Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity

G. Valentini, C. D'Andrea, D. Comelli, A. Pifferi, P. Taroni, A. Torricelli, R. Cubeddu, C. Battaglia, C. Consolandi, G. Salani, L. Rossi-Bernardi, G. De Bellis

Research output: Contribution to journalArticlepeer-review

Abstract

We describe a novel technique for DNA-microarray reading based on time-resolved fluorescence measurements. We used an intensified CCD camera with picosecond resolution to acquire a set of time-delayed fluorescence images from a mutation DNA microarray marked with cyanine 3. We measured the fluorescence lifetimes of the marker and the background separately, and we used this information to calculate the amplitude map of the marker, starting from the time-delayed images. This procedure allowed us to identify hybridized spots that are not visible in fluorescence images acquired with continuous-wave detection.

Original languageEnglish
Pages (from-to)1648-1650
Number of pages3
JournalOptics Letters
Volume25
Issue number22
Publication statusPublished - Nov 15 2000

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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