Tissue-type plasminogen activator as marker of functional steroid receptors in human breast cancer

Carmela Rella, Maria Coviello, Michele Quaranta, Angelo Paradiso

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The relationship between tissue-type plasminogen activator (t-PA) antigen content and receptor status was investigated in 200 human breast cancer cytosols to evaluate the efficacy of t-PA as a marker of functional steroid receptors. t-PA level was measured by an enzyme-linked immunoassay (ELISA) and estrogen (ER) and progesterone (PgR) receptors were assayed by the DCC method. A highly significant correlation was found between t-PA levels and receptor status. The mean ± SE enzyme content was 13.5 ± 2.9 in ER+ tumors and 1.8 ± 0.3 ng/mg protein in ER- tumors; the enzyme content in PgR+ tumors was 14.2 ± 3.3 and 3.4 ± 1.4 ng/mg protein in PgR- tumors. When tumors were divided into four subgroups according to receptor content (ER+PgR+, ER+PgR-, ER-PgR+, and ER-PgR-), t-PA concentration was able to differentiate these groups. Also, t-PA level was compared to several clinical variables; it was not correlated with menopausal status or lymph node involvement. However, t-PA content varied according to tumor size. Our data shows that t-PA content in tumor cytosols is statistically related to receptor status and that determination of t-PA levels in breast cancer might furnish additional information as to the functional state of the receptors which may be necessary for planning hormone therapy.

Original languageEnglish
Pages (from-to)209-220
Number of pages12
JournalThrombosis Research
Volume69
Issue number2
DOIs
Publication statusPublished - Jan 15 1993

Fingerprint

Steroid Receptors
Plasminogen Activators
Tissue Plasminogen Activator
Breast Neoplasms
Progesterone
Estrogens
Neoplasms
Estrogen Receptors
Cytosol
Antigen Receptors
Progesterone Receptors
Enzymes
Immunoenzyme Techniques
Proteins
Lymph Nodes
Hormones

Keywords

  • Breast Cancer
  • Functional Steroid Receptors
  • Tissue-type Plasminogen Activator

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Tissue-type plasminogen activator as marker of functional steroid receptors in human breast cancer. / Rella, Carmela; Coviello, Maria; Quaranta, Michele; Paradiso, Angelo.

In: Thrombosis Research, Vol. 69, No. 2, 15.01.1993, p. 209-220.

Research output: Contribution to journalArticle

Rella, Carmela ; Coviello, Maria ; Quaranta, Michele ; Paradiso, Angelo. / Tissue-type plasminogen activator as marker of functional steroid receptors in human breast cancer. In: Thrombosis Research. 1993 ; Vol. 69, No. 2. pp. 209-220.
@article{c3d2b87043404fa2b8188dc2ea635e46,
title = "Tissue-type plasminogen activator as marker of functional steroid receptors in human breast cancer",
abstract = "The relationship between tissue-type plasminogen activator (t-PA) antigen content and receptor status was investigated in 200 human breast cancer cytosols to evaluate the efficacy of t-PA as a marker of functional steroid receptors. t-PA level was measured by an enzyme-linked immunoassay (ELISA) and estrogen (ER) and progesterone (PgR) receptors were assayed by the DCC method. A highly significant correlation was found between t-PA levels and receptor status. The mean ± SE enzyme content was 13.5 ± 2.9 in ER+ tumors and 1.8 ± 0.3 ng/mg protein in ER- tumors; the enzyme content in PgR+ tumors was 14.2 ± 3.3 and 3.4 ± 1.4 ng/mg protein in PgR- tumors. When tumors were divided into four subgroups according to receptor content (ER+PgR+, ER+PgR-, ER-PgR+, and ER-PgR-), t-PA concentration was able to differentiate these groups. Also, t-PA level was compared to several clinical variables; it was not correlated with menopausal status or lymph node involvement. However, t-PA content varied according to tumor size. Our data shows that t-PA content in tumor cytosols is statistically related to receptor status and that determination of t-PA levels in breast cancer might furnish additional information as to the functional state of the receptors which may be necessary for planning hormone therapy.",
keywords = "Breast Cancer, Functional Steroid Receptors, Tissue-type Plasminogen Activator",
author = "Carmela Rella and Maria Coviello and Michele Quaranta and Angelo Paradiso",
year = "1993",
month = "1",
day = "15",
doi = "10.1016/0049-3848(93)90046-Q",
language = "English",
volume = "69",
pages = "209--220",
journal = "Thrombosis Research",
issn = "0049-3848",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Tissue-type plasminogen activator as marker of functional steroid receptors in human breast cancer

