published - Nov 15 2000

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

Cite this

Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity. / Valentini, G.; D'Andrea, C.; Comelli, D.; Pifferi, A.; Taroni, P.; Torricelli, A.; Cubeddu, R.; Battaglia, C.; Consolandi, C.; Salani, G.; Rossi-Bernardi, L.; De Bellis, G.

In: Optics Letters, Vol. 25, No. 22, 15.11.2000, p. 1648-1650.

Research output: Contribution to journal › Article › peer-review

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abstract = "We describe a novel technique for DNA-microarray reading based on time-resolved fluorescence measurements. We used an intensified CCD camera with picosecond resolution to acquire a set of time-delayed fluorescence images from a mutation DNA microarray marked with cyanine 3. We measured the fluorescence lifetimes of the marker and the background separately, and we used this information to calculate the amplitude map of the marker, starting from the time-delayed images. This procedure allowed us to identify hybridized spots that are not visible in fluorescence images acquired with continuous-wave detection.",

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AU - Valentini, G.

AU - D'Andrea, C.

AU - Comelli, D.

AU - Pifferi, A.

AU - Taroni, P.

AU - Torricelli, A.

AU - Cubeddu, R.

AU - Battaglia, C.

AU - Consolandi, C.

AU - Salani, G.

AU - Rossi-Bernardi, L.

AU - De Bellis, G.

PY - 2000/11/15

Y1 - 2000/11/15

N2 - We describe a novel technique for DNA-microarray reading based on time-resolved fluorescence measurements. We used an intensified CCD camera with picosecond resolution to acquire a set of time-delayed fluorescence images from a mutation DNA microarray marked with cyanine 3. We measured the fluorescence lifetimes of the marker and the background separately, and we used this information to calculate the amplitude map of the marker, starting from the time-delayed images. This procedure allowed us to identify hybridized spots that are not visible in fluorescence images acquired with continuous-wave detection.

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Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity

Abstract

ASJC Scopus subject areas

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