Time resolved fluoroimmunoassay of 7-methyl-2′-deoxyguanosine imidazole (ring open)

Paolo Degan; Ruggero Montesano; Angelo Abbondandolo; Giorgio Montagnoli

doi:10.1016/0022-1759(90)90325-P

Time resolved fluoroimmunoassay of 7-methyl-2′-deoxyguanosine imidazole (ring open)

Paolo Degan, Ruggero Montesano, Angelo Abbondandolo, Giorgio Montagnoli

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article

Abstract

A solid-phase competitive time-resolved fluoroimmunoassay for 7-methyl-2′-deoxyguanosine imidazole (ring open) is described, based on highly specific hemocyanin carrier rabbit antibodies, modified with europium chelates. Eu³⁺ photoluminescence was detected in a novel micellar solution. The assay was validated both by comparing it with an ELISA and by analysing DNA samples, alkylated either 'in vitro' or 'in vivo' by dimethyl sulfate. The proposed assay proved to be sensitive, simple and reliable. It should be of value, together with other immunoassays for methylated DNA bases, in assessing human environmental exposure to alkylating agents such as nitrosamines, thereby providing a powerful tool in epidemiological investigations.

Original language	English
Pages (from-to)	119-125
Number of pages	7
Journal	Journal of Immunological Methods
Volume	133
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(90)90325-P
Publication status	Published - Oct 4 1990

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Keywords

7-Methyl-2′-deoxyguanosine
DNA methylation
ELISA
Fluoroimmunoassay
Time-resolved fluorescence

ASJC Scopus subject areas

Biotechnology
Immunology

Cite this

Degan, P., Montesano, R., Abbondandolo, A., & Montagnoli, G. (1990). Time resolved fluoroimmunoassay of 7-methyl-2′-deoxyguanosine imidazole (ring open). Journal of Immunological Methods, 133(1), 119-125. https://doi.org/10.1016/0022-1759(90)90325-P

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10.1016/0022-1759(90)90325-P