TNF impairs in vivo and in vitro natural killer (NK) susceptibility of B16 melanoma cells

G. Palmieri, S. Morrone, P. L. Lollini, C. De Giovanni, G. Nicoletti, P. Nanni, L. Frati, A. Santoni

Research output: Contribution to journalArticlepeer-review


Tumour necrosis factor α (TNF) is a multipotent cytokine which affects many biological properties of both normal and neoplastic cells. Here we show that treatment with TNF reduces B16-A melanoma cell susceptibility to normal and in vivo- and in vitro-activated NK cell-mediated killing. This resistance is associated with an enhancement of B16-A metastatic potential in normal syngeneic mice, but not in anti-asialo GM 1-treated animals, further supporting the NK dependence of TNF-induced enhancement of metastatic ability. A significant increase of MHC class I expression on B16-A murine melanoma cells is observed after TNF treatment. In all these effects TNF interacts positively with interferon γ (IFN γ). Taken together, these results indicate that TNF treatment negatively affects the susceptibility of B16-A murine melanoma to NK effectors in vivo and in vitro. This decreased susceptibility may be related, at least in part, to enhanced expression of MHC class I antigens on tumour cells.

Original languageEnglish
Pages (from-to)279-287
Number of pages9
JournalScandinavian Journal of Immunology
Issue number3
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Immunology

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