Tissue staining for Helicobacter pylori in intestinal metaplasia

Correlation with its extension and histochemical subtypes

P. Testoni, E. Colombo, R. Scelsi, L. Cattani, F. Bagnolo, F. Lella, M. Buizza, O. Luinetti

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The role played by Helicobacter pylori (Hp) infection in the occurrence of non-cardial gastric adenocarcinoma is suggestive but still debated. This study aimed to evaluate: a) the prevalence of Helicobacter-like organisms in antral bioptic specimens of 291 patients with chronic gastritis with antral atrophy and different subtypes of intestinal metaplasia (IM); b) the presence of a possible different positive tissue staining for the bacteria in the complete and incomplete intestinal metaplasia. Of the 291 patients, 222 cases (76.3%) showed type I IM, 28 cases (9.6%) type II IM and 41 cases (14.1%) type III IM. Helicobacter-like organisms were found in 42.9% of cases and positive tissue staining rate appeared to be inversely related to the extension of IM (58.7% in IM extended in less than 30% of specimens, 30.2% in IM extended between 30% and 60%, 2.7% in IM exceeding 60% of the biopsed area). The inverse correlation between lower positive tissue staining for Helicobacter-like organisms and greater extension of IM was statistically significant (p <0.001). Incomplete metaplasia appeared to be unrelated to age and associated with a lower positive tissue staining for Helicobacter-like organisms; among patients with type I metaplasia, 118/222 showed Hp-positive bioptic specimens, vs 7/69 of types II and III (p <0.001). In conclusion, positive tissue staining for Helicobacter-like organisms appeared to be significantly (p <0.001) higher in type I IM, vs types II and III, and to a lesser extent to metaplastic changes; in addition, when metaplastic changes are not greatly extended (less than 30%), the presence of the bacteria is significantly reduced in specimens with incomplete form of intestinal metaplasia in comparison with the complete one.

Original languageEnglish
Pages (from-to)285-290
Number of pages6
JournalItalian Journal of Gastroenterology
Volume27
Issue number6
Publication statusPublished - 1995

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Metaplasia
Helicobacter pylori
Staining and Labeling
Helicobacter
Bacteria
Helicobacter Infections
Gastritis
Atrophy
Stomach
Adenocarcinoma

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Tissue staining for Helicobacter pylori in intestinal metaplasia : Correlation with its extension and histochemical subtypes. / Testoni, P.; Colombo, E.; Scelsi, R.; Cattani, L.; Bagnolo, F.; Lella, F.; Buizza, M.; Luinetti, O.

In: Italian Journal of Gastroenterology, Vol. 27, No. 6, 1995, p. 285-290.

Research output: Contribution to journalArticle

Testoni, P. ; Colombo, E. ; Scelsi, R. ; Cattani, L. ; Bagnolo, F. ; Lella, F. ; Buizza, M. ; Luinetti, O. / Tissue staining for Helicobacter pylori in intestinal metaplasia : Correlation with its extension and histochemical subtypes. In: Italian Journal of Gastroenterology. 1995 ; Vol. 27, No. 6. pp. 285-290.
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abstract = "The role played by Helicobacter pylori (Hp) infection in the occurrence of non-cardial gastric adenocarcinoma is suggestive but still debated. This study aimed to evaluate: a) the prevalence of Helicobacter-like organisms in antral bioptic specimens of 291 patients with chronic gastritis with antral atrophy and different subtypes of intestinal metaplasia (IM); b) the presence of a possible different positive tissue staining for the bacteria in the complete and incomplete intestinal metaplasia. Of the 291 patients, 222 cases (76.3{\%}) showed type I IM, 28 cases (9.6{\%}) type II IM and 41 cases (14.1{\%}) type III IM. Helicobacter-like organisms were found in 42.9{\%} of cases and positive tissue staining rate appeared to be inversely related to the extension of IM (58.7{\%} in IM extended in less than 30{\%} of specimens, 30.2{\%} in IM extended between 30{\%} and 60{\%}, 2.7{\%} in IM exceeding 60{\%} of the biopsed area). The inverse correlation between lower positive tissue staining for Helicobacter-like organisms and greater extension of IM was statistically significant (p <0.001). Incomplete metaplasia appeared to be unrelated to age and associated with a lower positive tissue staining for Helicobacter-like organisms; among patients with type I metaplasia, 118/222 showed Hp-positive bioptic specimens, vs 7/69 of types II and III (p <0.001). In conclusion, positive tissue staining for Helicobacter-like organisms appeared to be significantly (p <0.001) higher in type I IM, vs types II and III, and to a lesser extent to metaplastic changes; in addition, when metaplastic changes are not greatly extended (less than 30{\%}), the presence of the bacteria is significantly reduced in specimens with incomplete form of intestinal metaplasia in comparison with the complete one.",
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AU - Bagnolo, F.

AU - Lella, F.

AU - Buizza, M.

AU - Luinetti, O.

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