Tissue Culture Society Congress, Brno, April 21-24, 1996. Cytokine-based gene therapy of human tumors. An overview

G. Parmiani, F. Arienti, J. Sule-Suso, C. Melani, M. P. Colombo, V. Ramakrishna, F. Belli, L. Mascheroni, L. Rivoltini, N. Cascinelli

Research output: Contribution to journalArticlepeer-review

Abstract

This review first summarizes the different strategies of gene therapy of cancer and then focuses on the immunological approach. Several studies in animal models with cytokine gene-transduced tumor cells indicate that local cytokine release usually results in tumor growth inhibition. Moreover, in a number of cases vaccination with such cells can reduce growth of established tumors or even cure the tumor-bearing animals. Translation of such a principle in human clinical setting is reported. We have transduced human melanoma cells with gents coding for interleukin (IL)-2, IL-4 or B7-1 and characterized such lines. The phenotype did not change after gene insertion but the functional, immunostimulatory activity of IL-2 or B7-1 gene-transduced melanoma cells was significantly increased compared to that of parental lines. These lines were then used to vaccinate melanoma patients. Preliminary results of trials with IL-2 gene-transduced cells are presented which indicate a weak clinical response and the activation of a melanoma-specific cytotoxic T lymphocyte response in a low percentage of patients.

Original languageEnglish
Pages (from-to)305-309
Number of pages5
JournalFolia Biologica
Volume42
Issue number6
Publication statusPublished - 1996

Keywords

  • Cytokines
  • Gene therapy
  • Tumors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

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