Toll-like receptors 3, 4, and 7 are expressed in the enteric nervous system and dorsal root ganglia

Isabella Barajon, Graziano Serrao, Francesca Arnaboldi, Emanuela Opizzi, Gerlomina Ripamonti, Andrea Balsari, Cristiano Rumio

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

The aim of the present study was to evaluate the expression of innate immunity receptors belonging to the Toll-like family in the neural plexuses of the different tracts of murine intestine, of the human ileum, and in lower dorsal root ganglia (DRGs) from where extrinsic afferents to these plexuses originate. Results obtained by immunohistochemistry and immunofluorescence on paraffin-embedded tissue and whole-mount preparations show that Toll-like receptors (TLRs) -3 and -7, recognizing viral RNA, and TLR4, recognizing lipopolysaccharide (membrane component of Gram-negative bacteria), are expressed in the myenteric and submucous plexuses of murine intestine and human ileum, and in DRGs primary sensory neurons. They also show that TLR4 immunostaining is stronger in murine distal large bowel. In murine tissue, expression of TLRs was present in both neurons and glial cells. These observations indicate that the enteric neural network might be directly activated by bacterial and viral components and is therefore more in the forefront than previously envisaged in defense responses of the intestinal wall and in the cross-talk with intestinal microbiota. They also highlight the presence of a peripheral neural network that by way of hardwired neurotransmission could potentially convey to the central nervous system specific information on our microbial counterpart and invading or potentially invading pathogens.

Original languageEnglish
Pages (from-to)1013-1023
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume57
Issue number11
DOIs
Publication statusPublished - Nov 2009

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Toll-Like Receptor 7
Toll-Like Receptor 3
Enteric Nervous System
Toll-Like Receptor 4
Spinal Ganglia
Ileum
Intestines
Submucous Plexus
Myenteric Plexus
Viral Structures
Toll-Like Receptors
Viral RNA
Sensory Receptor Cells
Gram-Negative Bacteria
Innate Immunity
Synaptic Transmission
Neuroglia
Paraffin
Fluorescent Antibody Technique
Lipopolysaccharides

Keywords

  • DRGs
  • ENS
  • Man
  • Mouse
  • TLR3
  • TLR4
  • TLR7
  • Toll-like receptors

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Medicine(all)

Cite this

Toll-like receptors 3, 4, and 7 are expressed in the enteric nervous system and dorsal root ganglia. / Barajon, Isabella; Serrao, Graziano; Arnaboldi, Francesca; Opizzi, Emanuela; Ripamonti, Gerlomina; Balsari, Andrea; Rumio, Cristiano.

In: Journal of Histochemistry and Cytochemistry, Vol. 57, No. 11, 11.2009, p. 1013-1023.

Research output: Contribution to journalArticle

Barajon, Isabella ; Serrao, Graziano ; Arnaboldi, Francesca ; Opizzi, Emanuela ; Ripamonti, Gerlomina ; Balsari, Andrea ; Rumio, Cristiano. / Toll-like receptors 3, 4, and 7 are expressed in the enteric nervous system and dorsal root ganglia. In: Journal of Histochemistry and Cytochemistry. 2009 ; Vol. 57, No. 11. pp. 1013-1023.
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