Tissue engineering for cartilage repair: in vitro properties of a hyaluronan-derivative.

B. Grigolo, G. Lisignoli, A. Piacentini, M. Fiorini, L. Roseti, L. De Franceschi, E. Tognana, A. Pavesio, A. Facchini

Research output: Contribution to journalArticle

Abstract

Association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage and bone repair. Such scaffolds should provide a performed three-dimensional shape, prevent cells from floating out of the defect, have sufficient mechanical strength, facilitate uniform spread of cells, and stimulate the phenotype of transplanted cells. Hyaff-11 is a recently developed hyaluronic-acid based biodegradable polymer, that has been shown to provide successful cell scaffolds for tissue-engineered repair. The aim of this study was to evaluate in vitro the potential of Hyaff-11 to support the growth of human chondrocytes and to maintain their original phenotype. Our data indicate that human chondrocytes seeded on Hyaff-11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I. These results provide an in vitro demonstration of therapeutic potential of Hyaff-11 as a delivery vehicle in tissue-engineered repair of articular cartilage defects.

Original languageEnglish
Pages (from-to)351-355
Number of pages5
JournalLa Chirurgia degli organi di movimento
Volume88
Issue number4
Publication statusPublished - 2003

Fingerprint

Hyaluronic Acid
Tissue Engineering
Cartilage
Chondrocytes
Tissue Scaffolds
Phenotype
Aggrecans
Collagen Type II
Cell Shape
Articular Cartilage
Biocompatible Materials
Collagen Type I
Polymers
Down-Regulation
Bone and Bones
Equipment and Supplies
In Vitro Techniques
Growth
Therapeutics

Cite this

Tissue engineering for cartilage repair : in vitro properties of a hyaluronan-derivative. / Grigolo, B.; Lisignoli, G.; Piacentini, A.; Fiorini, M.; Roseti, L.; De Franceschi, L.; Tognana, E.; Pavesio, A.; Facchini, A.

In: La Chirurgia degli organi di movimento, Vol. 88, No. 4, 2003, p. 351-355.

Research output: Contribution to journalArticle

Grigolo, B, Lisignoli, G, Piacentini, A, Fiorini, M, Roseti, L, De Franceschi, L, Tognana, E, Pavesio, A & Facchini, A 2003, 'Tissue engineering for cartilage repair: in vitro properties of a hyaluronan-derivative.', La Chirurgia degli organi di movimento, vol. 88, no. 4, pp. 351-355.
Grigolo, B. ; Lisignoli, G. ; Piacentini, A. ; Fiorini, M. ; Roseti, L. ; De Franceschi, L. ; Tognana, E. ; Pavesio, A. ; Facchini, A. / Tissue engineering for cartilage repair : in vitro properties of a hyaluronan-derivative. In: La Chirurgia degli organi di movimento. 2003 ; Vol. 88, No. 4. pp. 351-355.
@article{c5a1656208144e688c621bd63a5321bd,
title = "Tissue engineering for cartilage repair: in vitro properties of a hyaluronan-derivative.",
abstract = "Association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage and bone repair. Such scaffolds should provide a performed three-dimensional shape, prevent cells from floating out of the defect, have sufficient mechanical strength, facilitate uniform spread of cells, and stimulate the phenotype of transplanted cells. Hyaff-11 is a recently developed hyaluronic-acid based biodegradable polymer, that has been shown to provide successful cell scaffolds for tissue-engineered repair. The aim of this study was to evaluate in vitro the potential of Hyaff-11 to support the growth of human chondrocytes and to maintain their original phenotype. Our data indicate that human chondrocytes seeded on Hyaff-11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I. These results provide an in vitro demonstration of therapeutic potential of Hyaff-11 as a delivery vehicle in tissue-engineered repair of articular cartilage defects.",
author = "B. Grigolo and G. Lisignoli and A. Piacentini and M. Fiorini and L. Roseti and {De Franceschi}, L. and E. Tognana and A. Pavesio and A. Facchini",
year = "2003",
language = "English",
volume = "88",
pages = "351--355",
journal = "Chirurgia degli Organi di Movimento",
issn = "0009-4749",
publisher = "Nouva Casa Edtrice Licino Cappelli GEM",
number = "4",

}

TY - JOUR

T1 - Tissue engineering for cartilage repair

T2 - in vitro properties of a hyaluronan-derivative.

AU - Grigolo, B.

AU - Lisignoli, G.

AU - Piacentini, A.

AU - Fiorini, M.

AU - Roseti, L.

AU - De Franceschi, L.

AU - Tognana, E.

AU - Pavesio, A.

AU - Facchini, A.

PY - 2003

Y1 - 2003

N2 - Association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage and bone repair. Such scaffolds should provide a performed three-dimensional shape, prevent cells from floating out of the defect, have sufficient mechanical strength, facilitate uniform spread of cells, and stimulate the phenotype of transplanted cells. Hyaff-11 is a recently developed hyaluronic-acid based biodegradable polymer, that has been shown to provide successful cell scaffolds for tissue-engineered repair. The aim of this study was to evaluate in vitro the potential of Hyaff-11 to support the growth of human chondrocytes and to maintain their original phenotype. Our data indicate that human chondrocytes seeded on Hyaff-11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I. These results provide an in vitro demonstration of therapeutic potential of Hyaff-11 as a delivery vehicle in tissue-engineered repair of articular cartilage defects.

AB - Association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage and bone repair. Such scaffolds should provide a performed three-dimensional shape, prevent cells from floating out of the defect, have sufficient mechanical strength, facilitate uniform spread of cells, and stimulate the phenotype of transplanted cells. Hyaff-11 is a recently developed hyaluronic-acid based biodegradable polymer, that has been shown to provide successful cell scaffolds for tissue-engineered repair. The aim of this study was to evaluate in vitro the potential of Hyaff-11 to support the growth of human chondrocytes and to maintain their original phenotype. Our data indicate that human chondrocytes seeded on Hyaff-11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I. These results provide an in vitro demonstration of therapeutic potential of Hyaff-11 as a delivery vehicle in tissue-engineered repair of articular cartilage defects.

UR - http://www.scopus.com/inward/record.url?scp=16544369919&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16544369919&partnerID=8YFLogxK

M3 - Article

C2 - 15259550

AN - SCOPUS:16544369919

VL - 88

SP - 351

EP - 355

JO - Chirurgia degli Organi di Movimento

JF - Chirurgia degli Organi di Movimento

SN - 0009-4749

IS - 4

ER -