Abstract
Two different optical fiber probes for combined Raman and fluorescence spectroscopic measurements were designed, developed and used for tissue diagnostics. Two visible laser diodes were used for fluorescence spectroscopy, whereas a laser diode emitting in the NIR was used for Raman spectroscopy. The two probes were based on fiber bundles with a central multimode optical fiber, used for delivering light to the tissue, and 24 surrounding optical fibers for signal collection. Both fluorescence and Raman spectra were acquired using the same detection unit, based on a cooled CCD camera, connected to a spectrograph. The two probes were successfully employed for diagnostic purposes on various tissues in a good agreement with common routine histology. This study included skin, brain and bladder tissues and in particular the classification of: malignant melanoma against melanocytic lesions and healthy skin; urothelial carcinoma against healthy bladder mucosa; brain tumor against dysplastic brain tissue. The diagnostic capabilities were determined using a cross-validation method with a leave-one-out approach, finding very high sensitivity and specificity for all the examined tissues. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities. The system presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used for endoscopic inspections in the near future.
| Original language | English |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Publisher | SPIE |
| Volume | 9537 |
| ISBN (Print) | 9781628417029 |
| DOIs | |
| Publication status | Published - 2015 |
| Event | Clinical and Biomedical Spectroscopy and Imaging IV - Munich, Germany Duration: Jun 22 2015 → Jun 24 2015 |
Other
| Other | Clinical and Biomedical Spectroscopy and Imaging IV |
|---|---|
| Country | Germany |
| City | Munich |
| Period | 6/22/15 → 6/24/15 |
Fingerprint
Keywords
- (170.6935) Tissue characterization
- (300.6280) Spectroscopy
- (300.6450) Spectroscopy
- fluorescence and luminescence
- Raman
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Radiology Nuclear Medicine and imaging
Cite this
Tissue classification and diagnostics using a fiber probe for combined Raman and fluorescence spectroscopy. / Cicchi, Riccardo; Anand, Suresh; Crisci, Alfonso; Giordano, Flavio; Rossari, Susanna; De Giorgi, Vincenzo; Maio, Vincenza; Massi, Daniela; Nesi, Gabriella; Buccoliero, Anna Maria; Guerrini, Renzo; Pimpinelli, Nicola; Pavone, Francesco S.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9537 SPIE, 2015. 95370I.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Tissue classification and diagnostics using a fiber probe for combined Raman and fluorescence spectroscopy
AU - Cicchi, Riccardo
AU - Anand, Suresh
AU - Crisci, Alfonso
AU - Giordano, Flavio
AU - Rossari, Susanna
AU - De Giorgi, Vincenzo
AU - Maio, Vincenza
AU - Massi, Daniela
AU - Nesi, Gabriella
AU - Buccoliero, Anna Maria
AU - Guerrini, Renzo
AU - Pimpinelli, Nicola
AU - Pavone, Francesco S.
PY - 2015
Y1 - 2015
N2 - Two different optical fiber probes for combined Raman and fluorescence spectroscopic measurements were designed, developed and used for tissue diagnostics. Two visible laser diodes were used for fluorescence spectroscopy, whereas a laser diode emitting in the NIR was used for Raman spectroscopy. The two probes were based on fiber bundles with a central multimode optical fiber, used for delivering light to the tissue, and 24 surrounding optical fibers for signal collection. Both fluorescence and Raman spectra were acquired using the same detection unit, based on a cooled CCD camera, connected to a spectrograph. The two probes were successfully employed for diagnostic purposes on various tissues in a good agreement with common routine histology. This study included skin, brain and bladder tissues and in particular the classification of: malignant melanoma against melanocytic lesions and healthy skin; urothelial carcinoma against healthy bladder mucosa; brain tumor against dysplastic brain tissue. The diagnostic capabilities were determined using a cross-validation method with a leave-one-out approach, finding very high sensitivity and specificity for all the examined tissues. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities. The system presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used for endoscopic inspections in the near future.
AB - Two different optical fiber probes for combined Raman and fluorescence spectroscopic measurements were designed, developed and used for tissue diagnostics. Two visible laser diodes were used for fluorescence spectroscopy, whereas a laser diode emitting in the NIR was used for Raman spectroscopy. The two probes were based on fiber bundles with a central multimode optical fiber, used for delivering light to the tissue, and 24 surrounding optical fibers for signal collection. Both fluorescence and Raman spectra were acquired using the same detection unit, based on a cooled CCD camera, connected to a spectrograph. The two probes were successfully employed for diagnostic purposes on various tissues in a good agreement with common routine histology. This study included skin, brain and bladder tissues and in particular the classification of: malignant melanoma against melanocytic lesions and healthy skin; urothelial carcinoma against healthy bladder mucosa; brain tumor against dysplastic brain tissue. The diagnostic capabilities were determined using a cross-validation method with a leave-one-out approach, finding very high sensitivity and specificity for all the examined tissues. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities. The system presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used for endoscopic inspections in the near future.
KW - (170.6935) Tissue characterization
KW - (300.6280) Spectroscopy
KW - (300.6450) Spectroscopy
KW - fluorescence and luminescence
KW - Raman
UR - http://www.scopus.com/inward/record.url?scp=84939795379&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84939795379&partnerID=8YFLogxK
U2 - 10.1117/12.2183626
DO - 10.1117/12.2183626
M3 - Conference contribution
AN - SCOPUS:84939795379
SN - 9781628417029
VL - 9537
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
PB - SPIE
ER -