TY - JOUR
T1 - Time resolved fluoroimmunoassay of 7-methyl-2′-deoxyguanosine imidazole (ring open)
AU - Degan, Paolo
AU - Montesano, Ruggero
AU - Abbondandolo, Angelo
AU - Montagnoli, Giorgio
PY - 1990/10/4
Y1 - 1990/10/4
N2 - A solid-phase competitive time-resolved fluoroimmunoassay for 7-methyl-2′-deoxyguanosine imidazole (ring open) is described, based on highly specific hemocyanin carrier rabbit antibodies, modified with europium chelates. Eu3+ photoluminescence was detected in a novel micellar solution. The assay was validated both by comparing it with an ELISA and by analysing DNA samples, alkylated either 'in vitro' or 'in vivo' by dimethyl sulfate. The proposed assay proved to be sensitive, simple and reliable. It should be of value, together with other immunoassays for methylated DNA bases, in assessing human environmental exposure to alkylating agents such as nitrosamines, thereby providing a powerful tool in epidemiological investigations.
AB - A solid-phase competitive time-resolved fluoroimmunoassay for 7-methyl-2′-deoxyguanosine imidazole (ring open) is described, based on highly specific hemocyanin carrier rabbit antibodies, modified with europium chelates. Eu3+ photoluminescence was detected in a novel micellar solution. The assay was validated both by comparing it with an ELISA and by analysing DNA samples, alkylated either 'in vitro' or 'in vivo' by dimethyl sulfate. The proposed assay proved to be sensitive, simple and reliable. It should be of value, together with other immunoassays for methylated DNA bases, in assessing human environmental exposure to alkylating agents such as nitrosamines, thereby providing a powerful tool in epidemiological investigations.
KW - 7-Methyl-2′-deoxyguanosine
KW - DNA methylation
KW - ELISA
KW - Fluoroimmunoassay
KW - Time-resolved fluorescence
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U2 - 10.1016/0022-1759(90)90325-P
DO - 10.1016/0022-1759(90)90325-P
M3 - Article
C2 - 2212684
AN - SCOPUS:0025115709
VL - 133
SP - 119
EP - 125
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1
ER -