To what extent are the passive electrical parameters of lymphocyte membranes deduced from impedance spectroscopy altered by surface roughness and microvillosity?

C. Cametti, F. de Luca, M. A. Macri', B. Maraviglia, G. Zimatore, F. Bordi, R. Misasi, M. Sorice, L. Lenti, A. Pavan

Research output: Contribution to journalArticlepeer-review

Abstract

The electrical conductivity of human lymphocyte suspensions in NaCl electrolyte solutions of varying osmolarities has been measured in the frequency range from 1 MHz to 1 GHz, where the ion polarization at the membrane-extracellular medium interface influences markedly the electrical behaviour of the cell suspension. To take into account the complexity of the cytoplasmic membrane, where protrusions and microvillous structures extend towards the extracellular medium, an approach based on scaling concepts typical of the fractal analysis of complex systems is proposed. The passive electrical parameters, i.e. the membrane conductivity σs and the membrane permittivity ε{lunate}s, obtained on the basis of this procedure, differ significantly from those derived from conventional methods. The possible relevance of this circumstance from a biological point of view is indicated.

Original languageEnglish
Pages (from-to)309-316
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume3
Issue number5
DOIs
Publication statusPublished - Jan 2 1995

Keywords

  • Impedance spectroscopy
  • Lymphocyte membranes
  • Microvillosity
  • Passive electrical parameters
  • Surface roughness

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

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