'Tissue' transglutaminase release from apoptotic cells into extracellular matrix during human liver fibrogenesis

Mauro Piacentini, Maria Grazia Farrace, Cesare Hassan, Barbara Serafini, Francesco Autuori

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Enhanced apoptosis characterizes several pathologies affecting human liver. This study sought to determine whether apoptosis is involved in the formation of fibrotic lesions occurring in hepatic disease. The expression of Bcl-2 was analysed, and of 'tissue' transglutaminase (tTG), a cross-linking enzyme which recent evidence suggests plays a role in the formation of fibrotic lesions in experimental settings. Regardless of the degree of liver injury, tTG abnormally accumulated in the liver cells adjacent to fibrotic tissue. Many cells showing DNA fragmentation and morphological features of apoptosis were also observed near fibrotic lesions. Bcl-2 was detected predominantly in infiltrating lymphocytes within the liver tissue. Marked staining for both tTG protein and chromatin was also observed in the acellular fibrotic tissue, which suggested an active release of intracellular macromolecules from the dying cells into the extracellular matrix. This study indicates that fibrogenesis in the liver is associated with the release of tTG from dying cells. By cross-linking extracellular matrix proteins, this enzyme might play a role in the formation of fibrotic lesions.

Original languageEnglish
Pages (from-to)92-98
Number of pages7
JournalJournal of Pathology
Volume189
Issue number1
DOIs
Publication statusPublished - 1999

Fingerprint

Extracellular Matrix
Liver
Apoptosis
Extracellular Matrix Proteins
DNA Fragmentation
Enzymes
Chromatin
transglutaminase 2
Lymphocytes
Pathology
Staining and Labeling
Wounds and Injuries
Proteins

Keywords

  • Apoptosis
  • Bcl-2
  • DNA fragmentation
  • Fibrogenesis
  • Liver
  • Programmed cell death

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

'Tissue' transglutaminase release from apoptotic cells into extracellular matrix during human liver fibrogenesis. / Piacentini, Mauro; Farrace, Maria Grazia; Hassan, Cesare; Serafini, Barbara; Autuori, Francesco.

In: Journal of Pathology, Vol. 189, No. 1, 1999, p. 92-98.

Research output: Contribution to journalArticle

Piacentini, Mauro ; Farrace, Maria Grazia ; Hassan, Cesare ; Serafini, Barbara ; Autuori, Francesco. / 'Tissue' transglutaminase release from apoptotic cells into extracellular matrix during human liver fibrogenesis. In: Journal of Pathology. 1999 ; Vol. 189, No. 1. pp. 92-98.
@article{42e4139ef69249528a6ff78289256461,
title = "'Tissue' transglutaminase release from apoptotic cells into extracellular matrix during human liver fibrogenesis",
abstract = "Enhanced apoptosis characterizes several pathologies affecting human liver. This study sought to determine whether apoptosis is involved in the formation of fibrotic lesions occurring in hepatic disease. The expression of Bcl-2 was analysed, and of 'tissue' transglutaminase (tTG), a cross-linking enzyme which recent evidence suggests plays a role in the formation of fibrotic lesions in experimental settings. Regardless of the degree of liver injury, tTG abnormally accumulated in the liver cells adjacent to fibrotic tissue. Many cells showing DNA fragmentation and morphological features of apoptosis were also observed near fibrotic lesions. Bcl-2 was detected predominantly in infiltrating lymphocytes within the liver tissue. Marked staining for both tTG protein and chromatin was also observed in the acellular fibrotic tissue, which suggested an active release of intracellular macromolecules from the dying cells into the extracellular matrix. This study indicates that fibrogenesis in the liver is associated with the release of tTG from dying cells. By cross-linking extracellular matrix proteins, this enzyme might play a role in the formation of fibrotic lesions.",
keywords = "Apoptosis, Bcl-2, DNA fragmentation, Fibrogenesis, Liver, Programmed cell death",
author = "Mauro Piacentini and Farrace, {Maria Grazia} and Cesare Hassan and Barbara Serafini and Francesco Autuori",
year = "1999",
doi = "10.1002/(SICI)1096-9896(199909)189:1<92::AID-PATH386>3.0.CO;2-B",
language = "English",
volume = "189",
pages = "92--98",
journal = "Journal of Pathology",
issn = "0022-3417",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - 'Tissue' transglutaminase release from apoptotic cells into extracellular matrix during human liver fibrogenesis

AU - Piacentini, Mauro

AU - Farrace, Maria Grazia

AU - Hassan, Cesare

AU - Serafini, Barbara

AU - Autuori, Francesco

PY - 1999

Y1 - 1999

N2 - Enhanced apoptosis characterizes several pathologies affecting human liver. This study sought to determine whether apoptosis is involved in the formation of fibrotic lesions occurring in hepatic disease. The expression of Bcl-2 was analysed, and of 'tissue' transglutaminase (tTG), a cross-linking enzyme which recent evidence suggests plays a role in the formation of fibrotic lesions in experimental settings. Regardless of the degree of liver injury, tTG abnormally accumulated in the liver cells adjacent to fibrotic tissue. Many cells showing DNA fragmentation and morphological features of apoptosis were also observed near fibrotic lesions. Bcl-2 was detected predominantly in infiltrating lymphocytes within the liver tissue. Marked staining for both tTG protein and chromatin was also observed in the acellular fibrotic tissue, which suggested an active release of intracellular macromolecules from the dying cells into the extracellular matrix. This study indicates that fibrogenesis in the liver is associated with the release of tTG from dying cells. By cross-linking extracellular matrix proteins, this enzyme might play a role in the formation of fibrotic lesions.

AB - Enhanced apoptosis characterizes several pathologies affecting human liver. This study sought to determine whether apoptosis is involved in the formation of fibrotic lesions occurring in hepatic disease. The expression of Bcl-2 was analysed, and of 'tissue' transglutaminase (tTG), a cross-linking enzyme which recent evidence suggests plays a role in the formation of fibrotic lesions in experimental settings. Regardless of the degree of liver injury, tTG abnormally accumulated in the liver cells adjacent to fibrotic tissue. Many cells showing DNA fragmentation and morphological features of apoptosis were also observed near fibrotic lesions. Bcl-2 was detected predominantly in infiltrating lymphocytes within the liver tissue. Marked staining for both tTG protein and chromatin was also observed in the acellular fibrotic tissue, which suggested an active release of intracellular macromolecules from the dying cells into the extracellular matrix. This study indicates that fibrogenesis in the liver is associated with the release of tTG from dying cells. By cross-linking extracellular matrix proteins, this enzyme might play a role in the formation of fibrotic lesions.

KW - Apoptosis

KW - Bcl-2

KW - DNA fragmentation

KW - Fibrogenesis

KW - Liver

KW - Programmed cell death

UR - http://www.scopus.com/inward/record.url?scp=0032865222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032865222&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9896(199909)189:1<92::AID-PATH386>3.0.CO;2-B

DO - 10.1002/(SICI)1096-9896(199909)189:1<92::AID-PATH386>3.0.CO;2-B

M3 - Article

C2 - 10451494

AN - SCOPUS:0032865222

VL - 189

SP - 92

EP - 98

JO - Journal of Pathology

JF - Journal of Pathology

SN - 0022-3417

IS - 1

ER -