TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured rat hippocampal neurons

Francesca Grassi, Anna Maria Mileo, Lucia Monaco, Antonello Punturieri, Angela Santoni, Fabrizio Eusebi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Tumor necrosis factor-α (TNF-α) is a cytokine secreted by activated astrocytes and is known to alter evoked synaptic activity in slices of adult rat hippocampus. In this paper we show that TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured hippocampal neurons, acting at nanomolar concentrations. In addition, we show that the mRNA for the 55 kDa TNF-α receptor (TNF-R1) is detected in embryonic rat hippocampal cultures, as well as in acutely dissected embryonic and adult rat hippocampi. Possible transduction pathways mediating the TNF-α effect are discussed.

Original languageEnglish
Pages (from-to)226-230
Number of pages5
JournalBrain Research
Volume659
Issue number1-2
DOIs
Publication statusPublished - Oct 3 1994

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Tumor Necrosis Factor-alpha
Neurons
Hippocampus
Astrocytes
Cytokines
Messenger RNA

Keywords

  • Cultured hippocampal neuron
  • Miniature synaptic current
  • Spontaneous synaptic activity
  • TNF receptor
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured rat hippocampal neurons. / Grassi, Francesca; Mileo, Anna Maria; Monaco, Lucia; Punturieri, Antonello; Santoni, Angela; Eusebi, Fabrizio.

In: Brain Research, Vol. 659, No. 1-2, 03.10.1994, p. 226-230.

Research output: Contribution to journalArticle

Grassi, Francesca ; Mileo, Anna Maria ; Monaco, Lucia ; Punturieri, Antonello ; Santoni, Angela ; Eusebi, Fabrizio. / TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured rat hippocampal neurons. In: Brain Research. 1994 ; Vol. 659, No. 1-2. pp. 226-230.
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