TY - JOUR
T1 - Tissue engineering for total meniscal substitution
T2 - Animal study in sheep model-results at 12 months
AU - Kon, Elizaveta
AU - Filardo, Giuseppe
AU - Tschon, Matilde
AU - Fini, Milena
AU - Giavaresi, Gianluca
AU - Reggiani, Leonardo Marchesini
AU - Chiari, Catharina
AU - Nehrer, Stefan
AU - Martin, Ivan
AU - Salter, Donald M.
AU - Ambrosio, Luigi
AU - Marcacci, Maurilio
PY - 2012/8/1
Y1 - 2012/8/1
N2 - The aim of the study was to investigate the use of a hyaluronic acid/polycaprolactone material for meniscal tissue engineering and to evaluate the tissue regeneration after the augmentation of the implant with expanded autologous chondrocytes. Eighteen skeletally mature sheep were treated. The animals were divided into three groups: cell-free scaffold, scaffold seeded with autologous chondrocytes, and meniscectomy alone. The implant was sutured to the capsule and to the meniscal ligament. At a 12-month gross assessment, histology and histomorphometry were used to assess the meniscus implant, knee joint, and osteoarthritis development. All implants showed excellent capsular ingrowth at the periphery. The implant gross assessment showed significant differences between cell-seeded and cell-free groups (p=0.011). The histological analysis indicated a cellular colonization throughout the implanted constructs. Avascular cartilaginous tissue formation was significantly more frequent in the cell-seeded constructs. Joint gross assessment showed that sheep treated with scaffold implantation achieved a significant higher score than those underwent meniscectomy (p
AB - The aim of the study was to investigate the use of a hyaluronic acid/polycaprolactone material for meniscal tissue engineering and to evaluate the tissue regeneration after the augmentation of the implant with expanded autologous chondrocytes. Eighteen skeletally mature sheep were treated. The animals were divided into three groups: cell-free scaffold, scaffold seeded with autologous chondrocytes, and meniscectomy alone. The implant was sutured to the capsule and to the meniscal ligament. At a 12-month gross assessment, histology and histomorphometry were used to assess the meniscus implant, knee joint, and osteoarthritis development. All implants showed excellent capsular ingrowth at the periphery. The implant gross assessment showed significant differences between cell-seeded and cell-free groups (p=0.011). The histological analysis indicated a cellular colonization throughout the implanted constructs. Avascular cartilaginous tissue formation was significantly more frequent in the cell-seeded constructs. Joint gross assessment showed that sheep treated with scaffold implantation achieved a significant higher score than those underwent meniscectomy (p
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U2 - 10.1089/ten.tea.2011.0572
DO - 10.1089/ten.tea.2011.0572
M3 - Article
C2 - 22500654
AN - SCOPUS:84865275513
VL - 18
SP - 1573
EP - 1582
JO - Tissue Engineering - Part A.
JF - Tissue Engineering - Part A.
SN - 1937-3341
IS - 15-16
ER -