Abstract
We describe a novel technique for DNA-microarray reading based on time-resolved fluorescence measurements. We used an intensified CCD camera with picosecond resolution to acquire a set of time-delayed fluorescence images from a mutation DNA microarray marked with cyanine 3. We measured the fluorescence lifetimes of the marker and the background separately, and we used this information to calculate the amplitude map of the marker, starting from the time-delayed images. This procedure allowed us to identify hybridized spots that are not visible in fluorescence images acquired with continuous-wave detection.
| Original language | English |
|---|---|
| Pages (from-to) | 1648-1650 |
| Number of pages | 3 |
| Journal | Optics Letters |
| Volume | 25 |
| Issue number | 22 |
| Publication status | Published - Nov 15 2000 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity. / Valentini, G.; D'Andrea, C.; Comelli, D.; Pifferi, A.; Taroni, P.; Torricelli, A.; Cubeddu, R.; Battaglia, C.; Consolandi, C.; Salani, G.; Rossi-Bernardi, L.; De Bellis, G.
In: Optics Letters, Vol. 25, No. 22, 15.11.2000, p. 1648-1650.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity
AU - Valentini, G.
AU - D'Andrea, C.
AU - Comelli, D.
AU - Pifferi, A.
AU - Taroni, P.
AU - Torricelli, A.
AU - Cubeddu, R.
AU - Battaglia, C.
AU - Consolandi, C.
AU - Salani, G.
AU - Rossi-Bernardi, L.
AU - De Bellis, G.
PY - 2000/11/15
Y1 - 2000/11/15
N2 - We describe a novel technique for DNA-microarray reading based on time-resolved fluorescence measurements. We used an intensified CCD camera with picosecond resolution to acquire a set of time-delayed fluorescence images from a mutation DNA microarray marked with cyanine 3. We measured the fluorescence lifetimes of the marker and the background separately, and we used this information to calculate the amplitude map of the marker, starting from the time-delayed images. This procedure allowed us to identify hybridized spots that are not visible in fluorescence images acquired with continuous-wave detection.
AB - We describe a novel technique for DNA-microarray reading based on time-resolved fluorescence measurements. We used an intensified CCD camera with picosecond resolution to acquire a set of time-delayed fluorescence images from a mutation DNA microarray marked with cyanine 3. We measured the fluorescence lifetimes of the marker and the background separately, and we used this information to calculate the amplitude map of the marker, starting from the time-delayed images. This procedure allowed us to identify hybridized spots that are not visible in fluorescence images acquired with continuous-wave detection.
UR - http://www.scopus.com/inward/record.url?scp=0001010563&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0001010563&partnerID=8YFLogxK
M3 - Article
C2 - 18066303
AN - SCOPUS:0001010563
VL - 25
SP - 1648
EP - 1650
JO - Optics Letters
JF - Optics Letters
SN - 0146-9592
IS - 22
ER -