Tissue classification and diagnostics using a fiber probe for combined Raman and fluorescence spectroscopy

Riccardo Cicchi, Suresh Anand, Alfonso Crisci, Flavio Giordano, Susanna Rossari, Vincenzo De Giorgi, Vincenza Maio, Daniela Massi, Gabriella Nesi, Anna Maria Buccoliero, Renzo Guerrini, Nicola Pimpinelli, Francesco S. Pavone

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Two different optical fiber probes for combined Raman and fluorescence spectroscopic measurements were designed, developed and used for tissue diagnostics. Two visible laser diodes were used for fluorescence spectroscopy, whereas a laser diode emitting in the NIR was used for Raman spectroscopy. The two probes were based on fiber bundles with a central multimode optical fiber, used for delivering light to the tissue, and 24 surrounding optical fibers for signal collection. Both fluorescence and Raman spectra were acquired using the same detection unit, based on a cooled CCD camera, connected to a spectrograph. The two probes were successfully employed for diagnostic purposes on various tissues in a good agreement with common routine histology. This study included skin, brain and bladder tissues and in particular the classification of: malignant melanoma against melanocytic lesions and healthy skin; urothelial carcinoma against healthy bladder mucosa; brain tumor against dysplastic brain tissue. The diagnostic capabilities were determined using a cross-validation method with a leave-one-out approach, finding very high sensitivity and specificity for all the examined tissues. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities. The system presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used for endoscopic inspections in the near future.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISBN (Print)9781628417029
Publication statusPublished - 2015
EventClinical and Biomedical Spectroscopy and Imaging IV - Munich, Germany
Duration: Jun 22 2015Jun 24 2015


OtherClinical and Biomedical Spectroscopy and Imaging IV


  • (170.6935) Tissue characterization
  • (300.6280) Spectroscopy
  • (300.6450) Spectroscopy
  • fluorescence and luminescence
  • Raman

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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