AU - Rella, Carmela

AU - Coviello, Maria

AU - Quaranta, Michele

AU - Paradiso, Angelo

PY - 1993/1/15

Y1 - 1993/1/15

N2 - The relationship between tissue-type plasminogen activator (t-PA) antigen content and receptor status was investigated in 200 human breast cancer cytosols to evaluate the efficacy of t-PA as a marker of functional steroid receptors. t-PA level was measured by an enzyme-linked immunoassay (ELISA) and estrogen (ER) and progesterone (PgR) receptors were assayed by the DCC method. A highly significant correlation was found between t-PA levels and receptor status. The mean ± SE enzyme content was 13.5 ± 2.9 in ER+ tumors and 1.8 ± 0.3 ng/mg protein in ER- tumors; the enzyme content in PgR+ tumors was 14.2 ± 3.3 and 3.4 ± 1.4 ng/mg protein in PgR- tumors. When tumors were divided into four subgroups according to receptor content (ER+PgR+, ER+PgR-, ER-PgR+, and ER-PgR-), t-PA concentration was able to differentiate these groups. Also, t-PA level was compared to several clinical variables; it was not correlated with menopausal status or lymph node involvement. However, t-PA content varied according to tumor size. Our data shows that t-PA content in tumor cytosols is statistically related to receptor status and that determination of t-PA levels in breast cancer might furnish additional information as to the functional state of the receptors which may be necessary for planning hormone therapy.

AB - The relationship between tissue-type plasminogen activator (t-PA) antigen content and receptor status was investigated in 200 human breast cancer cytosols to evaluate the efficacy of t-PA as a marker of functional steroid receptors. t-PA level was measured by an enzyme-linked immunoassay (ELISA) and estrogen (ER) and progesterone (PgR) receptors were assayed by the DCC method. A highly significant correlation was found between t-PA levels and receptor status. The mean ± SE enzyme content was 13.5 ± 2.9 in ER+ tumors and 1.8 ± 0.3 ng/mg protein in ER- tumors; the enzyme content in PgR+ tumors was 14.2 ± 3.3 and 3.4 ± 1.4 ng/mg protein in PgR- tumors. When tumors were divided into four subgroups according to receptor content (ER+PgR+, ER+PgR-, ER-PgR+, and ER-PgR-), t-PA concentration was able to differentiate these groups. Also, t-PA level was compared to several clinical variables; it was not correlated with menopausal status or lymph node involvement. However, t-PA content varied according to tumor size. Our data shows that t-PA content in tumor cytosols is statistically related to receptor status and that determination of t-PA levels in breast cancer might furnish additional information as to the functional state of the receptors which may be necessary for planning hormone therapy.

KW - Breast Cancer

KW - Functional Steroid Receptors

KW - Tissue-type Plasminogen Activator

UR - http://www.scopus.com/inward/record.url?scp=0027502209&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027502209&partnerID=8YFLogxK

U2 - 10.1016/0049-3848(93)90046-Q

DO - 10.1016/0049-3848(93)90046-Q

M3 - Article

C2 - 8383364

AN - SCOPUS:0027502209

VL - 69

SP - 209

EP - 220

JO - Thrombosis Research

JF - Thrombosis Research

SN - 0049-3848

IS - 2

ER -