TNF-α and IFN-γ are overexpressed in the bone marrow of Fanconi anemia patients and TNF-α suppresses erythropoiesis in vitro

Carlo Dufour, Anna Corcione, Johanna Svahn, Riccardo Haupt, Vincenzo Poggi, Albert Nandor Béka'ssy, Rosanna Scimè, Angela Pistorio, Vito Pistoia

Research output: Contribution to journalArticle

Abstract

In Fanconi anemia (FA) C mice tumor necrosis factor α (TNF-(α) and interferon γ (IFN-γ) have key roles in the pathogenesis of bone marrow failure. In FA subjects TNF-α was found to be increased in the serum and overproduced by patient-derived B-cell lines. In acquired aplastic anemia, a disease in which, similarly to FA, marrow failure occurs, TNF-α and IFN-γ act as late mediators of the stem cell damage and are overexpressed in patient marrow lymphocytes. This study evaluated in marrow mononuclear cells (MNCs) of patients with FA, the expression of negative modulators of the hematopoiesis, such as TNF-α, IFN-γ, macrophage inflammatory protein 1α (MIP-1α), and surface Fas ligand, and the role of TNF-α on FA erythropoiesis in vitro. TNF-α and IFN-γ were significantly overexpressed in stimulated marrow MNCs of FA patients as compared to healthy controls. MIP-1α and Fas ligand were undetectable in patients and controls. In bone marrow cultures, the addition of anti-TNF-α. increased the size and significantly increased the number of erythroid colony-forming units and erythroid burst-forming units grown from FA patients but not from healthy controls. This indicates that FA subjects have a marrow TNF-α activity that inhibits erythropoiesis in vitro. TNF-α has a relevant role in the pathogenesis of erythroid failure in FA patients.

Original languageEnglish
Pages (from-to)2053-2059
Number of pages7
JournalBlood
Volume102
Issue number6
DOIs
Publication statusPublished - Sep 15 2003

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ASJC Scopus subject areas

  • Hematology

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