Timing of mouse Xist expression during preimplantation development

Nota Di Maurizio Zuccotti, Michele Boiani, Silvia Garagna, Carlo Alberto Redi, E. Cappanna

Research output: Contribution to journalArticlepeer-review

Abstract

The X-linked Xist gene is imprinted and is expressed from the paternal allele only in preimplantation development. Mouse Xist was analysed in preimplantation embryos. We found that the onset of Xist expression occurs at the G2-phase of the 2-cell embryos. At this same stage of development a number of newly synthesised embryonic transcripts appear, marking the passage from maternal to embryonic control of development. Some of these genes have been proved to be regulated by a zygotic clock which is set upon egg-activation at fertilization. Following treatment of 1-cell embryos with aphidicolin and cytochalasin D inhibitors of DNA replication and cytokinesis, respectively, here we show that Xist expression is dependent on first and second round of DNA replications and is regulated by a zygotic clock.

Original languageEnglish
Pages (from-to)145-153
Number of pages9
JournalRendiconti Lincei
Volume9
Issue number2
Publication statusPublished - 1998

Keywords

  • Gene expression
  • Imprinting
  • Preimplantation development
  • X-inactivation
  • Xist

ASJC Scopus subject areas

  • Engineering(all)

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