TY - JOUR
T1 - Tissue engineered esophageal patch by mesenchymal stromal cells
T2 - Optimization of electrospun patch engineering
AU - Pisani, Silvia
AU - Croce, Stefania
AU - Chiesa, Enrica
AU - Dorati, Rossella
AU - Lenta, Elisa
AU - Genta, Ida
AU - Bruni, Giovanna
AU - Mauramati, Simone
AU - Benazzo, Alberto
AU - Cobianchi, Lorenzo
AU - Morbini, Patrizia
AU - Caliogna, Laura
AU - Benazzo, Marco
AU - Avanzini, Maria Antonietta
AU - Conti, Bice
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Aim of work was to locate a simple, reproducible protocol for uniform seeding and optimal cellularization of biodegradable patch minimizing the risk of structural damages of patch and its contamination in long-term culture. Two seeding procedures are exploited, namely static seeding procedures on biodegradable and biocompatible patches incubated as free floating (floating conditions) or supported by CellCrown™ insert (fixed conditions) and engineered by porcine bone marrow MSCs (p-MSCs). Scaffold prototypes having specific structural features with regard to pore size, pore orientation, porosity, and pore distribution were produced using two different techniques, such as temperature-induced precipitation method and electrospinning technology. The investigation on different prototypes allowed achieving several implementations in terms of cell distribution uniformity, seeding efficiency, and cellularization timing. The cell seeding protocol in stating conditions demonstrated to be the most suitable method, as these conditions successfully improved the cellularization of polymeric patches. Furthermore, the investigation provided interesting information on patches’ stability in physiological simulating experimental conditions. Considering the in vitro results, it can be stated that the in vitro protocol proposed for patches cellularization is suitable to achieve homogeneous and complete cellularizations of patch. Moreover, the protocol turned out to be simple, repeatable, and reproducible.
AB - Aim of work was to locate a simple, reproducible protocol for uniform seeding and optimal cellularization of biodegradable patch minimizing the risk of structural damages of patch and its contamination in long-term culture. Two seeding procedures are exploited, namely static seeding procedures on biodegradable and biocompatible patches incubated as free floating (floating conditions) or supported by CellCrown™ insert (fixed conditions) and engineered by porcine bone marrow MSCs (p-MSCs). Scaffold prototypes having specific structural features with regard to pore size, pore orientation, porosity, and pore distribution were produced using two different techniques, such as temperature-induced precipitation method and electrospinning technology. The investigation on different prototypes allowed achieving several implementations in terms of cell distribution uniformity, seeding efficiency, and cellularization timing. The cell seeding protocol in stating conditions demonstrated to be the most suitable method, as these conditions successfully improved the cellularization of polymeric patches. Furthermore, the investigation provided interesting information on patches’ stability in physiological simulating experimental conditions. Considering the in vitro results, it can be stated that the in vitro protocol proposed for patches cellularization is suitable to achieve homogeneous and complete cellularizations of patch. Moreover, the protocol turned out to be simple, repeatable, and reproducible.
KW - Electrospinning
KW - Patch engineering
KW - Porcine mesenchymal stem cells
KW - Temperature induced precipitation
UR - http://www.scopus.com/inward/record.url?scp=85081030791&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85081030791&partnerID=8YFLogxK
U2 - 10.3390/ijms21051764
DO - 10.3390/ijms21051764
M3 - Article
AN - SCOPUS:85081030791
VL - 21
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 5
M1 - 1764
ER -