Abstract
The measurement of the frequency of micronuclei induced in cells by genotoxic agent exposure is widely used for analysis of cytogenetic damage. Nevertheless, the conventional microscope analysis does not provide enough sensitivity, and the manual microscope scoring of micronuclei is a tedious and time-consuming procedure. A new flow-cytometry measurement device with specific hardware and software innovations helps in solving such problem. The paper describes the results of a research project aimed to produce a tomography system able to acquire 3D images of cells in laminar flow. This research was supported by the Italian Ministry of Education, University and Research (MIUR) under the project FIRB 2001 RBAUOIZMZ5. On the basis of this research an innovative Image Flow Cytometer has been designed and prototyped. The innovations concern with both the hardware and the software architecture. The paper focuses on the hardware innovations introduced in the standard cyto-fluorimeter architecture to automatically identify and count the cells including micronuclei. The software innovations are described in a successive paper.
| Original language | English |
|---|---|
| Title of host publication | IEEE International Workshop on Medical Measurement and Applications, MeMeA 2006 |
| Pages | 68-73 |
| Number of pages | 6 |
| Volume | 2006 |
| DOIs | |
| Publication status | Published - 2006 |
| Event | IEEE International Workshop on Medical Measurement and Applications, MeMeA 2006 - Benevento, Italy Duration: Apr 20 2006 → Apr 21 2006 |
Other
| Other | IEEE International Workshop on Medical Measurement and Applications, MeMeA 2006 |
|---|---|
| Country | Italy |
| City | Benevento |
| Period | 4/20/06 → 4/21/06 |
Fingerprint
Keywords
- Flow cytometer
- Laminar flow
- Micronucleus
ASJC Scopus subject areas
- Engineering(all)
Cite this
Tomography system to acquire 3D images of cells in laminar flow : Hardware architecture. / Abate, G. F.; Bavaro, F.; Castello, G.; Daponte, P.; Grimaldi, D.; Guglielmelli, G.; Martinelli, U.; Mauro, F.; Moisa, S.; Napolitano, M.; Rapuano, S.; Scerbo, P.
IEEE International Workshop on Medical Measurement and Applications, MeMeA 2006. Vol. 2006 2006. p. 68-73 1644463.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Tomography system to acquire 3D images of cells in laminar flow
T2 - Hardware architecture
AU - Abate, G. F.
AU - Bavaro, F.
AU - Castello, G.
AU - Daponte, P.
AU - Grimaldi, D.
AU - Guglielmelli, G.
AU - Martinelli, U.
AU - Mauro, F.
AU - Moisa, S.
AU - Napolitano, M.
AU - Rapuano, S.
AU - Scerbo, P.
PY - 2006
Y1 - 2006
N2 - The measurement of the frequency of micronuclei induced in cells by genotoxic agent exposure is widely used for analysis of cytogenetic damage. Nevertheless, the conventional microscope analysis does not provide enough sensitivity, and the manual microscope scoring of micronuclei is a tedious and time-consuming procedure. A new flow-cytometry measurement device with specific hardware and software innovations helps in solving such problem. The paper describes the results of a research project aimed to produce a tomography system able to acquire 3D images of cells in laminar flow. This research was supported by the Italian Ministry of Education, University and Research (MIUR) under the project FIRB 2001 RBAUOIZMZ5. On the basis of this research an innovative Image Flow Cytometer has been designed and prototyped. The innovations concern with both the hardware and the software architecture. The paper focuses on the hardware innovations introduced in the standard cyto-fluorimeter architecture to automatically identify and count the cells including micronuclei. The software innovations are described in a successive paper.
AB - The measurement of the frequency of micronuclei induced in cells by genotoxic agent exposure is widely used for analysis of cytogenetic damage. Nevertheless, the conventional microscope analysis does not provide enough sensitivity, and the manual microscope scoring of micronuclei is a tedious and time-consuming procedure. A new flow-cytometry measurement device with specific hardware and software innovations helps in solving such problem. The paper describes the results of a research project aimed to produce a tomography system able to acquire 3D images of cells in laminar flow. This research was supported by the Italian Ministry of Education, University and Research (MIUR) under the project FIRB 2001 RBAUOIZMZ5. On the basis of this research an innovative Image Flow Cytometer has been designed and prototyped. The innovations concern with both the hardware and the software architecture. The paper focuses on the hardware innovations introduced in the standard cyto-fluorimeter architecture to automatically identify and count the cells including micronuclei. The software innovations are described in a successive paper.
KW - Flow cytometer
KW - Laminar flow
KW - Micronucleus
UR - http://www.scopus.com/inward/record.url?scp=33845585237&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845585237&partnerID=8YFLogxK
U2 - 10.1109/MEMEA.2006.1644463
DO - 10.1109/MEMEA.2006.1644463
M3 - Conference contribution
SN - 1424402530
SN - 9781424402533
VL - 2006
SP - 68
EP - 73
BT - IEEE International Workshop on Medical Measurement and Applications, MeMeA 2006
ER -