Tissue plasminogen activator and urokinase plasminogen activator in human epileptogenic pathologies

A. M. Iyer, E. Zurolo, K. Boer, J. C. Baayen, F. Giangaspero, A. Arcella, G. C. Di Gennaro, V. Esposito, W. G M Spliet, P. C. van Rijen, D. Troost, J. A. Gorter, E. Aronica

Research output: Contribution to journalArticlepeer-review

Abstract

A growing body of evidence demonstrates the involvement of plasminogen activators (PAs) in a number of physiologic and pathologic events in the CNS. Induction of both tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) has been observed in different experimental models of epilepsy and tPA has been implicated in the mechanisms underlying seizure activity. We investigated the expression and the cellular distribution of tPA and uPA in several epileptogenic pathologies, including hippocampal sclerosis (HS; n=6), and developmental glioneuronal lesions, such as focal cortical dysplasia (FCD, n=6), cortical tubers in patients with the tuberous sclerosis complex (TSC; n=6) and in gangliogliomas (GG; n=6), using immuno-cytochemical, western blot and real-time quantitative PCR analysis. TPA and uPA immunostaining showed increased expression within the epileptogenic lesions compared to control specimens in both glial and neuronal cells (hippocampal neurons in HS and dysplastic neurons in FCD, TSC and GG specimens). Confocal laser scanning microscopy confirmed expression of both proteins in astrocytes and microglia, as well as in microvascular endothelium. Immunoblot demonstrated also up-regulation of the uPA receptor (uPAR; P

Original languageEnglish
Pages (from-to)929-945
Number of pages17
JournalNeuroscience
Volume167
Issue number3
DOIs
Publication statusPublished - May 2010

Keywords

  • Epilepsy
  • Focal cortical dysplasia
  • Ganglioglioma
  • Hippocampal sclerosis
  • Tuberous sclerosis

ASJC Scopus subject areas

  • Neuroscience(all)

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