Titanium and Zirconium levels are associated with changes in MicroRNAs expression: Results from a human cross-sectional study on obese population

Gianguido Cossellu, Valeria Motta, Laura Dioni, Laura Angelici, Luisella Vigna, Gianpietro Farronato, Angela Cecilia Pesatori, Valentina Bollati

Research output: Contribution to journalArticle

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Abstract

Objectives: In this study on 90 individuals we aimed at evaluating the microRNAs (miRNAs) expression profile associated with personal levels of Titanium (Ti) and Zirconium (Zr) traced in hair samples. Ti and Zr materials are broadly used for dental implants but the biological reactions triggeredby a long term presence of these materials in the oral cavity still need to be assessed. MiRNAs are mechanisms that need to be investigated as they play a fundamental role in the control of gene expression following external stimuli and contribute to a wide range of pathophysiological processes. Methods: Using the TaqMan1 Low-Density Array, we assessed the expression levels of 377 human miRNAs in peripheral blood of 90 subjects. Hair samples were analyzed for Ti and Zr content using Inductively Coupled Plasma-Mass Spectrometry. We performed multivariable regression analysis to investigate the effects of Ti and Zr exposure on miRNA expression levels. We used the Ingenuity Pathway Analysis (IPA) software to explore the functional role of the investigated miRNAs and the related target genes. Results: Seven miRNAs (miR-99b, miR-142-5p, miR-152, miR-193a-5p, miR-323-3p, miR-335, miR-494) resulted specifically associated with Zr levels. The functional target analysis showed that miRNAs are involved in mechanisms such as inflammation, skeletal and connective tissue disorders. Conclusions: Our data suggest that Zr is more bioactive than Ti and show that miRNAs are relevant molecular mechanisms sensitive to Zr exposure.

Original languageEnglish
Article numbere0161916
JournalPLoS One
Volume11
Issue number9
DOIs
Publication statusPublished - Sep 1 2016

Fingerprint

titanium
Titanium
MicroRNAs
microRNA
cross-sectional studies
Cross-Sectional Studies
Population
Hair
hairs
Inductively coupled plasma mass spectrometry
Dental prostheses
Dental Implants
atomic absorption spectrometry
Gene expression
Regression analysis
connective tissues
Connective Tissue
Mouth
mouth
Mass Spectrometry

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Titanium and Zirconium levels are associated with changes in MicroRNAs expression : Results from a human cross-sectional study on obese population. / Cossellu, Gianguido; Motta, Valeria; Dioni, Laura; Angelici, Laura; Vigna, Luisella; Farronato, Gianpietro; Pesatori, Angela Cecilia; Bollati, Valentina.

In: PLoS One, Vol. 11, No. 9, e0161916, 01.09.2016.

Research output: Contribution to journalArticle

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abstract = "Objectives: In this study on 90 individuals we aimed at evaluating the microRNAs (miRNAs) expression profile associated with personal levels of Titanium (Ti) and Zirconium (Zr) traced in hair samples. Ti and Zr materials are broadly used for dental implants but the biological reactions triggeredby a long term presence of these materials in the oral cavity still need to be assessed. MiRNAs are mechanisms that need to be investigated as they play a fundamental role in the control of gene expression following external stimuli and contribute to a wide range of pathophysiological processes. Methods: Using the TaqMan1 Low-Density Array, we assessed the expression levels of 377 human miRNAs in peripheral blood of 90 subjects. Hair samples were analyzed for Ti and Zr content using Inductively Coupled Plasma-Mass Spectrometry. We performed multivariable regression analysis to investigate the effects of Ti and Zr exposure on miRNA expression levels. We used the Ingenuity Pathway Analysis (IPA) software to explore the functional role of the investigated miRNAs and the related target genes. Results: Seven miRNAs (miR-99b, miR-142-5p, miR-152, miR-193a-5p, miR-323-3p, miR-335, miR-494) resulted specifically associated with Zr levels. The functional target analysis showed that miRNAs are involved in mechanisms such as inflammation, skeletal and connective tissue disorders. Conclusions: Our data suggest that Zr is more bioactive than Ti and show that miRNAs are relevant molecular mechanisms sensitive to Zr exposure.",
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AU - Dioni, Laura

AU - Angelici, Laura

AU - Vigna, Luisella

AU - Farronato, Gianpietro

AU - Pesatori, Angela Cecilia

AU - Bollati, Valentina

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