Tissue transglutaminase activity protects from cutaneous melanoma metastatic dissemination: An in vivo study

Francesco Facchiano, Daniela D'Arcangelo, Alessandro Lentini, Stefania Rossi, Cinzia Senatore, Tania Pannellini, Claudio Tabolacci, Angelo M. Facchiano, Antonio Facchiano, Simone Beninati

Research output: Contribution to journalArticlepeer-review


The role of tissue transglutaminase (TG-2, TGase-2) in cancer development is still a fascinating field of research. The available reports do not elucidate fully its mechanism of action, due to the limitations of in vitro approaches. Therefore, to understand TG-2 role in cancer, we carried out an in vivo study with a more direct approach. TG-2 was in vivo overexpressed in a murine model of melanoma (intravenous injection of B16 melanoma cells in C57BL/6N mice) by means of a plasmid carrying the TG-2 cDNA. The evaluation of the frequency and size of the metastases indicated that the number of melanoma lung foci was more markedly reduced by TG-2 overexpression than the metastatic size. Then, TG-2 overexpressing mice showed a prolonged survival with respect to control mice. Further analyses were carried by means of proteomic analysis of melanoma cell lysates and meta-analysis of published transcriptomic datasets. Proteomic analysis of cell lysates from a human melanoma cell line compared to human keratinocytes showed significant differences in the expression of TG-2 substrates known to be involved in proliferation/differentiation and cancer progression. Taken together, these findings indicate a protective role of TG-2 enzymatic activity in melanoma progression in vivo.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalAmino Acids
Issue number1
Publication statusPublished - Jan 2013


  • Bioinformatics
  • Cell proteome
  • Cutaneous melanoma
  • Microenvironment
  • Transglutaminase type 2

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry


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