Abstract
Background: In light of recent developments in nanotechnologies, interest is growing to better comprehend the interaction of nanoparticles with body tissues, in particular within the cardiovascular system. Attention has recently focused on the link between environmental pollution and cardiovascular diseases. Nanoparticles 2) nanoparticles, the most diffuse nanomaterial in polluted environments and one generally considered inert for the human body. Methods: We conducted functional studies on isolated adult rat cardiomyocytes exposed acutely in vitro to TiO2 and on healthy rats administered a single dose of 2 mg/Kg TiO2 NPs via the trachea. Transmission electron microscopy was used to verify the actual presence of TiO2 nanoparticles within cardiac tissue, toxicological assays were used to assess lipid peroxidation and DNA tissue damage, and an in silico method was used to model the effect on action potential. Results: Ventricular myocytes exposed in vitro to TiO2 had significantly reduced action potential duration, impairment of sarcomere shortening and decreased stability of resting membrane potential. In vivo, a single intra-tracheal administration of saline solution containing TiO2 nanoparticles increased cardiac conduction velocity and tissue excitability, resulting in an enhanced propensity for inducible arrhythmias. Computational modeling of ventricular action potential indicated that a membrane leakage could account for the nanoparticle-induced effects measured on real cardiomyocytes. Conclusions: Acute exposure to TiO2 nanoparticles acutely alters cardiac excitability and increases the likelihood of arrhythmic events.
| Original language | English |
|---|---|
| Pages (from-to) | 1-16 |
| Number of pages | 16 |
| Journal | Particle and Fibre Toxicology |
| DOIs | |
| Publication status | Accepted/In press - Dec 9 2014 |
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Keywords
- Cardiac arrhythmia
- Experimental model
- Membrane leakage
- Pollution
- Supernormal conduction
- Titanium dioxide nanoparticles
ASJC Scopus subject areas
- Health, Toxicology and Mutagenesis
- Toxicology
Cite this
Titanium dioxide nanoparticles promote arrhythmias via a direct interaction with rat cardiac tissue. / Savi, Monia; Rossi, Stefano; Bocchi, Leonardo; Gennaccaro, Laura; Cacciani, Francesca; Perotti, Alessio; Amidani, Davide; Alinovi, Rossella; Goldoni, Matteo; Aliatis, Irene; Lottici, Pier Paolo; Bersani, Danilo; Campanini, Marco; Pinelli, Silvana; Petyx, Marta; Frati, Caterina; Gervasi, Andrea; Urbanek, Konrad; Quaini, Federico; Buschini, Annamaria; Stilli, Donatella; Rivetti, Claudio; Macchi, Emilio; Mutti, Antonio; Miragoli, Michele; Zaniboni, Massimiliano.
In: Particle and Fibre Toxicology, 09.12.2014, p. 1-16.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Titanium dioxide nanoparticles promote arrhythmias via a direct interaction with rat cardiac tissue
AU - Savi, Monia
AU - Rossi, Stefano
AU - Bocchi, Leonardo
AU - Gennaccaro, Laura
AU - Cacciani, Francesca
AU - Perotti, Alessio
AU - Amidani, Davide
AU - Alinovi, Rossella
AU - Goldoni, Matteo
AU - Aliatis, Irene
AU - Lottici, Pier Paolo
AU - Bersani, Danilo
AU - Campanini, Marco
AU - Pinelli, Silvana
AU - Petyx, Marta
AU - Frati, Caterina
AU - Gervasi, Andrea
AU - Urbanek, Konrad
AU - Quaini, Federico
AU - Buschini, Annamaria
AU - Stilli, Donatella
AU - Rivetti, Claudio
AU - Macchi, Emilio
AU - Mutti, Antonio
AU - Miragoli, Michele
AU - Zaniboni, Massimiliano
PY - 2014/12/9
Y1 - 2014/12/9
N2 - Background: In light of recent developments in nanotechnologies, interest is growing to better comprehend the interaction of nanoparticles with body tissues, in particular within the cardiovascular system. Attention has recently focused on the link between environmental pollution and cardiovascular diseases. Nanoparticles 2) nanoparticles, the most diffuse nanomaterial in polluted environments and one generally considered inert for the human body. Methods: We conducted functional studies on isolated adult rat cardiomyocytes exposed acutely in vitro to TiO2 and on healthy rats administered a single dose of 2 mg/Kg TiO2 NPs via the trachea. Transmission electron microscopy was used to verify the actual presence of TiO2 nanoparticles within cardiac tissue, toxicological assays were used to assess lipid peroxidation and DNA tissue damage, and an in silico method was used to model the effect on action potential. Results: Ventricular myocytes exposed in vitro to TiO2 had significantly reduced action potential duration, impairment of sarcomere shortening and decreased stability of resting membrane potential. In vivo, a single intra-tracheal administration of saline solution containing TiO2 nanoparticles increased cardiac conduction velocity and tissue excitability, resulting in an enhanced propensity for inducible arrhythmias. Computational modeling of ventricular action potential indicated that a membrane leakage could account for the nanoparticle-induced effects measured on real cardiomyocytes. Conclusions: Acute exposure to TiO2 nanoparticles acutely alters cardiac excitability and increases the likelihood of arrhythmic events.
AB - Background: In light of recent developments in nanotechnologies, interest is growing to better comprehend the interaction of nanoparticles with body tissues, in particular within the cardiovascular system. Attention has recently focused on the link between environmental pollution and cardiovascular diseases. Nanoparticles 2) nanoparticles, the most diffuse nanomaterial in polluted environments and one generally considered inert for the human body. Methods: We conducted functional studies on isolated adult rat cardiomyocytes exposed acutely in vitro to TiO2 and on healthy rats administered a single dose of 2 mg/Kg TiO2 NPs via the trachea. Transmission electron microscopy was used to verify the actual presence of TiO2 nanoparticles within cardiac tissue, toxicological assays were used to assess lipid peroxidation and DNA tissue damage, and an in silico method was used to model the effect on action potential. Results: Ventricular myocytes exposed in vitro to TiO2 had significantly reduced action potential duration, impairment of sarcomere shortening and decreased stability of resting membrane potential. In vivo, a single intra-tracheal administration of saline solution containing TiO2 nanoparticles increased cardiac conduction velocity and tissue excitability, resulting in an enhanced propensity for inducible arrhythmias. Computational modeling of ventricular action potential indicated that a membrane leakage could account for the nanoparticle-induced effects measured on real cardiomyocytes. Conclusions: Acute exposure to TiO2 nanoparticles acutely alters cardiac excitability and increases the likelihood of arrhythmic events.
KW - Cardiac arrhythmia
KW - Experimental model
KW - Membrane leakage
KW - Pollution
KW - Supernormal conduction
KW - Titanium dioxide nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84928730216&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84928730216&partnerID=8YFLogxK
U2 - 10.1186/s12989-014-0063-3
DO - 10.1186/s12989-014-0063-3
M3 - Article
C2 - 25487314
AN - SCOPUS:84928730216
SP - 1
EP - 16
JO - Particle and Fibre Toxicology
JF - Particle and Fibre Toxicology
SN - 1743-8977
ER -