Time resolved fluoroimmunoassay of 7-methyl-2′-deoxyguanosine imidazole (ring open)

Paolo Degan, Ruggero Montesano, Angelo Abbondandolo, Giorgio Montagnoli

Research output: Contribution to journalArticle

Abstract

A solid-phase competitive time-resolved fluoroimmunoassay for 7-methyl-2′-deoxyguanosine imidazole (ring open) is described, based on highly specific hemocyanin carrier rabbit antibodies, modified with europium chelates. Eu3+ photoluminescence was detected in a novel micellar solution. The assay was validated both by comparing it with an ELISA and by analysing DNA samples, alkylated either 'in vitro' or 'in vivo' by dimethyl sulfate. The proposed assay proved to be sensitive, simple and reliable. It should be of value, together with other immunoassays for methylated DNA bases, in assessing human environmental exposure to alkylating agents such as nitrosamines, thereby providing a powerful tool in epidemiological investigations.

Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalJournal of Immunological Methods
Volume133
Issue number1
DOIs
Publication statusPublished - Oct 4 1990

    Fingerprint

Keywords

  • 7-Methyl-2′-deoxyguanosine
  • DNA methylation
  • ELISA
  • Fluoroimmunoassay
  • Time-resolved fluorescence

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this