Tissue properties of the human vertebral body sub-structures evaluated by means of microindentation

E. Dall'Ara, C. Karl, G. Mazza, G. Franzoso, P. Vena, M. Pretterklieber, D. Pahr, P. Zysset

Research output: Contribution to journalArticle

Abstract

Purpose: The better understanding of vertebral mechanical properties can help to improve the diagnosis of vertebral fractures. As the bone mechanical competence depends not only from bone mineral density (BMD) but also from bone quality, the goal of the present study was to investigate the anisotropic indentation moduli of the different sub-structures of the healthy human vertebral body and spondylophytes by means of microindentation. Methods: Six human vertebral bodies and five osteophytes (spondylophytes) were collected and prepared for microindentation test. In particular, indentations were performed on bone structural units of the cortical shell (along axial, circumferential and radial directions), of the endplates (along the anterio-posterior and lateral directions), of the trabecular bone (along the axial and transverse directions) and of the spondylophytes (along the axial direction). A total of 3164 indentations down to a maximum depth of 2.5. μm were performed and the indentation modulus was computed for each measurement. Results: The cortical shell showed an orthotropic behavior (indentation modulus, Ei, higher if measured along the axial direction, 14.6±2.8. GPa, compared to the circumferential one, 12.3±3.5. GPa, and radial one, 8.3±3.1. GPa). Moreover, the cortical endplates (similar Ei along the antero-posterior, 13.0±2.9. GPa, and along the lateral, 12.0±3.0. GPa, directions) and the trabecular bone (Ei= 13.7±3.4. GPa along the axial direction versus Ei=10.9±3.7. GPa along the transverse one) showed transversal isotropy behavior. Furthermore, the spondylophytes showed the lower mechanical properties measured along the axial direction (Ei=10.5±3.3. GPa). Conclusions: The original results presented in this study improve our understanding of vertebral biomechanics and can be helpful to define the material properties of the vertebral substructures in computational models such as FE analysis.

Original languageEnglish
Pages (from-to)23-32
Number of pages10
JournalJournal of the Mechanical Behavior of Biomedical Materials
Volume25
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Human Body
Bone
Indentation
Tissue
Bone and Bones
Mechanical properties
Biomechanics
Osteophyte
Direction compound
Materials properties
Minerals
Biomechanical Phenomena
Bone Density
Mental Competency

Keywords

  • Anisotropy
  • Cortical bone
  • Human vertebra
  • Microindentation
  • Osteophytes
  • Trabecular bone

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Mechanics of Materials
  • Medicine(all)

Cite this

Tissue properties of the human vertebral body sub-structures evaluated by means of microindentation. / Dall'Ara, E.; Karl, C.; Mazza, G.; Franzoso, G.; Vena, P.; Pretterklieber, M.; Pahr, D.; Zysset, P.

In: Journal of the Mechanical Behavior of Biomedical Materials, Vol. 25, 09.2013, p. 23-32.

Research output: Contribution to journalArticle

Dall'Ara, E. ; Karl, C. ; Mazza, G. ; Franzoso, G. ; Vena, P. ; Pretterklieber, M. ; Pahr, D. ; Zysset, P. / Tissue properties of the human vertebral body sub-structures evaluated by means of microindentation. In: Journal of the Mechanical Behavior of Biomedical Materials. 2013 ; Vol. 25. pp. 23-32.
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abstract = "Purpose: The better understanding of vertebral mechanical properties can help to improve the diagnosis of vertebral fractures. As the bone mechanical competence depends not only from bone mineral density (BMD) but also from bone quality, the goal of the present study was to investigate the anisotropic indentation moduli of the different sub-structures of the healthy human vertebral body and spondylophytes by means of microindentation. Methods: Six human vertebral bodies and five osteophytes (spondylophytes) were collected and prepared for microindentation test. In particular, indentations were performed on bone structural units of the cortical shell (along axial, circumferential and radial directions), of the endplates (along the anterio-posterior and lateral directions), of the trabecular bone (along the axial and transverse directions) and of the spondylophytes (along the axial direction). A total of 3164 indentations down to a maximum depth of 2.5. μm were performed and the indentation modulus was computed for each measurement. Results: The cortical shell showed an orthotropic behavior (indentation modulus, Ei, higher if measured along the axial direction, 14.6±2.8. GPa, compared to the circumferential one, 12.3±3.5. GPa, and radial one, 8.3±3.1. GPa). Moreover, the cortical endplates (similar Ei along the antero-posterior, 13.0±2.9. GPa, and along the lateral, 12.0±3.0. GPa, directions) and the trabecular bone (Ei= 13.7±3.4. GPa along the axial direction versus Ei=10.9±3.7. GPa along the transverse one) showed transversal isotropy behavior. Furthermore, the spondylophytes showed the lower mechanical properties measured along the axial direction (Ei=10.5±3.3. GPa). Conclusions: The original results presented in this study improve our understanding of vertebral biomechanics and can be helpful to define the material properties of the vertebral substructures in computational models such as FE analysis.",
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T1 - Tissue properties of the human vertebral body sub-structures evaluated by means of microindentation

