Time-resolved fluoroimmunoassay

Paolo Degan, Adriano Podestà, Giorgio Montagnoli

Research output: Contribution to journalArticlepeer-review

Abstract

A method is reported to set up a standard competitive TR-FIA. A simple and inexpensive way to prepare reagents and carry out operations is presented as well, with the aim to make it possible to perform a very sensitive analytical procedure in a personalized way within a nondedicated biochemistry laboratory. This protocol is general and can be easily modified with consideration to the analytical target. Once the antibody is available, both its labeling with diethylenetriaminepentaacetic acid dianhydride and Eu3+, and the setting-up of the assay with measurement of europium ion time- resolved fluorescence in a home-made enhancement solution become feasible.

Original languageEnglish
Pages (from-to)215-222
Number of pages8
JournalMolecular Biotechnology
Volume13
Issue number3
Publication statusPublished - Dec 15 1999

Keywords

  • Europium ion
  • Flourescence enhancement
  • Fluoimmunoassay
  • Time-resolution

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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