Abstract
A method is reported to set up a standard competitive TR-FIA. A simple and inexpensive way to prepare reagents and carry out operations is presented as well, with the aim to make it possible to perform a very sensitive analytical procedure in a personalized way within a nondedicated biochemistry laboratory. This protocol is general and can be easily modified with consideration to the analytical target. Once the antibody is available, both its labeling with diethylenetriaminepentaacetic acid dianhydride and Eu3+, and the setting-up of the assay with measurement of europium ion time- resolved fluorescence in a home-made enhancement solution become feasible.
| Original language | English |
|---|---|
| Pages (from-to) | 215-222 |
| Number of pages | 8 |
| Journal | Molecular Biotechnology |
| Volume | 13 |
| Issue number | 3 |
| Publication status | Published - Dec 15 1999 |
Keywords
- Europium ion
- Flourescence enhancement
- Fluoimmunoassay
- Time-resolution
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology