TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured rat hippocampal neurons

Francesca Grassi, Anna Maria Mileo, Lucia Monaco, Antonello Punturieri, Angela Santoni, Fabrizio Eusebi

Research output: Contribution to journalArticle

Abstract

Tumor necrosis factor-α (TNF-α) is a cytokine secreted by activated astrocytes and is known to alter evoked synaptic activity in slices of adult rat hippocampus. In this paper we show that TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured hippocampal neurons, acting at nanomolar concentrations. In addition, we show that the mRNA for the 55 kDa TNF-α receptor (TNF-R1) is detected in embryonic rat hippocampal cultures, as well as in acutely dissected embryonic and adult rat hippocampi. Possible transduction pathways mediating the TNF-α effect are discussed.

Original languageEnglish
Pages (from-to)226-230
Number of pages5
JournalBrain Research
Volume659
Issue number1-2
DOIs
Publication statusPublished - Oct 3 1994

    Fingerprint

Keywords

  • Cultured hippocampal neuron
  • Miniature synaptic current
  • Spontaneous synaptic activity
  • TNF receptor
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this