Tissue factor and CCL2/monocyte chemoattractant protein-1 released by human islets affect islet engraftment in type 1 diabetic recipients

Federico Bertuzzi, Simona Marzorati, Paola Maffi, Lorenzo Piemonti, Raffaella Melzi, Francesca De Taddeo, Veronica Valtolina, Armando D'angelo, Valerio Di Carlo, Ezio Bonifacio, Antonio Secchi

Research output: Contribution to journalArticlepeer-review

Abstract

Islet survival in the early posttransplantation period is likely to be influenced by inflammatory events in and around islets. Twenty-seven human islet preparations were transplanted by 24 infusions into 14 patients with brittle type 1 diabetes under the Edmonton protocol. Patients were monitored for their coagulation [cross-linked fibrin degradation products (XDPs)] and liver function test [aspartate and alanine aminotransferase (AST and ALT)] as markers of early posttransplant complications, and these were correlated with in vitro islet number, purification, volume, monocyte-chemoattractant protein-1 (CCL2/MCP-1) and tissue factor (TF) islet release. Consistent with activation of coagulation pathways and hepatic damage, serum XDP values increased early after 11 infusions and transaminase after 13 of 24 infusions. TF and CCL2/MCP-1 were detected in supernatant s of 21 and 22 islet preparations, respectively. Seram XDP peak values were correlated with TF/equivalent islets (EI) (r2=0.26, P = 0.001) and CCL2/MCP-1/EI (r2 = 0.42; P <0.001); serum transaminase areas under the curve in the first week posttransplantation were correlated with CCL2/MCP-1/EI (r2 = 0.55; P <0.001 for ALT and r2 = 0.51; P = 0.001 for AST) and TF/EI (r2 = 0.31; P = 0.002 for ALT, and r2 = 0.36; P = 0.002 for AST). These data suggest that reducing the islet proinflammatory state may be a means to reduce the early posttransplant complications and perhaps improve islet engraftment.

Original languageEnglish
Pages (from-to)5724-5728
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume89
Issue number11
DOIs
Publication statusPublished - Nov 2004

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

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