Abstract
Crinoid echinoderms can completely and rapidly regenerate arms lost following self-induced or traumatic amputation. Arm regeneration in these animals therefore provides a valuable experimental model for studying all aspects of regenerative processes, particularly with respect to the nervous system and its specific contribution to regenerative phenomena. Taking into account the primary role of the nervous system in regeneration in other invertebrates, we have investigated the specific involvement of neural factors, viz. the monoamine neurotransmitters dopamine and serotonin, in arm regeneration of Antedon mediterranea. In the present work, the presence of classical monoamines has been revealed by employing specific immunocytochemical and histofluorescence tests in association with biochemical detection by means of high pressure liquid chromatography. The distribution pattern of these neurohumoral molecules at standard regenerative stages has been compared with that of normal non-regenerating arms. Results indicate that both dopamine and serotonin dramatically change in both their distribution and concentration during the repair and regenerative processes. Their remarkably enhanced pattern during regeneration and widespread presence at the level of both nervous and non-nervous tissues indicates that they are important neural growth-promoting factors in crinoid arm regeneration.
| Original language | English |
|---|---|
| Pages (from-to) | 341-352 |
| Number of pages | 12 |
| Journal | Cell & Tissue Research |
| Volume | 285 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1996 |
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Keywords
- Antedon mediterranea (Echinodermata)
- Arm regeneration
- Dopamine
- Nervous system
- Serotonin
ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Anatomy
Cite this
Tissue distribution of monoamine neurotransmitters in normal and regenerating arms of the feather star Antedon mediterranea. / Carnevali, M. Daniela Candia; Bonasoro, Francesco; Invernizzi, Roberto; Lucca, Elisa; Welsch, Ulrich; Thorndyke, Michael C.
In: Cell & Tissue Research, Vol. 285, No. 2, 1996, p. 341-352.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tissue distribution of monoamine neurotransmitters in normal and regenerating arms of the feather star Antedon mediterranea
AU - Carnevali, M. Daniela Candia
AU - Bonasoro, Francesco
AU - Invernizzi, Roberto
AU - Lucca, Elisa
AU - Welsch, Ulrich
AU - Thorndyke, Michael C.
PY - 1996
Y1 - 1996
N2 - Crinoid echinoderms can completely and rapidly regenerate arms lost following self-induced or traumatic amputation. Arm regeneration in these animals therefore provides a valuable experimental model for studying all aspects of regenerative processes, particularly with respect to the nervous system and its specific contribution to regenerative phenomena. Taking into account the primary role of the nervous system in regeneration in other invertebrates, we have investigated the specific involvement of neural factors, viz. the monoamine neurotransmitters dopamine and serotonin, in arm regeneration of Antedon mediterranea. In the present work, the presence of classical monoamines has been revealed by employing specific immunocytochemical and histofluorescence tests in association with biochemical detection by means of high pressure liquid chromatography. The distribution pattern of these neurohumoral molecules at standard regenerative stages has been compared with that of normal non-regenerating arms. Results indicate that both dopamine and serotonin dramatically change in both their distribution and concentration during the repair and regenerative processes. Their remarkably enhanced pattern during regeneration and widespread presence at the level of both nervous and non-nervous tissues indicates that they are important neural growth-promoting factors in crinoid arm regeneration.
AB - Crinoid echinoderms can completely and rapidly regenerate arms lost following self-induced or traumatic amputation. Arm regeneration in these animals therefore provides a valuable experimental model for studying all aspects of regenerative processes, particularly with respect to the nervous system and its specific contribution to regenerative phenomena. Taking into account the primary role of the nervous system in regeneration in other invertebrates, we have investigated the specific involvement of neural factors, viz. the monoamine neurotransmitters dopamine and serotonin, in arm regeneration of Antedon mediterranea. In the present work, the presence of classical monoamines has been revealed by employing specific immunocytochemical and histofluorescence tests in association with biochemical detection by means of high pressure liquid chromatography. The distribution pattern of these neurohumoral molecules at standard regenerative stages has been compared with that of normal non-regenerating arms. Results indicate that both dopamine and serotonin dramatically change in both their distribution and concentration during the repair and regenerative processes. Their remarkably enhanced pattern during regeneration and widespread presence at the level of both nervous and non-nervous tissues indicates that they are important neural growth-promoting factors in crinoid arm regeneration.
KW - Antedon mediterranea (Echinodermata)
KW - Arm regeneration
KW - Dopamine
KW - Nervous system
KW - Serotonin
UR - http://www.scopus.com/inward/record.url?scp=0029884405&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029884405&partnerID=8YFLogxK
U2 - 10.1007/s004410050651
DO - 10.1007/s004410050651
M3 - Article
AN - SCOPUS:0029884405
VL - 285
SP - 341
EP - 352
JO - Cell and Tissue Research
JF - Cell and Tissue Research
SN - 0302-766X
IS - 2
ER -