Tolerogenic Dendritic Cells That Inhibit Autoimmune Arthritis Can Be Induced by a Combination of Carvacrol and Thermal Stress

Rachel Spiering, Ruurd van der Zee, Josée Wagenaar, Dimos Kapetis, Francesca Zolezzi, Willem van Eden, Femke Broere

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Tolerogenic dendritic cells (DCs) can induce regulatory T cells and dampen pathogenic T cell responses. Therefore, they are possible therapeutic targets in autoimmune diseases. In this study we investigated whether mouse tolerogenic DCs are induced by the phytonutrient carvacrol, a molecule with known anti-inflammatory properties, in combination with a physiological stress. We show that treatment of DCs with carvacrol and thermal stress led to the mRNA expression of both pro- and anti-inflammatory mediators. Interestingly, treated DCs with this mixed gene expression profile had a reduced ability to activate pro-inflammatory T cells. Furthermore, these DCs increased the proportion of FoxP3+ regulatory T cells. In vivo, prophylactic injection of carvacrol-thermal stress treated DCs pulsed with the disease inducing antigen was able to suppress disease in a mouse model of arthritis. These findings suggest that treatment of mouse bone marrow derived DCs with carvacrol and thermal stress induce a functionally tolerogenic DC that can suppress autoimmune arthritis. Herewith carvacrol seems to offer novel opportunities for the development of a dietary based intervention in chronic inflammatory diseases.

Original languageEnglish
Article numbere46336
JournalPLoS One
Volume7
Issue number9
DOIs
Publication statusPublished - Sep 25 2012

Fingerprint

carvacrol
thermal stress
arthritis
dendritic cells
Thermal stress
Dendritic Cells
Arthritis
Hot Temperature
T-cells
T-lymphocytes
Regulatory T-Lymphocytes
Anti-Inflammatory Agents
T-Lymphocytes
Physiological Stress
disease models
autoimmune diseases
mice
Phytochemicals
anti-inflammatory activity
Transcriptome

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Spiering, R., van der Zee, R., Wagenaar, J., Kapetis, D., Zolezzi, F., van Eden, W., & Broere, F. (2012). Tolerogenic Dendritic Cells That Inhibit Autoimmune Arthritis Can Be Induced by a Combination of Carvacrol and Thermal Stress. PLoS One, 7(9), [e46336]. https://doi.org/10.1371/journal.pone.0046336

Tolerogenic Dendritic Cells That Inhibit Autoimmune Arthritis Can Be Induced by a Combination of Carvacrol and Thermal Stress. / Spiering, Rachel; van der Zee, Ruurd; Wagenaar, Josée; Kapetis, Dimos; Zolezzi, Francesca; van Eden, Willem; Broere, Femke.

In: PLoS One, Vol. 7, No. 9, e46336, 25.09.2012.

Research output: Contribution to journalArticle

Spiering, R, van der Zee, R, Wagenaar, J, Kapetis, D, Zolezzi, F, van Eden, W & Broere, F 2012, 'Tolerogenic Dendritic Cells That Inhibit Autoimmune Arthritis Can Be Induced by a Combination of Carvacrol and Thermal Stress', PLoS One, vol. 7, no. 9, e46336. https://doi.org/10.1371/journal.pone.0046336
Spiering, Rachel ; van der Zee, Ruurd ; Wagenaar, Josée ; Kapetis, Dimos ; Zolezzi, Francesca ; van Eden, Willem ; Broere, Femke. / Tolerogenic Dendritic Cells That Inhibit Autoimmune Arthritis Can Be Induced by a Combination of Carvacrol and Thermal Stress. In: PLoS One. 2012 ; Vol. 7, No. 9.
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