Tissue engineered esophageal patch by mesenchymal stromal cells: Optimization of electrospun patch engineering

Silvia Pisani, Stefania Croce, Enrica Chiesa, Rossella Dorati, Elisa Lenta, Ida Genta, Giovanna Bruni, Simone Mauramati, Alberto Benazzo, Lorenzo Cobianchi, Patrizia Morbini, Laura Caliogna, Marco Benazzo, Maria Antonietta Avanzini, Bice Conti

Research output: Contribution to journalArticle

Abstract

Aim of work was to locate a simple, reproducible protocol for uniform seeding and optimal cellularization of biodegradable patch minimizing the risk of structural damages of patch and its contamination in long-term culture. Two seeding procedures are exploited, namely static seeding procedures on biodegradable and biocompatible patches incubated as free floating (floating conditions) or supported by CellCrown™ insert (fixed conditions) and engineered by porcine bone marrow MSCs (p-MSCs). Scaffold prototypes having specific structural features with regard to pore size, pore orientation, porosity, and pore distribution were produced using two different techniques, such as temperature-induced precipitation method and electrospinning technology. The investigation on different prototypes allowed achieving several implementations in terms of cell distribution uniformity, seeding efficiency, and cellularization timing. The cell seeding protocol in stating conditions demonstrated to be the most suitable method, as these conditions successfully improved the cellularization of polymeric patches. Furthermore, the investigation provided interesting information on patches’ stability in physiological simulating experimental conditions. Considering the in vitro results, it can be stated that the in vitro protocol proposed for patches cellularization is suitable to achieve homogeneous and complete cellularizations of patch. Moreover, the protocol turned out to be simple, repeatable, and reproducible.

Original languageEnglish
Article number1764
JournalInternational Journal of Molecular Sciences
Volume21
Issue number5
DOIs
Publication statusPublished - Mar 1 2020

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Keywords

  • Electrospinning
  • Patch engineering
  • Porcine mesenchymal stem cells
  • Temperature induced precipitation

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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