Tomography system to acquire 3D images of cells in laminar flow: Hardware architecture

G. F. Abate, F. Bavaro, G. Castello, P. Daponte, D. Grimaldi, G. Guglielmelli, U. Martinelli, F. Mauro, S. Moisa, M. Napolitano, S. Rapuano, P. Scerbo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The measurement of the frequency of micronuclei induced in cells by genotoxic agent exposure is widely used for analysis of cytogenetic damage. Nevertheless, the conventional microscope analysis does not provide enough sensitivity, and the manual microscope scoring of micronuclei is a tedious and time-consuming procedure. A new flow-cytometry measurement device with specific hardware and software innovations helps in solving such problem. The paper describes the results of a research project aimed to produce a tomography system able to acquire 3D images of cells in laminar flow. This research was supported by the Italian Ministry of Education, University and Research (MIUR) under the project FIRB 2001 RBAUOIZMZ5. On the basis of this research an innovative Image Flow Cytometer has been designed and prototyped. The innovations concern with both the hardware and the software architecture. The paper focuses on the hardware innovations introduced in the standard cyto-fluorimeter architecture to automatically identify and count the cells including micronuclei. The software innovations are described in a successive paper.

Original languageEnglish
Title of host publicationIEEE International Workshop on Medical Measurement and Applications, MeMeA 2006
Pages68-73
Number of pages6
Volume2006
DOIs
Publication statusPublished - 2006
EventIEEE International Workshop on Medical Measurement and Applications, MeMeA 2006 - Benevento, Italy
Duration: Apr 20 2006Apr 21 2006

Other

OtherIEEE International Workshop on Medical Measurement and Applications, MeMeA 2006
CountryItaly
CityBenevento
Period4/20/064/21/06

Keywords

  • Flow cytometer
  • Laminar flow
  • Micronucleus

ASJC Scopus subject areas

  • Engineering(all)

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