AU - Dall'Ara, E.

AU - Karl, C.

AU - Mazza, G.

AU - Franzoso, G.

AU - Vena, P.

AU - Pretterklieber, M.

AU - Pahr, D.

AU - Zysset, P.

PY - 2013/9

Y1 - 2013/9

N2 - Purpose: The better understanding of vertebral mechanical properties can help to improve the diagnosis of vertebral fractures. As the bone mechanical competence depends not only from bone mineral density (BMD) but also from bone quality, the goal of the present study was to investigate the anisotropic indentation moduli of the different sub-structures of the healthy human vertebral body and spondylophytes by means of microindentation. Methods: Six human vertebral bodies and five osteophytes (spondylophytes) were collected and prepared for microindentation test. In particular, indentations were performed on bone structural units of the cortical shell (along axial, circumferential and radial directions), of the endplates (along the anterio-posterior and lateral directions), of the trabecular bone (along the axial and transverse directions) and of the spondylophytes (along the axial direction). A total of 3164 indentations down to a maximum depth of 2.5. μm were performed and the indentation modulus was computed for each measurement. Results: The cortical shell showed an orthotropic behavior (indentation modulus, Ei, higher if measured along the axial direction, 14.6±2.8. GPa, compared to the circumferential one, 12.3±3.5. GPa, and radial one, 8.3±3.1. GPa). Moreover, the cortical endplates (similar Ei along the antero-posterior, 13.0±2.9. GPa, and along the lateral, 12.0±3.0. GPa, directions) and the trabecular bone (Ei= 13.7±3.4. GPa along the axial direction versus Ei=10.9±3.7. GPa along the transverse one) showed transversal isotropy behavior. Furthermore, the spondylophytes showed the lower mechanical properties measured along the axial direction (Ei=10.5±3.3. GPa). Conclusions: The original results presented in this study improve our understanding of vertebral biomechanics and can be helpful to define the material properties of the vertebral substructures in computational models such as FE analysis.

AB - Purpose: The better understanding of vertebral mechanical properties can help to improve the diagnosis of vertebral fractures. As the bone mechanical competence depends not only from bone mineral density (BMD) but also from bone quality, the goal of the present study was to investigate the anisotropic indentation moduli of the different sub-structures of the healthy human vertebral body and spondylophytes by means of microindentation. Methods: Six human vertebral bodies and five osteophytes (spondylophytes) were collected and prepared for microindentation test. In particular, indentations were performed on bone structural units of the cortical shell (along axial, circumferential and radial directions), of the endplates (along the anterio-posterior and lateral directions), of the trabecular bone (along the axial and transverse directions) and of the spondylophytes (along the axial direction). A total of 3164 indentations down to a maximum depth of 2.5. μm were performed and the indentation modulus was computed for each measurement. Results: The cortical shell showed an orthotropic behavior (indentation modulus, Ei, higher if measured along the axial direction, 14.6±2.8. GPa, compared to the circumferential one, 12.3±3.5. GPa, and radial one, 8.3±3.1. GPa). Moreover, the cortical endplates (similar Ei along the antero-posterior, 13.0±2.9. GPa, and along the lateral, 12.0±3.0. GPa, directions) and the trabecular bone (Ei= 13.7±3.4. GPa along the axial direction versus Ei=10.9±3.7. GPa along the transverse one) showed transversal isotropy behavior. Furthermore, the spondylophytes showed the lower mechanical properties measured along the axial direction (Ei=10.5±3.3. GPa). Conclusions: The original results presented in this study improve our understanding of vertebral biomechanics and can be helpful to define the material properties of the vertebral substructures in computational models such as FE analysis.

KW - Anisotropy

KW - Cortical bone

KW - Human vertebra

KW - Microindentation

KW - Osteophytes

KW - Trabecular bone

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